Meat Adulteration in Cooked Mutton Kebab with Cattle and Buffalo Meat and its Detection Using Mitochondrial DNA (mtDNA) Based Multiplex PCR

Bhat, M. M.; Mantoo, Imtiyaz A.; Salahuddin, Mir; Adil, S.; Pal, Mohammad Ashraf

doi:10.3923/ajava.2016.505.510

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…The first official control performed by the Agricultural and Food Quality Inspection in Poland took place in 2023 and showed many irregularities [ 17 ]. In the literature, authors focus on the study of an effective method of analyzing the authenticity of kebabs [ 4 , 9 , 18 ]. Only one study disclosed several systematically deficient regulatory requirements in Döner kebab labelling [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Do You Know What You Eat? Kebab Adulteration in Poland

Szyłak,

Kostrzewa,

Bania

et al. 2023

Foods

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In recent years, consumer interest in meat authenticity has increased. Fraudulent claims are most likely to be regarding meat origin, meat substitution, meat processing treatment, and non-meat ingredient additions. This study focuses on the substitution of meat species in processed kebab-like food sales in Poland. The growing popularity of kebab-like foods and the limited number of official inspections of this type of food make this topic interesting. In this study, the results reveal that 60% of the foods analyzed contain an undeclared ingredient or the substitution of an expensive ingredient with a cheaper option.

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Section: Discussionmentioning

confidence: 99%

Do You Know What You Eat? Kebab Adulteration in Poland

Szyłak,

Kostrzewa,

Bania

et al. 2023

Foods

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“…(2019). It can be safely stated that mPCR technologies, despite their limitations, have been successfully used by many authors to identify various game animals, for example, crocodile (Ahmad Nizar et al., 2018), buffalo (Mansoor Bhat et al., 2016), lamb, rabbit, and squirrel (Ahamad et al., 2017), horse and donkey (Alikord et al., 2017), yak (Zhao, Xu, et al., 2019a), and deer (Kim et al., 2020).…”

Section: Dna‐based Methods For Game Species Identificationmentioning

confidence: 99%

A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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Despite the numerous studies on food safety and authenticity, especially for meat and meat products, not enough studies have been conducted focusing exclusively on game species and other unusual meat animals. As a result of the European horse scandal, the horse is currently the target of many meat authenticity studies. With this review, we aim to present various DNA-based methods that have been used by researchers to identify, detect, and quantify game, uncommon meat animals, and wildlife species. Polymerase chain reaction (PCR) is considered the standard method for DNA analysis in meat authenticity testing. However, in this paper, we present several other methods that may or may not involve the PCR technique. For this purpose, we systematically reviewed 131 articles selected according to various criteria such as target animal species, method of analysis, year of publication, and so forth. The result of our study shows the most studied game and uncommon meat species, PCR-and non-PCR-based methods for game meat analysis, and DNA-based methods in wildlife conservation. With this study, researchers can find detailed information about frequent game species used as adulterants for regular meat products and the DNA-based techniques to identify them.

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“…Through the advance of biotechnology and research, numerous identification methods for meat species identification have been developed, and todate, two main methods are recognized for meat species detection and identification in food, which are protein-and DNA-based methods [4,5]. Proteinbased methods, however, have limitations in the detection of meat species from cooked or heat-treated food products since proteins can be denatured during processing [6,7,8]. DNA-based methods such as Polymerase Chain Reaction (PCR) are the most precise and sensitive techniques for species identification, and they are comparatively quick compared to protein-based methods [9,10].…”

Section: Introductionmentioning

confidence: 99%

Efficiency of Traditional Dna Extraction Method in PCR Detection of Porcine Dna in Meat Mixtures

et al. 2020

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Through the advancement of biotechnology, DNA-based methods are the most effective techniques in species identification, as they are rapid and have higher stability in harsh conditions compared to protein-based methods. This study was conducted to determine the efficiency of the traditional DNA extraction method, phenol/chloroform/isoamyl alcohol (PCIA), and comparing it with the commercially available kit by evaluating the purity, concentration, and suitability for amplification of porcine DNA in raw chicken and beef mixtures. The quantity and quality of the DNA extracts were assessed using a UV-Vis spectrophotometer. Polymerase chain reaction (PCR) was performed using species-specific primers targeting mitochondrial DNA cytochrome b (cyt b) gene of chicken (227-bp), beef (274-bp), and pork (398-bp), to confirm the template usability and quality of the DNA extracts. High DNA concentrations and purity were obtained from meat samples extracted using the PCIA method. The visualization of pork DNA on 2% agarose gel was able to detect pork contamination in raw meat mixtures up to minute proportion (1%). The existence of pork in chicken and beef was indicated with the presence of a specific 398-bp DNA band. Thus, the PCIA method can be recommended as a cost-effective and an excellent alternative to more expensive extraction kits in detecting pork DNA in raw meat mixtures.

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Meat Adulteration in Cooked Mutton Kebab with Cattle and Buffalo Meat and its Detection Using Mitochondrial DNA (mtDNA) Based Multiplex PCR

Cited by 4 publications

References 22 publications

Do You Know What You Eat? Kebab Adulteration in Poland

Do You Know What You Eat? Kebab Adulteration in Poland

A systematic review of DNA‐based methods in authentication of game and less common meat species

Efficiency of Traditional Dna Extraction Method in PCR Detection of Porcine Dna in Meat Mixtures

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