Measurements of deoxyribosenucleic acid (DNA) in higher plants by Feulgen photometry and chemical methods

McLeish, John; Sunderland, N.

doi:10.1016/0014-4827(61)90452-9

Cited by 180 publications

(49 citation statements)

References 23 publications

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“…Staining was done in SchifT's reagent for I h and excess stain was washed oil with three rinses in aq. 10 per cent potassium metabisuiphite solution (McLeish and Sunderland, 1961).…”

Section: Methodsmentioning

confidence: 99%

Inter and intraspecific variation in nuclear DNA content in Aedes mosquitoes

Rao

1987

Heredity

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Haploid nuclear DNA of 23 species of Aedes, as determined by Feulgen cytophotometry, was found to vary 3-fold. This was accompanied by a 2-fold variation in total chromosomal length. There was a significant correlation (r = 9765, P< 0.001) between these two parameters. Genome size varied from 0-87 pg to 1-3 pg among 10 strains of Aedes albopictus, from wide geographic regions. Large scale differences in chromosomal DNA amounts have accompanied speclation and evolution in aedine mosquitoes.

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“…Staining was done in SchifT's reagent for I h and excess stain was washed oil with three rinses in aq. 10 per cent potassium metabisuiphite solution (McLeish and Sunderland, 1961).…”

Section: Methodsmentioning

confidence: 99%

Inter and intraspecific variation in nuclear DNA content in Aedes mosquitoes

Rao

1987

Heredity

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“…Feulgen photometry is now extensively used to estimate relative amounts of DNA in individual nuclei (Leutcheriberger, 1954;Swift, 1955;Vendrely and Vendrely, 1956;Waker, 1959;McLeish, 1961). The validity of this method has been well established by comparisons between estimations of Feulgen stain values with chemically determined estimates of DNA in the same material (Deeley, Davies and Chayen, 1957;Ris and Mirsky, 1949;Leuchtenberger, 1954;McLeish and Sunderland, 1961).…”

Section: Experimental Techniques (A) Feulgen Photometrymentioning

confidence: 99%

Nuclear changes in flax

Evans

1968

Heredity

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“…To estimate Dna content per nucleus, sections were stained by the Feulgen method substituting 1N Trichloroacetic acid (TCA) for 1N HCl in both the hydrolysis and the Schiff reagent. Measurements of Dna content in individual nuclei were made on 3 longitudinal median serial sections of five root tips ( 100 cells in each root tips), following the procedure of McLEISH and SuNDERLAND (1961). The nuclei, after being scanned for Feulgen Dna, were scanned for amount of alkaline Fast-green stainable histones according to the method of ALPERT and GESCHWIND (1953).…”

Section: Methodsmentioning

confidence: 99%