Measuring cytochrome c redox state using resonance Raman spectroscopy to determine metabolic rates in electron transport chain when exposed to light

Lalonde, Joshua W.; O’Connor, Sean P.; Noojin, Gary D.; Gonzales, Cherry C.; Yakovlev, Vladislav V.; Denton, Michael L.

doi:10.1117/12.2508684

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…RS has been the subject of growing interest in pre-clinical studies for the identification and study of a number of pathophysiological mechanisms. For example, RS has been used to detect oxidative stress-induced inflammation in human endothelial cells [ 25 ], excitotoxicity in epithelial cells [ 26 ], and tracking mitochondrial function [ 27 ]. Raman techniques have been utilised in alternative brain injury models, demonstrating consistent RS spectral changes in response to injury, including rat ischaemia–reperfusion injury [ 28 ], biochemical change in post-traumatic stress disorder rat models [ 29 ], and radiation damage to the brain in mice [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Raman Spectroscopy as a Neuromonitoring Tool in Traumatic Brain Injury: A Systematic Review and Clinical Perspectives

Stevens

Stickland

Harris

et al. 2022

Cells

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Traumatic brain injury (TBI) is a significant global health problem, for which no disease-modifying therapeutics are currently available to improve survival and outcomes. Current neuromonitoring modalities are unable to reflect the complex and changing pathophysiological processes of the acute changes that occur after TBI. Raman spectroscopy (RS) is a powerful, label-free, optical tool which can provide detailed biochemical data in vivo. A systematic review of the literature is presented of available evidence for the use of RS in TBI. Seven research studies met the inclusion/exclusion criteria with all studies being performed in pre-clinical models. None of the studies reported the in vivo application of RS, with spectral acquisition performed ex vivo and one performed in vitro. Four further studies were included that related to the use of RS in analogous brain injury models, and a further five utilised RS in ex vivo biofluid studies for diagnosis or monitoring of TBI. RS is identified as a potential means to identify injury severity and metabolic dysfunction which may hold translational value. In relation to the available evidence, the translational potentials and barriers are discussed. This systematic review supports the further translational development of RS in TBI to fully ascertain its potential for enhancing patient care.

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Section: Introductionmentioning

confidence: 99%

Raman Spectroscopy as a Neuromonitoring Tool in Traumatic Brain Injury: A Systematic Review and Clinical Perspectives

Stevens

Stickland

Harris

et al. 2022

Cells

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Photon absorption in the mitochondria: Potential immediate and early events associated with photobiomodulation

Denton

Gonzalez

Noojin

et al. 2019

Mechanisms of Photobiomodulation Therapy XIV

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Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic “signature”

et al. 2022

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Apoptotic cell death within the brain represents a significant contributing factor to impaired post-traumatic tissue function and poor clinical outcome after traumatic brain injury. After irradiation with light in the wavelength range of 600–1200 nm (photobiomodulation), previous investigations have reported a reduction in apoptosis in various tissues. This study investigates the effect of 660 nm photobiomodulation on organotypic slice cultured hippocampal tissue of rats, examining the effect on apoptotic cell loss. Tissue optical Raman spectroscopic changes were evaluated. A significantly higher proportion of apoptotic cells 62.8±12.2% vs 48.6±13.7% (P<0.0001) per region were observed in the control group compared with the photobiomodulation group. After photobiomodulation, Raman spectroscopic observations demonstrated 1440/1660 cm-1 spectral shift. Photobiomodulation has the potential for therapeutic utility, reducing cell loss to apoptosis in injured neurological tissue, as demonstrated in this in vitro model. A clear Raman spectroscopic signal was observed after apparent optimal irradiation, potentially integrable into therapeutic light delivery apparatus for real-time dose metering.

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Measuring cytochrome c redox state using resonance Raman spectroscopy to determine metabolic rates in electron transport chain when exposed to light

Cited by 3 publications

References 8 publications

Raman Spectroscopy as a Neuromonitoring Tool in Traumatic Brain Injury: A Systematic Review and Clinical Perspectives

Raman Spectroscopy as a Neuromonitoring Tool in Traumatic Brain Injury: A Systematic Review and Clinical Perspectives

Photon absorption in the mitochondria: Potential immediate and early events associated with photobiomodulation

Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic “signature”

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