Measuring platelet aggregation to estimate small intestinal ischemia-reperfusion injury

Arakawa, Kazuhisa; Takeyoshi, Izumi; Muraoka, Masako; Matsumoto, Koshi; Morishita, Yasuo

doi:10.1016/j.jss.2004.07.011

Cited by 16 publications

(5 citation statements)

References 24 publications

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“…The main mechanisms of thrombocytopenia in septic rats include bone marrow suppression and neonatal thrombocytopenia, immune-mediated platelet destruction, and platelet depletion [35]. However, in the rat model of intestinal I/R injury, there were no statistically significant differences in platelet count among all groups, which was consistent with a previous study [36]. We speculated that the cause might be (at least in part) inflammatory exudation after intestinal I/R, which leads to blood concentration, abnormal distribution of platelets in the body, platelet aggregation, and increased attachment.…”

supporting

confidence: 90%

Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB/NLRP3 Pathway

Yang

Guo

Fan

et al. 2020

Mediators of Inflammation

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Intestinal ischemia/reperfusion (I/R) injury often causes inflammatory responses and coagulation disorders, which is further promoting the deterioration of the disease. Hydrogen has anti-inflammatory, antioxidative, and antiapoptotic properties against various diseases. However, the effect of hydrogen on coagulation dysfunction after intestinal I/R and the underlying mechanism remains unclear. The purpose of this study was to explore whether hydrogen-rich solution (HRS) could attenuate coagulation disorders and inflammation to improve intestinal injury and poor survival following intestinal I/R. The rat model of intestinal I/R injury was established by clamping the superior mesenteric artery for 90 min and reperfusion for 2 h. HRS (10 or 20 mL/kg) or 20 mL/kg 0.9% normal saline was intravenously injected at 10 min before reperfusion, respectively. The samples were harvested at 2 h after reperfusion for further analyses. Moreover, the survival rate was observed for 24 h. The results showed that HRS improved the survival rate and alleviated serum diamine oxidase activities, intestinal injury, edema, and apoptosis. Interestingly, HRS markedly improved intestinal I/R-mediated coagulation disorders as evidenced by abnormal conventional indicators of coagulation and thromboelastography. Additionally, HRS attenuated inflammatory responses and the elevated tissue factor (TF) and inhibited nuclear factor kappa beta (NF-κB) and nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome activation in peripheral blood mononuclear cells. Moreover, inflammatory factors and myeloperoxidase were closely associated with TF level. This study thus emphasized upon the amelioration of coagulation disorders and inflammation by HRS as a mechanism to improve intestinal I/R-induced intestinal injury and poor survival, which might be partially related to inhibition of NF-κB/NLRP3 pathway.

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supporting

confidence: 90%

Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB/NLRP3 Pathway

Yang

Guo

Fan

et al. 2020

Mediators of Inflammation

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“…Thrombotic and inflammatory statuses of AMI have been evaluated using several biomarkers such as C-reactive protein (CRP) and mean platelet volume [4][5][6][7]. Recently, neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR), which can be derived from the complete blood count, have been studied as novel markers of prognosis in patients with ischemic diseases [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Novel hematologic inflammatory parameters to predict acute mesenteric ischemia

Toptaş

Akkoç

Savas

et al. 2016

Blood Coagulation &Amp; Fibrinolysis

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Acute mesenteric ischaemia (AMI) is an emergency condition that requires urgent diagnosis. Neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) have been studied as inflammatory biomarkers in atherosclerosis, but data regarding AMI are lacking. The study population included patients with AMI (n = 46) versus age and sex-matched healthy controls (n = 46). Computed multidetector tomographic angiography was performed to diagnose AMI. NLR and PLR were calculated using complete blood count. C-reactive protein (CRP) levels were also analyzed. Neutrophil levels and lymphocytes were significantly higher in patients with AMI than in the control individuals (P < 0.001 and P = 0.43, respectively). NLR levels were significantly higher in patients with AMI compared with that in the control individuals (P < 0.001). Platelet levels did not reach statistical significance between the groups (P = 0.709). However, patients with AMI had significantly higher PLR levels than the control group (P = 0.039). CRP levels on admission were higher in patients with AMI in comparison with control individuals. There was also a positive correlation between NLR and CRP (r = 0.548, P < 0.001), and between PLR and CRP (r = 0.528, P < 0.001). NLR level greater than 4.5, measured on admission, yielded an area under the curve value of 0.790 (95% confidence interval 0.681-0.799, sensitivity 77%, specificity 72%), and PLR level of greater than 157 yielded an area under the curve value of 0.604 (95% confidence interval 0.486-0.722, sensitivity 59%, specificity 65%). Patients with AMI had increased NLR, PLR, and CRP levels compared with controls. Increased NLR and PLR was an independent predictor of AMI.

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“…The mesenteric blood flow reduction and the ischemiareperfusion injury plays an important role in the pathogenesis and survival of many clinical and surgical diseases [9][10][11] , with important research approach done [12][13][14][15] to understand the involved mechanisms aiming to obtain the best protection. Although confirmed in many researches, the pre and postconditioning failure to avoid the ischemia and ischemia-reperfusion lesions is also reported [16][17][18][19] , raising the respiratory dysfunction after cardiopulmonary bypass 20 and to brain stroke events when induced by proton pump Na This relative contradictory scenario regarding results with ischemic and or drug induced preconditioning was consistently recently studied by Ramzy et al 22 pointing the importance and benefits of new researches for best clinical results with pre and postconditioning.…”

Section: Discussionmentioning

confidence: 99%

Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without ischemic preconditioning

Gomes¹,

Filho

Salles

et al. 2011

Acta Cir. Bras.

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. Provided institutional support for the research development. ABSTRACT Purpose:To evaluate the histopathology alterations of the intestinal mucosa of rabbits submitted to mesenteric artery ischemia and reperfusion with and without ischemic preconditioning. Methods: Two groups of ten male New Zealand white rabbits body (weight 2.2-3.0, average 2.5 kg). For mesenteric ischemia induction in all animals the small bowel and mesentery were cut 30cm and 60cm far from the gastroduodenal pyloric transition before the proximal mesenteric artery occlusion. In the Group 1 animals, the proximal mesenteric artery was occluded for 45 min with an atraumatic vascular clamp, followed by reperfusion for 30 min. In the Group 2 the 45 min ischemic phase was preceded by three cycles of ischemia (2 minutes each) alternated with three cycles of reperfusion (2 minutes each). For istopathology study small bowel biopsies were obtained before ischemia (control), after 45 min of mesenteric ischemia and at 30 min. of mesenteric artery reperfusion. Results: In the Group I animals, the followings histopathology grade results were observed: t1, mean 2,8; t2, mean 3,3. Using the Kruskal-Wallis non-parameter test, differences between t0 and t1 and t0 and t2 were significants (p<0.05), but not significant between t1 and t2 (p>0.05). In the Group 2 animals histopathology grade results were: t1 mean 2,6 and t2, mean 2,1. Differences between t0 and t1, t0 and t2 were significant (p<0.05). It was not observed differences (p>0.05) between results of t1 in both groups but histopathology injury observed in Group 1 t2 biopsies were higher (p<0.05) than observed in the same period (t2) of Group 2 animals. Conclusion: Microscopic examination of the biopsies revealed significant evidence of preconditioning protection against small bowel wall ischemia-reperfusion injury. Key words: Mesenteric Vascular Occlusion. Ischemic Preconditioning. Animal Experimentation. Rabbits. RESUMOObjetivo: Avaliar as alterações histopatológicas da mucosa intestinal de coelhos submetidos a isquemia-reperfusão com e sem precondicionamento isquêmicol. Métodos: Foram estudados dois grupos de dez coelhos Nova Zelândia machos com pesos variáveis entre 2,2 e 3,0 kg (média de 2,5 kg) de peso corpóreo. Para indução da isquemia, em todos os animais, o intestino delgado e o mesentério foram seccionados 30 cm e 60 cm após a transição pilórica gastroduodenal, antes da oclusão da artéria mesentérica cranial. Nos animais do Grupo 1, a artéria mesentérica proximal foi ocluida por pinçamento atraumático durante 45 min., seguido de reperfusão por 30 min. No Grupo 2, foi realizado precondicionamento por três ciclos de 2 min. de oclusão mesentérica intercalados com três ciclos de 2 min. de reperfusão, seguido de oclusão mantida por 45 min e reperfusão de 30min. como no Grupo I. Para estudo histopatológico, foram obtidas biópsias da parede intestinal antes da isquemia (t 0 -controle), após 45 min. de isquemia (t 1 ) e após 30 min. de reperfusão (t 2) . Resultados: No Grupo I foram observados os segu...

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Measuring platelet aggregation to estimate small intestinal ischemia-reperfusion injury

Cited by 16 publications

References 24 publications

Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB/NLRP3 Pathway

Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB/NLRP3 Pathway

Novel hematologic inflammatory parameters to predict acute mesenteric ischemia

Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without ischemic preconditioning

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