Measuring the bioactivity and molecular conformation of typically globular proteins with phenothiazine-derived methylene blue in solid and in solution: A comparative study using photochemistry and computational chemistry

Ding, Fei; Xie, Yong; Peng, Wei; Peng, Yu-Kui

doi:10.1016/j.jphotobiol.2016.02.029

Cited by 15 publications

(12 citation statements)

References 84 publications

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“…The interaction of ligands and complexes ( 1 ) and ( 2 ) with DNA has been recorded by the use of MB. MB is a well‐known intercalator and tricyclic that is often used as a spectral probe to establish the mode of binding of small molecules to double‐helical DNA. The emission spectra of the MB‐DNA solution are shown in Figure by the addition of various amounts of ligands and complexes ( 1 ) and ( 2 ).…”

Section: Resultsmentioning

confidence: 99%

Spectroscopic studies, structural characterization and electrochemical studies of two cobalt (III) complexes with tridentate hydrazone Schiff base ligands: Evaluation of antibacterial activities, DNA‐binding, BSA interaction and molecular docking

Fekri

Salehi

Asadi

et al. 2017

Applied Organom Chemis

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Co(III) complexes of tridentate Schiff base ligands derived from N‐(2‐hydroxybenzylideneamino)benzamide (H2L1) and 2‐(2‐hydroxybenzylidene)hydrazine‐1‐carboxamide (H2L2) were synthesized and characterized using IR, Raman, 1H–NMR and UV–Vis spectroscopies. X‐ray single crystal structures of complexes 1 and 2 have also been determined, and it was indicated that these Co(III) complexes are in a distorted octahedral geometry. The cyclic voltammetry (CV) of the complexes indicates an irreversible redox behavior for both complexes 1 and 2. The antibacterial effects of the synthesized compounds have been tested by minimum inhibitory concentration and minimum bactericidal concentration methods, which suggested that the metal complexes exhibit better antibacterial effects than the ligands against Gram‐positive bacteria. The effects of the drug (drug = ligands and complexes) on bovine serum albumin (BSA) were examined using circular dichroism (CD) spectropolarimetry, and it was revealed that the BSA (BSA, as a carrier protein) secondary structure changed in the presence of the drug. Interaction of the drug with calf‐thymus DNA (CT‐DNA) was investigated by UV–Vis absorption, fluorescence emission, CV and CD spectroscopy. Binding constants were determined using UV–Vis absorption. The results indicated that the studied Schiff bases bind to DNA, with the hyperchromic effect and non‐intercalative mode in which the metal complexes are more effective than ligands. Furthermore, molecular docking simulation was used to obtain the energetic and binding sites for the interaction of the complexes with Mycobacterium tuberculosis enoyl‐acyl carrier protein reductase (InhA), and results showed that complex 1 has more binding energy.

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Section: Resultsmentioning

confidence: 99%

Spectroscopic studies, structural characterization and electrochemical studies of two cobalt (III) complexes with tridentate hydrazone Schiff base ligands: Evaluation of antibacterial activities, DNA‐binding, BSA interaction and molecular docking

Fekri

Salehi

Asadi

et al. 2017

Applied Organom Chemis

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“…Of note, it has been shown with other proteins that MB affords a large quenching of Trp fluorescence upon the binding of MB to protein sites close to accessible Trp(s), mainly by static quenching [73,74]. In the presence of tau, the MB-induced quenching of the intrinsic fluorescence of PMCA purified from pig brain or purified hPMCA4b reconstituted in PS or PC is largely enhanced with a significant increase of PMCA affinity for MB.…”

Section: Discussionmentioning

confidence: 99%

Methylene Blue Blocks and Reverses the Inhibitory Effect of Tau on PMCA Function

Berrocal

Caballero-Bermejo

Gutiérrez‐Merino

et al. 2019

IJMS

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Methylene blue (MB) is a synthetic phenothiazine dye that, in the last years, has generated much debate about whether it could be a useful therapeutic drug for tau-related pathologies, such as Alzheimer’s disease (AD). However, the molecular mechanism of action is far from clear. Recently we reported that MB activates the plasma membrane Ca2+-ATPase (PMCA) in membranes from human and pig tissues and from cells cultures, and that it could protect against inactivation of PMCA by amyloid β-peptide (Aβ). The purpose of the present study is to further examine whether the MB could also modulate the inhibitory effect of tau, another key molecular marker of AD, on PMCA activity. By using kinetic assays in membranes from several tissues and cell cultures, we found that this phenothiazine was able to block and even to completely reverse the inhibitory effect of tau on PMCA. The results of this work point out that MB could mediate the toxic effect of tau related to the deregulation of calcium homeostasis by blocking the impairment of PMCA activity by tau. We then could conclude that MB could interfere with the toxic effects of tau by restoring the function of PMCA pump as a fine tuner of calcium homeostasis.

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“…It is also used in various industries, such as textile, paper paints, hair color, etc. Intense exposure to MB leads to severe damages to the human body (2,5). MB belongs to azo dyes family which contain the functional group R-N=N-R′ in their structure and constitute a very large group of all coloring agents used.…”

Section: Introductionmentioning

confidence: 99%

“…Even for nonallergic persons, they may be the cause of, for example, urticaria (6). Therefore, due to environmental problems and human health, treatment of wastewater containing these compounds has become a vital issue (5).…”

Section: Introductionmentioning

confidence: 99%

Optimizing the removal of methylene blue from aqueous solutions using persulfate activated with nanoscale zero valent iron (nZVI) supported by reduced expanded graphene oxide (rEGO)

Soubh

Abdoli

Ahmad

2021

Environ Health Eng Manag

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Background: To remove methylene blue (MB) from aqueous solutions, nanoscale zero-valent iron (nZVI) predicated on reduced expanded graphene oxide (rEGO) was used as the activator of persulfate. Methods: Scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) analyses were used to investigate the surface morphology and to examine the surface elemental composition. X-ray diffraction (XRD) was used to determine the chemical compositions of the synthesized compound. In this study, the effects of pH (3-9), activator dose (0.4-1.6 g L-1), persulfate concentration (0.192-0.768 g L-1), and reaction time (0-60 minutes) on the removal of 10 mg L-1 MB were studied by nZVI -reduced expanded graphene oxide/persulfate (nZVI@rEGO/PS) process. Results: The maximum removal efficiencies of MB at optimum operational conditions (pH 3, activator dose = 1.2 g L-1, persulfate concentration = 0.576 g L-1, and reaction time = 20 minutes) by nZVI@rEGO/PS process was 96%. The chemical method was used to prepare expanded graphene. The volume of natural flake graphite increased about 25 times after the process. SEM image of the nZVI@rEGO showed the presence of nZVI placed on the EGO surface in chain structure with a diameter about 100 nm. The EDS analysis of the activator indicated the existence of Fe element to an amount greater than 50%. Conclusion: According to the results, nZVI@rEGO is considered as a promising activator of persulfate.

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Measuring the bioactivity and molecular conformation of typically globular proteins with phenothiazine-derived methylene blue in solid and in solution: A comparative study using photochemistry and computational chemistry

Cited by 15 publications

References 84 publications

Spectroscopic studies, structural characterization and electrochemical studies of two cobalt (III) complexes with tridentate hydrazone Schiff base ligands: Evaluation of antibacterial activities, DNA‐binding, BSA interaction and molecular docking

Spectroscopic studies, structural characterization and electrochemical studies of two cobalt (III) complexes with tridentate hydrazone Schiff base ligands: Evaluation of antibacterial activities, DNA‐binding, BSA interaction and molecular docking

Methylene Blue Blocks and Reverses the Inhibitory Effect of Tau on PMCA Function

Optimizing the removal of methylene blue from aqueous solutions using persulfate activated with nanoscale zero valent iron (nZVI) supported by reduced expanded graphene oxide (rEGO)

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