Measurement Techniques for Red Blood Cell Deformability: Recent Advances

Kim, Young-Chan; K, Kim; Park, YongKeun

doi:10.5772/50698

Cited by 72 publications

(111 citation statements)

References 135 publications

(112 reference statements)

Supporting

Mentioning

109

Contrasting

Order By: Relevance

“…An RBC was modeled as a shell (that is, cell membrane thickness: 10 nm, Young's modulus: 1 kPa (Ref. 26)) encapsulating fluids (that is, hemoglobin which is incompressible 27 ). It was modeled as a biconcave disk (diameter: 8 μm) with a thickness at the thickest point of 2 μm and a minimum thickness in the center of 1 μm.…”

Section: Methodsmentioning

confidence: 99%

Stiffness increase of red blood cells during storage

Zheng

Wang

et al. 2018

Microsyst Nanoeng

View full text Add to dashboard Cite

In transfusion medicine, the deformability of stored red blood cells (RBCs) changes during storage in blood banks. Compromised RBC deformability can reduce the transfusion efficiency or intensify transfusion complications, such as sepsis. This paper reports the microfluidic mechanical measurement of stored RBCs under the physiological deformation mode (that is, folding). Instead of using phenomenological metrics of deformation or elongation indices (DI or EI), the effective stiffness of RBCs, a flow velocityindependent parameter, is defined and used for the first time to evaluate the mechanical degradation of RBCs during storage. Fresh RBCs and RBCs stored up to 6 weeks (42 days) in the blood bank were measured, revealing that the effective stiffness of RBCs increases over the storage process. RBCs stored for 1 week started to show significantly higher stiffness than fresh RBCs, and stored RBC stiffness degraded faster during the last 3 weeks than during the first 3 weeks. Furthermore, the results indicate that the time points of the effective stiffness increase coincide well with the degradation patterns of S-nitrosothiols (SNO) and adenosine triphosphate (ATP) in RBC storage lesions.

show abstract

Section: Methodsmentioning

confidence: 99%

Stiffness increase of red blood cells during storage

Zheng

Wang

et al. 2018

Microsyst Nanoeng

View full text Add to dashboard Cite

show abstract

“…Therefore, a physically-based model would be preferable. From a mechanical viewpoint, the RBC is a bag filled with hemoglobin, its shape is mostly determined by the minimum of the membrane free energy [17]. It was assumed [18] that the main shape-dependent part of the latter is the membrane bending energy.…”

Section: Introductionmentioning

confidence: 99%

Mature red blood cells: from optical model to inverse light-scattering problem

Gilev¹,

Yurkin²,

Chernyshova³

et al. 2016

Biomed. Opt. Express

View full text Add to dashboard Cite

Abstract:We propose a method for characterization of mature red blood cells (RBCs) morphology, based on measurement of light-scattering patterns (LSPs) of individual RBCs with the scanning flow cytometer and on solution of the inverse light-scattering (ILS) problem for each LSP. We considered a RBC shape model, corresponding to the minimal bending energy of the membrane with isotropic elasticity, and constructed an analytical approximation, which allows rapid simulation of the shape, given the diameter and minimal and maximal thicknesses. The ILS problem was solved by the nearest-neighbor interpolation using a preliminary calculated database of 250,000 theoretical LSPs. For each RBC in blood sample we determined three abovementioned shape characteristics and refractive index, which also allows us to calculate volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration and content. ©2016 Optical Society of America

show abstract

“…A mesh-like spectrin-actin cytoskeleton network is anchored to the phospholipid bilayer and ankyrin proteins (Kim et al, 2012). The underlying spectrin-actin cytoskeletal complex supports the phospholipid bilayer, and it forms a simple hexagonal geometric matrix (Fig.…”

Section: Literature Reviewmentioning

confidence: 99%

Erythrocyte–Platelet Interaction in Uncomplicated Pregnancy

Swanepoel

Pretorius

2014

Microsc Microanal

View full text Add to dashboard Cite

Maternal and fetal requirements during uncomplicated pregnancy are associated with changes in the hematopoietic system. Platelets and erythrocytes [red blood cells (RBCs)], and especially their membranes, are involved in coagulation, and their interactions may provide reasons for the changed hematopoietic system during uncomplicated pregnancy. We review literature regarding RBC and platelet membrane structure and interactions during hypercoagulability and hormonal changes. We then study interactions between RBCs and platelets in uncomplicated pregnancy, as their interactions may be one of the reasons for increased hypercoagulability during uncomplicated pregnancy. Scanning electron microscopy was used to study whole blood smears from 90 pregnant females in different phases of pregnancy. Pregnancy-specific interaction was seen between RBCs and platelets. Typically, one or more platelets interacted through platelet spreading and pseudopodia formation with a single RBC. However, multiple interactions with RBCs were also shown for a single platelet. Specific RBC-platelet interaction seen during uncomplicated pregnancy may be caused by increased estrogen and/or increased fibrinogen concentrations. This interaction may contribute to the hypercoagulable state associated with healthy and uncomplicated pregnancy and may also play a fundamental role in gestational thrombocytopenia.

show abstract

Measurement Techniques for Red Blood Cell Deformability: Recent Advances

Cited by 72 publications

References 135 publications

Stiffness increase of red blood cells during storage

Stiffness increase of red blood cells during storage

Mature red blood cells: from optical model to inverse light-scattering problem

Erythrocyte–Platelet Interaction in Uncomplicated Pregnancy

Contact Info

Product

Resources

About