Measurements of electron transport activities in marine phytoplankton

Kenner, R. A.; Ahmed, S. I.

doi:10.1007/bf00390716

Cited by 291 publications

(176 citation statements)

References 22 publications

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“…The respiratory potential of mitochondria was measured in plants via terminal electron transport system (ETS) activity as described by Packard (1971) and Kenner and Ahmed (1975). Plant material was homogenised in an ice-cold homogenisation buffer and sonicated with an ultrasound homogeniser (40W, 4710, Cole-Parmer, Vernon Hills, IL, USA).…”

Section: Ets Activitymentioning

confidence: 99%

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Selenium induced lower respiratory potential in Glycine max (L.) Merr

Mechora¹,

Germ²

2010

Acta Agriculturae Slovenica

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Effect of selenium (Se) was studied in soybean (Glycine max (L.) Merr.) cv. Olna in Ljubljana, Slovenia. Se was added to plants as foliary spraying. Quantum yield of photosystem II (PSII) and respiratory potential measured as ETS activity of mitochondria were measured two times in the growing season. Respiratory potential was higher in young plants compared to mature plants. Se induced the lowering of respiratory potential. Addition of Se had no effect on quantum yield of photosystem II.

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Section: Ets Activitymentioning

confidence: 99%

“…After stopping the reaction with a stopping solution (formaldehyde and phosphoric acid, 1:1), the formazan absorption at 490 nm was determined. ETS activity was measured as the rate of tetrazolium dye reduction, and converted to equivalent oxygen as described by Kenner and Ahmed (1975).…”

Section: Ets Activitymentioning

confidence: 99%

Selenium induced lower respiratory potential in Glycine max (L.) Merr

Mechora¹,

Germ²

2010

Acta Agriculturae Slovenica

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“…Measurement of terminal electron transport system activity Respiratory potential was estimated via the terminal electron transport system (ETS) activity of mitochondria as described by Packard (1971) and modified by Kenner & Ahmed (1975). Algae were homogenized in cold buffer (0 INT was used as the electron acceptor, being reduced to formazan, whose absorbance is measured at 490 nm.…”

Section: Fluorescence Measurementsmentioning

confidence: 99%

UV-B radiation and selenium affected energy availability in green alga Zygnema

2009

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Green alga Zygnema was exposed to three concentrations of selenium and two levels of UV-B radiation. The combined effects of both treatments on energy availability; photochemical quantum yield and respiratory potential were studied. Our findings show that traces of selenium enhance metabolic process connected with photochemical quantum yield and mitochondrial respiration. Surprisingly, selenium does not diminish the effects of UV-B radiation; on the contrary, the combined action of UV-B radiation and traces of selenium leads to pronounced negative effects on photochemical quantum yield and the respiratory potential. Selenium is involved in the activation of energy resources in green alga Zygnema. The importance of selenium for activity of the mitochondria is possibly an evolutionary recollection from an endosymbiotic bacterium.

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“…In set 2, an automatic sample pretreatment injection line system with a robotic arm (ASPEC-Gilson 221/222) was used to carry out the essays. ETS activity was calculated from the absorbance of the INTformazan production rate in the ETS assay and from the formazan molar extinction coefficient (Kenner and Ahmed, 1975;Oxtoby et al, 1999) according to Gómez et al (1996). ETS activity measured in this manner is consistent with the cytochrome c reduction ETS assay of Borgmann (1977).…”

Section: Ets Activity and Potential Respirationmentioning

confidence: 88%

Potential respiration is a better respiratory predictor than biomass in young Artemia salina

Martínez

Packard

2010

Journal of Experimental Marine Biology and Ecology

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These experiments test whether respiration can be predicted better from biomass or from potential respiration, a measurement of the mitochondrial and microsomal respiratory electron transport systems. For nearly a century Kleiber's law or a similar precursor have argued the importance of biomass in predicting respiration. In the last decade, a version of the Metabolic Theory of Ecology has elaborated on Kleiber's Law adding emphasis to the importance of biomass in predicting respiration. We argue that Kleiber's law works because biomass packages mitochondria and microsomal electron transport complexes. On a scale of five orders of magnitude we have shown previously that potential respiration predicts respiration as well as biomass in marine zooplankton. Here, using cultures of the branchiopod, Artemia salina and on a scale of less than 2 orders of magnitude, we investigated the power of biomass and potential respiration in predicting respiration. We measured biomass, respiration and potential respiration in Artemia grown in different ways and found that potential respiration (Ф) could predict respiration (R), both in µlO 2 h −1 (R =0.924Φ +0.062, r 2 = 0.976), but biomass (as mg dry mass) could not (R = 27.02DM + 8.857, r 2 = 0.128). Furthermore the R/Ф ratio appeared independent of age and differences in the food source.

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Measurements of electron transport activities in marine phytoplankton

Cited by 291 publications

References 22 publications

Selenium induced lower respiratory potential in Glycine max (L.) Merr

Selenium induced lower respiratory potential in Glycine max (L.) Merr

UV-B radiation and selenium affected energy availability in green alga Zygnema

Potential respiration is a better respiratory predictor than biomass in young Artemia salina

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