Measuring the genetic structure of the pollen pool as the probability of paternal identity

Smouse, Peter E.; Robledo‐Arnuncio, Juan J.

doi:10.1038/sj.hdy.6800674

Cited by 44 publications

(46 citation statements)

References 37 publications

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“…To identify whether seed parents were exposed to the same pollen pool, the degree of pollen source overlap between pairs of seed parents ( r ij ) was estimated following Smouse and Robledo‐Arnuncio (2005). …”

Section: Methodsmentioning

confidence: 99%

Limiting inbreeding in disjunct and isolated populations of a woody shrub

Sampson

Byrne

Gibson

et al. 2016

Ecology and Evolution

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Pollen movements and mating patterns are key features that influence population genetic structure. When gene flow is low, small populations are prone to increased genetic drift and inbreeding, but naturally disjunct species may have features that reduce inbreeding and contribute to their persistence despite genetic isolation. Using microsatellite loci, we investigated outcrossing levels, family mating parameters, pollen dispersal, and spatial genetic structure in three populations of Hakea oldfieldii, a fire‐sensitive shrub with naturally disjunct, isolated populations prone to reduction in size and extinction following fires. We mapped and genotyped a sample of 102 plants from a large population, and all plants from two smaller populations (28 and 20 individuals), and genotyped 158–210 progeny from each population. We found high outcrossing despite the possibility of geitonogamous pollination, small amounts of biparental inbreeding, a limited number of successful pollen parents within populations, and significant correlated paternity. The number of pollen parents for each seed parent was moderate. There was low but significant spatial genetic structure up to 10 m around plants, but the majority of successful pollen came from outside this area including substantial proportions from distant plants within populations. Seed production varied among seven populations investigated but was not correlated with census population size. We suggest there may be a mechanism to prevent self‐pollination in H. oldfieldii and that high outcrossing and pollen dispersal within populations would promote genetic diversity among the relatively small amount of seed stored in the canopy. These features of the mating system would contribute to the persistence of genetically isolated populations prone to fluctuations in size.

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Section: Methodsmentioning

confidence: 99%

Limiting inbreeding in disjunct and isolated populations of a woody shrub

Sampson

Byrne

Gibson

et al. 2016

Ecology and Evolution

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“…Also using the paternity assignments for individual maternal trees estimated above, we calculated the PPI following Smouse and Robledo-Arnuncio (2005). This approach involves calculation of two estimators: r gg , which provides an estimate of the degree of pollen source overlap within a single maternal tree (and which can be NS, non-significant; P ID , probability of identity; P EX , probability of parentage exclusion (one parent known); P ID C, combined probability of identity, P EX C, combined probability of parentage exclusion; Error, genotyping error rate (%); NS, *Po0.05).…”

Section: Discussionmentioning

confidence: 99%

“…By identifying and genotyping all potential pollen sources within a sufficiently large study area, direct paternity assignment can be used to categorically identify the male parent of individual seeds, and thus provides insight into how individual traits of contributing males or ecological context may affect the relationship between dispersal distance and N e . Paternal diversity measures (probability of paternal identity, (PPI) Smouse and Robledo-Arnuncio, 2005) enable us to measure the degree of sharing of pollen donors amongst individual female trees and to estimate effective number of sires. Further, spatially-explicit mating models are available to infer the individual dispersal kernel, which provides important information on the shape of the dispersal tail (Burczyk et al, 2002;Oddou-Muratorio et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The pollen dispersal kernel and mating system of an insect-pollinated tropical palm, Oenocarpus bataua

et al. 2012

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Pollen dispersal shapes the local genetic structure of plant populations and determines the opportunity for local selection and genetic drift, but has been well studied in few animal-pollinated plants in tropical rainforests. Here, we characterise pollen movement for an insect-pollinated Neotropical canopy palm, Oenocarpus bataua, and relate these data to adult mating system and population genetic structure. The study covers a 130-ha parcel in which all adult trees (n ¼ 185) were mapped and genotyped at 12 microsatellite loci, allowing us to positively identify the source tree for 90% of pollination events (n ¼ 287 of 318 events). Mating system analysis showed O. bataua was effectively outcrossed (t m ¼ 1.02) with little biparental inbreeding (t m Àt s ¼ À0.005) and an average of 5.4 effective pollen donors (N ep ) per female. Dispersal distances were relatively large for an insect-pollinated species (mean ¼ 303 m, max ¼ 1263 m), and far exceeded nearest-neighbour distances. Dispersal kernel modelling indicated a thin-tailed Weibull distribution offered the best fit to the genetic data, which contrasts with the fat-tailed kernels typically reported for pollen dispersal in trees. Preliminary analyses suggest that our findings may be explained, at least in part, by a relatively diffuse spatial and temporal distribution of flowering trees. Comparison with previously reported estimates of seed movement for O. bataua suggests that pollen and seed dispersal distances may be similar. These findings add to the growing body of information on dispersal in insect-pollinated trees, but underscore the need for continued research on tropical systems in general, and palms in particular. Keywords: Arecaceae; Chocó rainforest; chapil palm; neighbourhood model; phenology; spatial genetic structure; weibull distribution INTRODUCTION Pollination biology is a key determinant of short-term microevolutionary processes in plants. Pollen movement, along with seed dispersal, drives the distribution of genetic diversity in plant populations and is important for generating within-and between-population genetic structure. Wright (1943) recognised that restricted dispersal could lead to population sub-division as an outcome of restricted propagule movement, and introduced the concept of neighbourhood size (N e ) to describe the effective number of randomly mating individuals in a population area. Neighbourhood size has become a common metric of the scale and effectiveness of dispersal, and determining how pollination biology affects N e is a fundamental goal in plant ecology and genetics (Crawford, 1984).The average and maximum distances pollen travels between the pollen source and maternal tree are often used as an index of neighbourhood size by plant biologists (Levin and Kerster, 1971). The focus on distance per se in pollination biology can be related to the early observation that pollen dispersal kernels (that is, the probability density function of propagule dispersal distances from individual plants) typically follow a leptokurtic dis...

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“…2a in Smouse and Robledo-Arnuncio 2005). The diversity of pollen donors that mated with the ith mother genet (D i ) was represented as:…”

Section: Mating Patternsmentioning

confidence: 99%

Effect of population density of compatible neighbours on inbreeding level within a Primula sieboldii population

Kitamoto

Ueno

Tsumura

et al. 2007

Ecological Research

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We investigated the effect of population density of compatible neighbours on inbreeding level of Primula sieboldii, a heterostylous clonal herb. Pollinator availability, seed set, selfing rate, diversity of pollen donors, and fitness of progenies were compared between less and more isolated genets, which differed in the number of compatible opposite-morph genets within 20 m, the range at which most pollen flow occurred. Although pollinator availability did not differ between the two groups, seed set and diversity of pollen donors in more isolated genets were significantly lower than in less isolated genets. Additionally, the mean selfing rates of less and more isolated genets were 1.3 and 36.7%, respectively, and the mean leaf area of the self-fertilized seedlings was 70 to 40% smaller than that of outcrossed seedlings of the same mother genet. Due to this large inbreeding depression, it is unlikely that self-fertilized seedlings could successfully establish in natural habitats and hence the inbreeding level in the next generation around the more isolated genet would not increase rapidly. However, the possibility of mating between fullsibs would increase because the diversity of pollen donors was low and both pollen and seed dispersal were spatially restricted. Thus the inbreeding level of the next generations would gradually increase around the more isolated genets owing to biparental inbreeding. This study suggested that the population density of compatible neighbours has a critical impact on the future inbreeding level within P. sieboldii populations.

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Measuring the genetic structure of the pollen pool as the probability of paternal identity

Cited by 44 publications

References 37 publications

Limiting inbreeding in disjunct and isolated populations of a woody shrub

Limiting inbreeding in disjunct and isolated populations of a woody shrub

The pollen dispersal kernel and mating system of an insect-pollinated tropical palm, Oenocarpus bataua

Effect of population density of compatible neighbours on inbreeding level within a Primula sieboldii population

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