Mechanical cell disruption for lipid extraction from microalgal biomass

Halim, Ronald; Rupasinghe, Thusitha; Tull, Dedreia; Webley, Paul A.

doi:10.1016/j.biortech.2013.04.067

Cited by 127 publications

(46 citation statements)

References 22 publications

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“…Generally, bead milling, high-pressure homogenization, and microwave treatment are energetically among the most costly techniques whereas ultrasound and pulsed electric field are the cheapest (in investments and operational costs). Halim et al (2013) calculated that the actual energy needed for disruption of single cell Tetraselmis suecica and Chlorococcum sp. using high-pressure homogenizer was similar for both species (5.9 Â 10 À5 and 6.4 Â 10 À5 J per cell) whereas a 20 times lower requirement was obtained with ultrasonication.…”

Section: Cost-effectiveness Of Disruption Technologiesmentioning

confidence: 99%

Cell disruption technologies

Dhondt

Martín-Juárez

Bolado

et al. 2017

Microalgae-Based Biofuels and Bioproducts

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IntroductionAlgal cell walls separate the inside cell content from the environment to protect the cell against desiccation, pathogens, and predators while still allowing exchange of compounds. Toward application of algae biomass as a sustainable resource, disruption of this cell wall (¼cell disruption) is an essential pretreatment step to maximize product recovery in downstream processes of the algae biorefinery. Also for direct use of algae in feed or food, cell rupture is required to increase the bioavailability of algae constituents. Depending on the cell wall structure, the size, and the shape of algae, cell disruption can be challenging. A variety of cell disruption methods is currently available, and new approaches are being elaborated in parallel. Since downstream processing is responsible for a large part of the operational costs in the whole production chain, cell disruption technologies should be low cost and energy efficient and result preferably in high product quality. This chapter provides information on cell wall types and gives an overview of physical-mechanical and (bio-)chemical cell disruption technologies with attention to development stage, energy efficiency, product quality, costs, emerging approaches, and applicability on large scale. Cell wall types in various groups of microalgae and cyanobacteriaThe cell wall composition and architecture of algae and cyanobacteria are highly variable ranging from tiny membranes to multilayered complex structures. Despite the importance of algal cell wall properties in biotechnology, little structural information is available for most species (Scholz et al., 2014). Based on the complexity of surface structures, four cell types could be distinguished (Barsanti and Gualtieri, 2006;Lee, 2008) (Fig. 6.1). A simple cell membrane (Fig. 6.1, Type 1) is present in short-lived stages (e.g., gametes), chrysophytes, raphidophytes, green algae Dunaliella, and haptophytes Isochrysis. It consists of a lipid bilayer with integrated and peripheral proteins. Sometimes a cap of glycolipids and glycoproteins envelops the outer surface of cell membrane. Cell membranes with additional extracellular material are known in cyanobacteria and many groups of algae, including palmelloid phases. It is the most diverse cell wall type that includes various membrane-associated structures (cell wall, Microalgae-Based Biofuels and Bioproducts. http://dx

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Section: Cost-effectiveness Of Disruption Technologiesmentioning

confidence: 99%

Cell disruption technologies

Dhondt

Martín-Juárez

Bolado

et al. 2017

Microalgae-Based Biofuels and Bioproducts

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“…Several cell disruption methods (physical, chemical and enzymatic) by wet route are reported in the literature. These include: microwave, ultrasound, acid treatment, sonication, autoclaving and beadbeating [9,10]. For a scale-up, the cell disruption by dry route presents the advantage of reducing the volume of treated product and energy consumption.…”

Section: Introductionmentioning

confidence: 99%

Production of lipids from microalgae Spirulina sp.: Influence of drying, cell disruption and extraction methods

Pohndorf

Camara

Larrosa

et al. 2016

Biomass and Bioenergy

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a b s t r a c tThe pretreatment operations (drying, cell disruption and oil extraction) of microalgae biomass to the lipids extraction are important steps to the product quality and production cost. In this study, the lipids from Spirulina were obtained by different methods of biomass drying (tray and spouted bed), cell disruption (microwave, autoclaving and milling) and solvent extraction (hot and cold). The average content of lipids extracted by the cold method, using polar solvents, was of 5.8 ± 0.6 g 100 g À1 . The spouted bed drying with cell disruption by milling achieved the best performance with the hot extraction method. Under these conditions, the TBA value was of 0.57 ± 0.09 mg MDA kg À1 . FT-IR spectra and thermal analysis indicated that the hot extraction resulted in a more purified lipid extract than the cold extraction. The Brimberg model showed the best fit to the lipid extraction kinetics and the activation energy in the best conditions was of 6.11 ± 1.41 kJ mol À1 .

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“…If the cells are freeze-dried before bead milling, lipid recovery is more due to enhanced specific area and reduced diffusion gradient [64]. Although this method helps in increasing the efficiency, it is very expensive and has high energy consumption.…”

Section: Freeze-drying Methodsmentioning

confidence: 99%

An Insight on Algal Cell Disruption for Biodiesel Production

Aarthy

Smita

Turkar

et al. 2018

Asian J Pharm Clin Res

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Objective: This review article deals with the effect that various cell disruption techniques have on the efficiency of lipid extraction. We have reviewed existing algal cell disruption techniques that aid the biodiesel production process.Methods: Current rise in demand for energy has led the researcher to focus on the production of sustainable fuels, among which biodiesel has received greater attention. This is due to its larger lipid content, higher growth rate, larger biomass production, and lower land use. Extraction of lipid from algae (micro and macro) for the production of biodiesel involves numerous downstream processing steps, of which cell wall disruption is a crucial step. Bead milling, high-pressure homogenization, ultra-sonication, freeze-drying, acid treatment, and enzymatic lysis are some methods of cell disruption. The cell disruption technique needs to be optimized based on the structure and biochemical composition of algae. Result:The lipid extraction efficiency varies depending on the algal species and the cell disruption technique used. Conclusion:In-depth research and development of new techniques are required to further enhance the cell disruption of the algal cell wall for the enhanced recovery of lipids. In addition, the operating costs and energy consumption should also be optimized for the cost-effective recovery.

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Mechanical cell disruption for lipid extraction from microalgal biomass

Cited by 127 publications

References 22 publications

Cell disruption technologies

Cell disruption technologies

Production of lipids from microalgae Spirulina sp.: Influence of drying, cell disruption and extraction methods

An Insight on Algal Cell Disruption for Biodiesel Production

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