Measuring antimicrobial susceptibility of Pseudomonas aeruginosa using Poloxamer 407 gel

Yamada, Hiroyuki; Koike, Naohito; Ehara, Tomoko; Matsumoto, Tetsuya

doi:10.1007/s10156-010-0109-x

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…19,20 Moreover, poloxamer hydrogels were used to mimic biofilm environment for assessment of confined bacteria susceptibility towards antimicrobial treatments, thus remarking its harmlessness in these experimental conditions. [21][22][23] In this work, in contrast, we described that Pluronic nanopolymers possess a broadspectrum bactericidal activity, and we illustrated the dramatic influence of nanopolymer superstructuration in the bactericidal performance. The synthesis and assessment of mesostructured titania thin films synthesized by the sol-gel method combined with evaporation-induced self-assembly of Pluronic micelles was described.…”

Section: Introductionmentioning

confidence: 84%

Transforming an inert nanopolymer into broad-spectrum bactericidal by superstructure tuning

Scilletta

Pezzoni

Desimone

et al. 2019

Colloids and Surfaces B: Biointerfaces

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Highlights Nano-engineered hybrid Pluronic-titania films show bactericidal activity  Superstructuration can turn inert self-assembled polymer into highly bactericidal  Maximum bactericidal efficiency relies on appropriate superstructured configuration  A hybrid biocompatible coating with broad-spectrum antimicrobial activity is shown

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Section: Introductionmentioning

confidence: 84%

Transforming an inert nanopolymer into broad-spectrum bactericidal by superstructure tuning

Scilletta

Pezzoni

Desimone

et al. 2019

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

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“…The same study also showed that biofilm formation related to growth on poloxamer 407 gel. 83 with Live/Dead bacterial staining were elsewhere applied for antibiofilm testing. 84 Such an effect could not be observed in our study, presumably being overcompensated by other SLN components.…”

Section: Effect Of Slns On Growth Of Planktonic P Aeruginosa Isolatesmentioning

confidence: 99%

Enzyme-Linked Lipid Nanocarriers for Coping Pseudomonal Pulmonary Infection. Would Nanocarriers Complement Biofilm Disruption or Pave Its Road?

Nafee,

Gaber,

Abouelfetouh

et al. 2024

IJN

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Introduction: Cystic fibrosis (CF) is associated with pulmonary Pseudomonas aeruginosa infections persistent to antibiotics. Methods: To eradicate pseudomonal biofilms, solid lipid nanoparticles (SLNs) loaded with quorum-sensing-inhibitor (QSI, disrupting bacterial crosstalk), coated with chitosan (CS, improving internalization) and immobilized with alginate lyase (AL, destroying alginate biofilms) were developed. Results: SLNs (140-205 nm) showed prolonged release of QSI with no sign of acute toxicity to A549 and Calu-3 cells. The CS coating improved uptake, whereas immobilized-AL ensured >1.5-fold higher uptake and doubled SLN diffusion across the artificial biofilm sputum model. Respirable microparticles comprising SLNs in carbohydrate matrix elicited aerodynamic diameters MMAD (3.54, 2.48 µm) and fine-particle-fraction FPF (65, 48%) for anionic and cationic SLNs, respectively. The antimicrobial and/or antibiofilm activity of SLNs was explored in Pseudomonas aeruginosa reference mucoid/nonmucoid strains as well as clinical isolates. The full growth inhibition of planktonic bacteria was dependent on SLN type, concentration, growth medium, and strain. OD measurements and live/dead staining proved that anionic SLNs efficiently ceased biofilm formation and eradicated established biofilms, whereas cationic SLNs unexpectedly promoted biofilm progression. AL immobilization increased biofilm vulnerability; instead, CS coating increased biofilm formation confirmed by 3D-time lapse confocal imaging. Incubation of SLNs with mature biofilms of P. aeruginosa isolates increased biofilm density by an average of 1.5-fold. CLSM further confirmed the binding and uptake of the labeled SLNs in P. aeruginosa biofilms. Considerable uptake of CS-coated SLNs in non-mucoid strains could be observed presumably due to interaction of chitosan with LPS glycolipids in the outer cell membrane of P. aeruginosa. Conclusion:The biofilm-destructive potential of QSI/SLNs/AL inhalation is promising for site-specific biofilm-targeted interventional CF therapy. Nevertheless, the intrinsic/extrinsic fundamentals of nanocarrier-biofilm interactions require further investigation.

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“…4A, there is a small effect of shellac NPs on the algal cells at relatively high concentration due to the antibacterial properties of P407, which was used as a stabilizer. It has been previously reported that P407 might create a adsorption layer on the microbial cell surface, [55][56][57] so the combination between P407 and the shellac NPs may play a synergistic antimicrobial role within this nanocarrier design. The shellac NPs, however, showed no pronounced effect when incubated with yeast and E. coli for 6 hours, as demonstrated in Fig.…”

Section: The Cytotoxicity Effect Of Non-loaded Shellac Npsmentioning

confidence: 99%

Dual-functionalised shellac nanocarriers give a super-boost of the antimicrobial action of berberine

2019

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Measuring antimicrobial susceptibility of Pseudomonas aeruginosa using Poloxamer 407 gel

Cited by 6 publications

References 16 publications

Transforming an inert nanopolymer into broad-spectrum bactericidal by superstructure tuning

Transforming an inert nanopolymer into broad-spectrum bactericidal by superstructure tuning

Enzyme-Linked Lipid Nanocarriers for Coping Pseudomonal Pulmonary Infection. Would Nanocarriers Complement Biofilm Disruption or Pave Its Road?

Dual-functionalised shellac nanocarriers give a super-boost of the antimicrobial action of berberine

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