Inter and intraspecific variation in nuclear DNA content in Aedes mosquitoes

Rao, P. Nagesh; S, K

doi:10.1038/hdy.1987.120

Cited by 70 publications

(41 citation statements)

References 31 publications

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“…albopictus is equal to or greater than that of Ae. aegypti (Rao & Rai, 1987) and their chromosome 1 morphologies are similar (Motara & Rai, 1978), the basis for the differences in recombination frequencies is unclear. A possible influence is the variability in nuclear DNA content among Ae.…”

Section: Resultsmentioning

confidence: 99%

Comparative linkage maps for the mosquitoes, Aedes albopictus and Ae. aegypti, based on common RFLP loci

Severson

Mori

Kassner

et al. 1995

Insect Molecular Biology

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Aedes albopictus and Aedes aegypfi are members of the mosquito family Culicidae and share a haploid chromosome complement of three. Although a genetic linkage map based on restriction fragment length polymorphism (RFLP), markers exists for Ae. aegypti, the extent of synteny and linkage order conservation between the two species was unknown. A comparative linkage map for Ae. albopictus was constructed based mainly on cDNA clones from Ae. aegypfi. Nearly all Ae. aegypti probes hybridized to Ae. albopictus DNA at high stringency. For eighteen RFLP markers tested, the linkage group and linear order appears to be identical for the two species. 78% of the loci tested exhibited significant deviations from the expected segregation ratio in at least one of the test crosses. An excess of heterozygote genotypes was recovered with most loci. This probably reflects the effects of lethal loci on survival of F2 progeny homozygous for the parental genotypes. These results demonstrate that comparative linkage maps based on common DNA markers provide a basis for rapidly developing linkage maps for various mosquito species, and the opportunity to examine the significance and function of orthologous quantitative trait loci associated with mosquito vector competence for disease transmission.

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Section: Resultsmentioning

confidence: 99%

Comparative linkage maps for the mosquitoes, Aedes albopictus and Ae. aegypti, based on common RFLP loci

Severson

Mori

Kassner

et al. 1995

Insect Molecular Biology

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“…Similarly, highly repetitive MITE families have been found in Ae. aegypti, which has a relatively large genome (800 Mbp) with a high level of repetitive sequences (27,40). In contrast, no MITEs have been reported in the most extensively studied insect, D. melanogaster, which has a small genome (130-140 Mbp) and a low level of repetitive sequences (41)(42)(43).…”

Section: Mite-like Elements and The Size And Organization Of Eukaryotmentioning

confidence: 95%

Three novel families of miniature inverted-repeat transposable elements are associated with genes of the yellow fever mosquito,Aedes aegypti

1997

Proc. Natl. Acad. Sci. U.S.A.

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Three novel families of transposable elements, Wukong, Wujin, and Wuneng, are described in the yellow fever mosquito, Aedes aegypti. Their copy numbers range from 2,100 to 3,000 per haploid genome. There are high degrees of sequence similarity within each family, and many structural but not sequence similarities between families. The common structural characteristics include small size, no coding potential, terminal inverted repeats, potential to form a stable secondary structure, A؉T richness, and putative 2-to 4-bp A؉T-biased specific target sites. Evidence of previous mobility is presented for the Wukong elements. Elements of these three families are associated with 7 of 16 fully or partially sequenced Ae. aegypti genes. Characteristics of these mosquito elements indicate strong similarities to the miniature inverted-repeat transposable elements (MITEs) recently found to be associated with plant genes. MITE-like elements have also been reported in two species of Xenopus and in Homo sapiens. This characterization of multiple families of highly repetitive MITE-like elements in an invertebrate extends the range of these elements in eukaryotic genomes. A hypothesis is presented relating genome size and organization to the presence of highly reiterated MITE families. The association of MITElike elements with Ae. aegypti genes shows the same bias toward noncoding regions as in plants. This association has potentially important implications for the evolution of gene regulation.

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“…tromeric C-bands. There are many reports of interspecific genome size differences due to the amount of highly repeated DNA in animals (Rees et al, 1976;Rao & Rai, 1987;Black & Rai, 1988). A direct correlation between total DNA content and C-heterochromatic DNA is a general rule for primate species (Manfredi Romanini et al, 1991;Ronchetti et al, 1993): talitroidean amphipods may represent a further example.…”

Section: General Remarksmentioning

confidence: 99%

Chromosome number, karyotype morphology, heterochromatin distribution and nuclear DNA content of some talitroidean amphipods (Crustacea: Gammaridea)

Libertini¹,

Trisolini²,

Rampin³

2008

Eur. J. Entomol.

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Abstract. Chromosome number, karyotype formula, C-banding pattern, genome size and DNA base composition were studied in three species of Hyalidae and seven species of Talitridae. A karyotype of 25 chromosome pairs, with median centromeres (FN = 100), was found in all the species of Talitridae analysed and Apohyale prevostii. Genome size (C-value) varies among Talitrida from 0.94 pg in Apohyale crassipes to 2.81 pg in Orchestia gammarellus, and the percentage of AT-DNA in the whole genome ranges from 56.12% in A. crassipes to 68.17% in Sardorchestia pelecaniformis. In comparison with Hyalidae, Talitridae show more uniformity in chromosome number and karyotype formula, and have larger genomes. There is a direct correlation between total DNA content and the amount of C-heterochromatic DNA. The cytogenetical data on Talitrida were compared from a phylogenetic and an evolutional point of view. The increase in genome size during the evolution of the Talitrida possibly had a role in their adaptation to supralittoral life and extreme subaerial conditions.

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Inter and intraspecific variation in nuclear DNA content in Aedes mosquitoes

Cited by 70 publications

References 31 publications

Comparative linkage maps for the mosquitoes, Aedes albopictus and Ae. aegypti, based on common RFLP loci

Comparative linkage maps for the mosquitoes, Aedes albopictus and Ae. aegypti, based on common RFLP loci

Three novel families of miniature inverted-repeat transposable elements are associated with genes of the yellow fever mosquito,Aedes aegypti

Chromosome number, karyotype morphology, heterochromatin distribution and nuclear DNA content of some talitroidean amphipods (Crustacea: Gammaridea)

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