Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen‐specific CD4<sup>+</sup> T cells

Bonvalet, Mélodie; Wambre, Erik; Moussu, Hélène; Horiot, S.; Kwok, William W.; Louise, Anne; Ebo, Didier G.; Hoarau, C.; Overtvelt, van L; Baron‐Bodo, Véronique; Moingeon, Philippe

doi:10.1111/j.1365-2222.2011.03708.x

Cited by 25 publications

(26 citation statements)

References 43 publications

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“…One of the novel aspects of our analysis is the use of peptide:MHC class II tetramers to define the allergen-specific CD4 + T cell population recruited into the airways in response to allergen (35, 36). Using this technology, we found a marked increase in the numbers of allergen-specific CD4 + T cells in the BAL twenty-four hours following allergen challenge in both groups, suggesting recruitment of these cells into the airway.…”

Section: Discussionmentioning

confidence: 99%

Allergic asthma is distinguished by sensitivity of allergen-specific CD4 ⁺ T cells and airway structural cells to type 2 inflammation

et al. 2016

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Despite systemic sensitization, not all allergic individuals develop asthma symptoms upon airborne allergen exposure. Determination of the factors that lead to the asthma phenotype in allergic individuals could guide treatment and identify novel therapeutic targets. In this study, we utilized segmental allergen challenge (SAC) of allergic asthmatics (AA) and allergic non-asthmatic controls (AC) to determine if there are differences in the airway immune response or airway structural cells that could drive the development of asthma. Both groups developed prominent allergic airway inflammation in response to allergen. However, asthmatic subjects had markedly higher levels of innate type 2 receptors on allergen-specific CD4+ T cells recruited into the airway. There were also increased levels of type 2 cytokines, increased total mucin and increased MUC5AC in response to allergen in the airways of AA subjects. Furthermore, type 2 cytokine levels correlated with the mucin response in AA but not AC subjects, suggesting differences in the airway epithelial response to inflammation. Finally, AA subjects had increased airway smooth muscle mass at baseline measured in vivo using novel orientation-registered optical coherence tomography (OR-OCT). Our data demonstrate that the development of allergic asthma is dependent on the responsiveness of allergen-specific CD4+ T cells to innate type 2 mediators as well as increased sensitivity of airway epithelial cells and smooth muscle to type 2 inflammation.

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Section: Discussionmentioning

confidence: 99%

Allergic asthma is distinguished by sensitivity of allergen-specific CD4 ⁺ T cells and airway structural cells to type 2 inflammation

et al. 2016

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“…A previous study with CD154/ tetramer co-staining after prolonged cell culture and re-stimulation questioned the validity of CD154 as a marker for allergen-specific T cells [10], but these experimental conditions are known to invalidate the assay, which is only reliable after short-term stimulation [6]. The close relationship between Th1, Th2 and Tr1-like cells was first reported by Akdis et al [2] using cytokine capture technology.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of CD154+ T cells following ex vivo birch allergen stimulation defines a close relationship between T cell subsets in healthy volunteers

et al. 2013

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BackgroundAllergic sensitisation has been ascribed to a dysregulated relationship between allergen-specific Th1, Th2 and regulatory T cells. We hypothesised that the relationship between these T cell subsets could be better defined using a short-term allergen stimulation system followed by direct analysis of CD154-positive T cells. Using peripheral blood samples from birch pollinosis patients and healthy non-atopic controls, we sought to explore the frequencies and phenotype of birch-stimulated CD154-positive T helper cells following ex vivo birch allergen stimulation.ResultsActivated CD154-positive Th1, Th2 and Tr1-like cells, that co-expressed IFNγ, IL-4 and IL-10 respectively, were identified in both birch-allergic and non-allergic participants. We observed a close correlation between Th1, Th2 and Tr1-like cell frequency in non-allergic volunteers, such that the three parameters increased together to maintain a low Th2: Th1 ratio. The relationship between Th1, Th2 and Tr1-like responses was dysregulated in birch-allergic patients, with abrogation of the IL-10 response and a higher Th2: Th1 ratio. A close correlation was observed between Th2 cell frequency and the absolute concentration of birch-specific IgE within the birch-allergic group, and we confirmed previous reports of a more differentiated T cell phenotype in allergic subjects.ConclusionsThe findings demonstrate an important balance between IFNγ, IL-4 and IL-10 T cell responses to birch allergen in health, where Th2 responses to allergens were frequently observed, but apparently balanced by Th1 and regulatory responses. The detection of CD154 positive T cells after short-term antigen stimulation may be a useful method for the detection of T cell responses to allergens when cost, speed and convenience are priorities.

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“…For this reason, Wambre and colleagues have used T cell surface/intracellular marker expression with or without associated tetramer staining to characterize subjects allergic to grass pollen and mites, with the aim of distinguishing antigen-specific T cells from bystander T cell responses [29]. These and other studies [30] have identified CD154 (CD40 ligand) as a potentially useful T cell marker for future immunotherapy studies.…”

Section: Chapter 3 Mechanisms Of Sublingual Immunotherapymentioning

confidence: 99%

Sublingual immunotherapy: World Allergy Organization position paper 2013 update

Canonica

Cox

Pawankar

et al. 2014

World Allergy Organization Journal

447

421

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We have prepared this document, “Sublingual Immunotherapy: World Allergy Organization Position Paper 2013 Update”, according to the evidence-based criteria, revising and updating chapters of the originally published paper, “Sublingual Immunotherapy: World Allergy Organization Position Paper 2009”, available at http://www.waojournal.org. Namely, these comprise: “Mechanisms of sublingual immunotherapy;” “Clinical efficacy of sublingual immunotherapy” – reporting all the data of all controlled trials published after 2009; “Safety of sublingual immunotherapy” – with the recently published Grading System for adverse reactions; “Impact of sublingual immunotherapy on the natural history of respiratory allergy” – with the relevant evidences published since 2009; “Efficacy of SLIT in children” – with detailed analysis of all the studies; “Definition of SLIT patient selection” – reporting the criteria for eligibility to sublingual immunotherapy; “The future of immunotherapy in the community care setting”; “Methodology of clinical trials according to the current scientific and regulatory standards”; and “Guideline development: from evidence-based medicine to patients' views” – including the evolution of the methods to make clinical recommendations.Additionally, we have added new chapters to cover a few emerging crucial topics: “Practical aspects of schedules and dosages and counseling for adherence” – which is crucial in clinical practice for all treatments; “Perspectives and new approaches” – including recombinant allergens, adjuvants, modified allergens, and the concept of validity of the single products. Furthermore, “Raising public awareness about sublingual immunotherapy”, as a need for our patients, and strategies to increase awareness of allergen immunotherapy (AIT) among patients, the medical community, all healthcare stakeholders, and public opinion, are also reported in detail.

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Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen‐specific CD4⁺ T cells

Cited by 25 publications

References 43 publications

Allergic asthma is distinguished by sensitivity of allergen-specific CD4 ⁺ T cells and airway structural cells to type 2 inflammation

Allergic asthma is distinguished by sensitivity of allergen-specific CD4 ⁺ T cells and airway structural cells to type 2 inflammation

Characterisation of CD154+ T cells following ex vivo birch allergen stimulation defines a close relationship between T cell subsets in healthy volunteers

Sublingual immunotherapy: World Allergy Organization position paper 2013 update

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Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen‐specific CD4+ T cells

Cited by 25 publications

References 43 publications

Allergic asthma is distinguished by sensitivity of allergen-specific CD4 + T cells and airway structural cells to type 2 inflammation

Allergic asthma is distinguished by sensitivity of allergen-specific CD4 + T cells and airway structural cells to type 2 inflammation

Characterisation of CD154+ T cells following ex vivo birch allergen stimulation defines a close relationship between T cell subsets in healthy volunteers

Sublingual immunotherapy: World Allergy Organization position paper 2013 update

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Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen‐specific CD4⁺ T cells

Allergic asthma is distinguished by sensitivity of allergen-specific CD4 ⁺ T cells and airway structural cells to type 2 inflammation

Allergic asthma is distinguished by sensitivity of allergen-specific CD4 ⁺ T cells and airway structural cells to type 2 inflammation