Mechanical changes in the knee after meniscectomy

Krause, W; Mh, Pope; Rj, Johnson; Wilder, David G.

doi:10.2106/00004623-197658050-00003

Cited by 617 publications

(334 citation statements)

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“…1,2 Meniscus has an important role in load transmission, shock absorption, knee stability, and lubrication of the knee joint. [3][4][5][6] Menisci injury may lead to long-term degenerative joint changes, such as osteophyte formation, articular cartilage degeneration, joint space narrowing, and symptomatic osteoarthritis. 1,2 The treatments of meniscal lesion in knee joint include partial or total meniscectomy, meniscus repair, and allogeneic meniscus transplantation.…”

Section: Introductionmentioning

confidence: 99%

Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model

Kang

Son

Lee

et al. 2006

J Biomedical Materials Res

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Abstract:The current treatments of meniscal lesion in knee joint are not perfect to prevent adverse effects of meniscus injury. Tissue engineering of meniscus using meniscal cells and polymer scaffolds could be an alternative option to treat meniscus injury. This study reports on the regeneration of whole medial meniscus in a rabbit total meniscectomy model using the tissue engineering technique. Biodegradable scaffolds in a meniscal shape were fabricated from polyglycolic acid (PGA) fiber meshes that were mechanically reinforced by bonding PGA fibers at cross points with 75:25 poly(lactic-co-glycolic acid). The compressive modulus of the bonded PGA scaffold was 28-fold higher than that of nonbonded scaffold. Allogeneic meniscal cells were isolated from rabbit meniscus biopsy and cultured in vitro. The expanded meniscal cells were seeded onto the polymer scaffolds, cultured in vitro for 1 week, and transplanted to rabbit knee joints from which medial menisci were removed. Ten or 36 weeks after transplantation, the implants formed neomenisci with the original scaffold shape maintained approximately. Hematoxylin and eosin staining of the sections of the neomenisci at 6 and 10 weeks revealed the regeneration of fibrocartilage. Safranin-O staining showed that abundant proteoglycan was present in the neomenisci at 10 weeks. Masson's trichrome staining indicated the presence of collagen. Immunohistochemical analysis showed that the presence of type I and II collagen in neomenisci at 10 weeks was similar to that of normal meniscal tissue. Biochemical and biomechanical analyses of the tissue-engineered menisci at 36 weeks were performed to determine the quality of the tissue-engineered menisci. Tissue-engineered meniscus showed differences in collagen content and aggregate modulus in comparison with native meniscus. This study demonstrates, for the first time, the feasibility of regenerating whole meniscal cartilage in a rabbit total meniscectomy model using the tissue engineering method.

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Section: Introductionmentioning

confidence: 99%

Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model

Kang

Son

Lee

et al. 2006

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…The meniscus provides several basic functions: weight distribution [2,7,11,16,17,19,22,26], friction reduction [3,12,28], joint stability [26], and shock absorption [3,13,28]. Without the meniscus, the articular cartilage on the ends of the bones would become susceptible to damage which can lead to osteoarthritis.…”

Section: Introductionmentioning

confidence: 99%

Growth factors and fibrochondrocytes in scaffolds

Pangborn

Athanasiou

2005

Journal Orthopaedic Research

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Four growth factors, transforming growth factor-pl (TGF-PI), platelet-derived growth factor-AB (PDGF-AB), insulin-like growth factor I (IGF-I), and basic fibroblastic growth factor (bFGF), were tested at different concentrations for their effects on extracellular matrix (ECM) production in three-dimensional cultures of meniscal fibrochondrocytes. Cells from New Zealand white rabbits were seeded on poly-glycolic acid (PGA) scaffolds and were stimulated with growth factors for three weeks. 3H-proline and 35S-sulfate labels were used to measure uptake of collagen and glycosaminoglycan (GAG) components, respectively. Biochemical assays were performed to measure the total collagen, GAG, and DNA present in the scaffolds at the end of the study. TGF-PI (10 and 100 ng/ml) stimulated both 3H-proline and 3SS-sulfate uptake, showing a dose-dependent response for both and a temporal response for 35S-sulfate uptake. IGF-I (5 ng/ml) and bFGF (25 and 100 ng/ml) showed increases in 3H-proline uptake by the third week of growth factor addition. PDGF-AB did not show notable increases in uptake. Because TGF-p1 (10 and 100 ng/ml) had visibly denser scaffolds, as evidenced by gross microscopy, at lOOx, and the strongest uptake responses to both "S-sulfate and 3H-proline, it appears to be the most effective growth factor for use in scaffold-based approaches to tissue engineer the knee meniscus.

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“…The wedge-shaped menisci increase the congruity between the convex femur and the relatively flat tibia and thereby protect the articular cartilage from excessive axial loads [1][2][3][4][5][6][7][8][9][10] . Radial meniscal tears are not uncommon in young patients 11 , and they often occur at the junction of the body and the posterior horn of the medial meniscus.…”

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confidence: 99%