Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model

Kang, Sung Soo; Son, Sun Mi; Lee, Jae Sun; Lee, Eung Seok; Lee, Kwon Yong; Park, Sang Guk; Park, Jung Ho; Kim, Byung Soo

doi:10.1002/jbm.a.30904

Cited by 63 publications

(87 citation statements)

References 113 publications

(148 reference statements)

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“…Small animal models have been used to provide an assessment of the biological response to new materials implanted into the knee joint [2,16,31]; however, the implants are not subjected to the high loads which would occur in the human knee. Large animal models, such as sheep [18,25], offer the opportunity to subject the candidate materials to immediate and substantial loads, but the models are costly and time consuming to conduct.…”

Section: Introductionmentioning

confidence: 99%

A Pre-Clinical Test Platform for the Functional Evaluation of Scaffolds for Musculoskeletal Defects: The Meniscus

et al. 2011

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In an attempt to delay the progression of osteoarthritis from an index injury, early intervention via repair of injured musculoskeletal soft tissue has been advocated. Despite the development of a number of scaffolds intended to treat soft tissue defects, information about their functional performance is lacking. The goal of this study was to consolidate a suite of in vitro and in vivo models into a pre-clinical test platform to assess the functional performance of meniscal repair scaffolds. Our objective was to assess the ability of a scaffold (Actifit™; Orteq, UK) to carry load without detrimentally abrading against articular cartilage. Three test modules were used to assess the functional performance of meniscal repair scaffolds. The first module tested the ability of the scaffold to carry load in an in vitro model designed to measure the change in normal contact stress magnitude on the tibial plateau of cadaveric knees after scaffold implantation. The second module assessed the in vitro frictional coefficient of the scaffold against cartilage to assess the likelihood that the scaffold would destructively abrade against articular cartilage in vivo. The third module consisted of an assessment of functional performance in vivo by measuring the structure and composition of articular cartilage across the tibial plateau 12 months after scaffold implantation in an ovine model. In vitro, the scaffold improved contact mechanics relative to a partly meniscectomized knee suggesting that, in vivo, less damage would be seen in the scaffold implanted knees vs. partly meniscectomized knees. However, there was no significant difference in the condition of articular cartilage between the two groups. Moreover, in spite of the high coefficient of friction between the scaffold and articular cartilage, there was no significant damage in the articular cartilage underneath the scaffold. The discrepancy between the in vitro and in vivo models was likely influenced by the abundant tissue generated within the scaffold and the unexpected tissue that regenerated within the site of the partial meniscectomy. We are currently augmenting our suite of tests so that we can pre-clinically evaluate the functional performance at time zero and as a function of time after implantation.

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Section: Introductionmentioning

confidence: 99%

A Pre-Clinical Test Platform for the Functional Evaluation of Scaffolds for Musculoskeletal Defects: The Meniscus

et al. 2011

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“…In previous studies, the shape of constructs changed qualitatively from the initial scaffold or mold shape due to growth and/or remodeling of the scaffold during culture [7,22,35]. Additionally, many of the previous reports on shaped cartilaginous grafts did not quantify the construct shapes or contours.…”

Section: Discussionmentioning

confidence: 99%

“…In the scaffold prefabrication approach, a scaffold is shaped into the desired geometry, subsequently seeded with cells (chondrocytes, stem cells, etc. ), and cultured ex vivo or subcutaneously [7,22]. This approach is commonly used for osteochondral grafts, where most of the scaffold is used for the bony substance [35].…”

Section: Introductionmentioning

confidence: 99%

Shaped, Stratified, Scaffold-free Grafts for Articular Cartilage Defects

Han

Bae

Hsieh-Bonassera

et al. 2008

Clin Orthop Relat Res

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One goal of treatment for large articular cartilage defects is to restore the anatomic contour of the joint with tissue having a structure similar to native cartilage. Shaped and stratified cartilaginous tissue may be fabricated into a suitable graft to achieve such restoration. We asked if scaffold-free cartilaginous constructs, anatomically shaped and targeting spherically-shaped hips, can be created using a molding technique and if biomimetic stratification of the shaped constructs can be achieved with appropriate superficial and middle/deep zone chondrocyte subpopulations. The shaped, scaffold-free constructs were formed from the alginate-released bovine calf chondrocytes with shaping on one (saucer), two (cup), or neither (disk) surfaces. The saucer and cup constructs had shapes distinguishable quantitatively (radius of curvature of 5.5 ± 0.1 mm for saucer and 2.8 ± 0.1 mm for cup) and had no adverse effects on the glycosaminoglycan and collagen contents and their distribution in the constructs as assessed by biochemical assays and histology, respectively. Biomimetic stratification of chondrocyte subpopulations in saucer-and cup-shaped constructs was confirmed and quantified using fluorescence microscopy and image analysis. This shaping method, combined with biomimetic stratification, has the potential to create anatomically contoured large cartilaginous constructs.

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“…To date, several cell sources have been used for meniscus repair, including meniscus fibrochondrocytes, chondrocytes or bone marrow-derived mesenchymal progenitor cells (6). However, the use of these cell sources have prompted issues concerning cell selection, acquisition of seed cells and the quality of repaired tissues (7).…”

Section: Introductionmentioning

confidence: 99%

Chondrogenic differentiation of canine myoblasts induced by cartilage-derived morphogenetic protein-2 and transforming growth factor-β1 in vitro

Wang

Dai

et al. 2011

Mol Med Report

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Abstract. The meniscus has limited ability to repair itself following injury. However, tissue engineering provides new means of meniscus repair. Myoblasts, which possess the potential of multi-directional differentiation, may be ideal seed cells in meniscus tissue engineering. Myoblasts from different animals showed slight differences in morphology and in the potential to differentiate. In the present study, we isolated myoblasts from canines and induced chondrogenesis in order to establish a new experimental model of seed cells. Myoblasts were isolated and harvested from Beagle canines. To induce chondrogenesis, cartilage-derived morphogenetic protein-2 (CDMP-2) at different concentrations (10, 20, 50 and 100 ng/ml), transforming growth factor-β1 (TGF-β1; 10, 20, 30 and 50 ng/ml), and different concentrations of CDMP-2 (10, 20, 50 and 100 ng/ml) together with TGF-β1 (20 ng/ml) were added to the cultured pellets. After 21 days of in vitro culture, chondrogenic differentiation was evaluated by histological and immunohistochemical techniques. The degree of gene expression was measured by quantitative RT-PCR. Based on the histological staining of glycosaminoglycan, using the toluidine blue dye-binding method, we found that CDMP-2 initiated chondrogenic differentiation of myoblasts, as did TGF-β1. Furthermore, CDMP-2 conferred a stronger stimulatory effect than TGF-β1. The combination of CDMP-2 and TGF-β1 synergistically induced chondrogenesis of myoblasts. This synergistic chondrogenic effect of CDMP-2 together with TGF-β1 was further confirmed by quantification of glycosaminoglycan using dimethylmethylene blue dye-binding assay and immunohistochemical analysis of the expression of cartilage-specific proteins collagen I and II. Canine myoblasts can be induced into chondrocytes by CDMP-2 and TGF-β1 in vitro, suggesting that myoblasts are suitable as seed cells for meniscus tissue engineering.

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Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model

Cited by 63 publications

References 113 publications

A Pre-Clinical Test Platform for the Functional Evaluation of Scaffolds for Musculoskeletal Defects: The Meniscus

A Pre-Clinical Test Platform for the Functional Evaluation of Scaffolds for Musculoskeletal Defects: The Meniscus

Shaped, Stratified, Scaffold-free Grafts for Articular Cartilage Defects

Chondrogenic differentiation of canine myoblasts induced by cartilage-derived morphogenetic protein-2 and transforming growth factor-β1 in vitro

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