Mechanical behavior of human embryonic stem cell pellet under unconfined compression

Ma, Gang; Petersen, Erik F.; Leong, Kam W.; Liao, Kin

doi:10.1007/s10237-011-0344-9

Cited by 5 publications

(3 citation statements)

References 24 publications

(33 reference statements)

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“…Trickey et al measured the mechanical properties of chondrocyte cells via micropipette aspiration and reported a Poisson's ratio of n ¼ 0.38 5 0.06 (30). Finally, Ma et al studied the deformation of stem cell aggregates and deduced that a reasonable value is n ¼ 0.45 (31). Both Trickey and Ma used a finite-element model to predict deformation of the cells or cell aggregates in response to a force.…”

Section: Cell Elastic Propertiesmentioning

confidence: 99%

Combining AFM and Acoustic Probes to Reveal Changes in the Elastic Stiffness Tensor of Living Cells

Nijenhuis

Zhao

Carisey

et al. 2014

Biophysical Journal

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Knowledge of how the elastic stiffness of a cell affects its communication with its environment is of fundamental importance for the understanding of tissue integrity in health and disease. For stiffness measurements, it has been customary to quote a single parameter quantity, e.g., Young's modulus, rather than the minimum of two terms of the stiffness tensor required by elasticity theory. In this study, we use two independent methods (acoustic microscopy and atomic force microscopy nanoindentation) to characterize the elastic properties of a cell and thus determine two independent elastic constants. This allows us to explore in detail how the mechanical properties of cells change in response to signaling pathways that are known to regulate the cell's cytoskeleton. In particular, we demonstrate that altering the tensioning of actin filaments in NIH3T3 cells has a strong influence on the cell's shear modulus but leaves its bulk modulus unchanged. In contrast, altering the polymerization state of actin filaments influences bulk and shear modulus in a similar manner. In addition, we can use the data to directly determine the Poisson ratio of a cell and show that in all cases studied, it is less than, but very close to, 0.5 in value.

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Section: Cell Elastic Propertiesmentioning

confidence: 99%

Combining AFM and Acoustic Probes to Reveal Changes in the Elastic Stiffness Tensor of Living Cells

Nijenhuis

Zhao

Carisey

et al. 2014

Biophysical Journal

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“…Poisson's ratio Density (kg/m³) Embryonic stem cell 0.01 [21] 0.45 [21] 1060 [22] Cell-seeded membrane 0.6 [23] 0.44 [24] 1400 [24] Silicon membrane 150 [25] 0.49 [25] 1030 [26] considered 0.33 Hz and 1Hz which are in the physiological range of the body [14,27]. Besides, To analyze the effect of the stress phase angle, three phases 0, 𝜋 4 ⁄ and 𝜋 2 ⁄ were considered (Figure 2a) [28].…”

Section: Elastic Modulus (Mpa)mentioning

confidence: 99%

In Silico Analysis of Stem Cells Mechanical Stimulations for Mechnoregulation Toward Cardiomyocytes

Ebad

Vahidi

2022

IJE

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Because of the ability of stem cells to self-renew and differentiate into cardiomyocytes, they are optimal cell sources for cardiac tissue engineering. Since heart cells experience cyclic strain and pulsatile flow in vivo, these mechanical stimuli are essential factors for stem cell differentiation. This study aimed to investigate the effect of a combination of pulsatile flow and cyclic strain on the shear stress created on the embryonic stem cell layer with a elastic property in a perfusion bioreactor by using the fluid-solid interaction (FSI) method. In this study, the frequency and stress phase angle had been assumed as a variable. The results show that the maximum shear stress at frequencies of 0.33, and 1 Hz and with frequency differences in cyclic strain (0.33 Hz) and pulsatile flow (1 Hz) are 0.00562, 0.02, and 0.01 dyn/cm², respectively. Moreover, in the stress phase angles 0, 𝜋 4 ⁄ , and 𝜋 2 ⁄ , the maximum shear stress are equal to 0.00562, 0.009, and 0.014 dyn/cm², respectively. The results of this study can be an effective step in developing cardiac tissue engineering and a better understanding of the effects of mechanical stimuli on stem cell differentiation.

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“…However, there are evidence that the Poisson ratio of a cell could range from 0.37 to 0.45, undermining the validity of the constant assumption. [28][29][30] On the other hand, a few studies attempted to determine two independent elastic moduli using one mechanical measurement. For example, a custom cytocompression device that compresses cells against a glass slide using a compressing probe was used to characterize compressive stiffness and the apparent Poisson ratio of chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

On-chip surface acoustic wave and micropipette aspiration techniques to assess cell elastic properties

Cheng

Chen

et al. 2020

Biomicrofluidics

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The cytoskeletal mechanics and cell mechanical properties play an important role in cellular behaviors. In this study, in order to provide comprehensive insights into the relationship between different cytoskeletal components and cellular elastic moduli, we built a phasemodulated surface acoustic wave microfluidic device to measure cellular compressibility and a microfluidic micropipette-aspiration device to measure cellular Young's modulus. The microfluidic devices were validated based on experimental data and computational simulations. The contributions of structural cytoskeletal actin filament and microtubule to cellular compressibility and Young's modulus were examined in MCF-7 cells. The compressibility of MCF-7 cells was increased after microtubule disruption, whereas actin disruption had no effect. In contrast, Young's modulus of MCF-7 cells was reduced after actin disruption but unaffected by microtubule disruption. The actin filaments and microtubules were stained to confirm the structural alteration in cytoskeleton. Our findings suggest the dissimilarity in the structural roles of actin filaments and microtubules in terms of cellular compressibility and Young's modulus. Based on the differences in location and structure, actin filaments mainly contribute to tensile Young's modulus and microtubules mainly contribute to compressibility. In addition, different responses to cytoskeletal alterations between acoustophoresis and micropipette aspiration demonstrated that micropipette aspiration was better at detecting the change from actin cortex, while the response to acoustophoresis was governed by microtubule networks.

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Mechanical behavior of human embryonic stem cell pellet under unconfined compression

Cited by 5 publications

References 24 publications

Combining AFM and Acoustic Probes to Reveal Changes in the Elastic Stiffness Tensor of Living Cells

Combining AFM and Acoustic Probes to Reveal Changes in the Elastic Stiffness Tensor of Living Cells

In Silico Analysis of Stem Cells Mechanical Stimulations for Mechnoregulation Toward Cardiomyocytes

On-chip surface acoustic wave and micropipette aspiration techniques to assess cell elastic properties

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