Measuring Antigen‐Specific Immune Responses, 2008 update

Gratama, Jan-Willem; Kern, Florian; Manca, Fabrizio

doi:10.1002/cyto.a.20655

Cited by 11 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To determine the cytotoxicity of CD8 + T cells the human fluorescent antigen-transfected target cell (FATT)-CTL assay was adapted for use in sheep to identify the epitopes that induce CTL for inclusion in a multiepitope vaccine. The FATT-CTL assay combines non-radioactive quantification of target cell killing with an efficient plasmid transfection technology to achieve antigen expression in target cells [18]. It measures antigen-specific cell mediated cytotoxicity and is a convenient alternative to the classic radioactive Cr 51 -release assay.…”

Section: Introductionmentioning

confidence: 99%

Ehrlichia ruminantium antigens and peptides induce cytotoxic T cell responses in vitro

Thema

Tshilwane

Sơn

et al. 2019

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

Since CD8 + T cells play an important role in resistance to infection with heartwater, effective vaccines against this disease will likely require identification of antigens that contain CD8 + T cell epitopes responsible for cytotoxic T lymphocyte (CTL) responses. With the use of the fluorescent antigen-transfected target cell (FATT)-CTL assay, IFN-γ ELISPOT and flow cytometry, peptides that induce CTL, proliferation of CD8+T cells and IFN-γ production were identified as possible target antigens for vaccine development. Of particular relevance was the finding that different peptides from different antigens were able to elicit varied cytotoxic activities by immune peripheral blood mononuclear cells (PBMC) from heartwater immune tick-infected sheep. Several peptides derived from Erum0660, Erum2330, Erum2540, Erum2580 and Erum5000 induced CTL in immune sheep PBMC. Peptide Erum2540-6 was the only peptide that induced significant CTL, CD8 + CD45RO + and CD8 + IFN-γ + by PBMC from all three sheep, and Erum2540 and p2540-20 induced the highest % CTL response in all three outbred sheep. These results suggest that these epitopes may be of major importance in heartwater recombinant vaccine development.

show abstract

Section: Introductionmentioning

confidence: 99%

Ehrlichia ruminantium antigens and peptides induce cytotoxic T cell responses in vitro

Thema

Tshilwane

Sơn

et al. 2019

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

show abstract

“…It is also needed in the course of immunotherapy studies, with the aim to establish immunological correlates of clinical efficacy. Various functional assays conducted following allergen stimulation are available to analyse specific CD4 + T cells, such as proliferation assessment, intracellular cytokine staining, cytokine secretion assay (CSA) and ELISPOT [4, 5]. However, immunodetection using major histocompatibility complex (MHC) class II/peptide tetramers (tetramers) is currently considered as the most specific approach in order to identify allergen‐reactive CD4 + T cells [6–8].…”

Section: Introductionmentioning

confidence: 99%

Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen‐specific CD4⁺ T cells

Bonvalet

Wambre

Moussu

et al. 2011

Clin Experimental Allergy

View full text Add to dashboard Cite

No strict correlation exists between tetramer staining and the expression of multiple activation markers in stimulated CD4(+) T cells. Dual staining allows to discriminate functional tetramer(+) /marker(+) vs. anergic (tetramer(+) /marker(-) ) allergen-specific T cells or non-specifically activated (tetramer(-) /marker(+) ) T cells. Combining tetramer staining with the detection of activation markers helps understanding patient heterogeneity regarding specific CD4(+) T cell responses. This approach has immediate relevance for monitoring immune changes induced during specific immunotherapy.

show abstract

“…T cell response to a specific antigen is analyzed by several immune assays including direct multimer staining, ELISPOT, intracellular cytokine staining, and proliferation assays, based on detection following stimulation of the T cells in vitro with antigen [ 59 , 60 ]. However, many of these methods suffer from low sensitivity, and they show a significant variability in the quantitative readouts [ 61 , 62 , 63 ].…”

Section: Systems Vaccinology For Cancer Vaccines Applicationmentioning

confidence: 99%

Systems Biology Approach for Cancer Vaccine Development and Evaluation

et al. 2015

View full text Add to dashboard Cite

Therapeutic cancer vaccines do not hold promise yet as an effective anti-cancer treatment. Lack of efficacy or poor clinical outcomes are due to several antigenic and immunological aspects that need to be addressed in order to reverse such trends and significantly improve cancer vaccines’ efficacy. The newly developed high throughput technologies and computational tools are instrumental to this aim allowing the identification of more specific antigens and the comprehensive analysis of the innate and adaptive immunities. Here, we review the potentiality of systems biology in providing novel insights in the mechanisms of the action of vaccines to improve their design and effectiveness.

show abstract

Measuring Antigen‐Specific Immune Responses, 2008 update

Cited by 11 publications

References 20 publications

Ehrlichia ruminantium antigens and peptides induce cytotoxic T cell responses in vitro

Ehrlichia ruminantium antigens and peptides induce cytotoxic T cell responses in vitro

Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen‐specific CD4⁺ T cells

Systems Biology Approach for Cancer Vaccine Development and Evaluation

Contact Info

Product

Resources

About

Measuring Antigen‐Specific Immune Responses, 2008 update

Cited by 11 publications

References 20 publications

Ehrlichia ruminantium antigens and peptides induce cytotoxic T cell responses in vitro

Ehrlichia ruminantium antigens and peptides induce cytotoxic T cell responses in vitro

Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen‐specific CD4+ T cells

Systems Biology Approach for Cancer Vaccine Development and Evaluation

Contact Info

Product

Resources

About

Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen‐specific CD4⁺ T cells