Measurement of X-ray-induced DNA double-strand breaks at various stages of the cell cycle using the total fluorescence as a comet assay parameter

Attia, Atef; Nabil, Ghada M.; Frankenberg, D.; Frankenberg-Schwager, M.

doi:10.1016/j.radphyschem.2011.06.010

Cited by 4 publications

(3 citation statements)

References 54 publications

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“…These cell types were studied in further experiments. It is known that the sensitivity of cells towards ionizing radiation can be increased by serum deprivation [ 27 ]. Therefore, we monitored in subsequent experiments the impact of different RF doses on induction of DNA damage in U87 and U251 cells cultivated in absence of serum.…”

Section: Resultsmentioning

confidence: 99%

“…In this context, it is notable that earlier experiments with ionizing radiation found that human cells are more sensitive when arrested in G0 phase [ 32 ]. This phenomenon was explained by lower levels of protective sulfhydryl compounds in the cytosol at this stage of the cell cycle [ 27 ]. The induction of DNA damage which was seen under these conditions may be related to the p53 status of the cells as significant increase of DNA damage was only detected in the p53 proficient line U87.…”

Section: Discussionmentioning

confidence: 99%

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Mobile phone specific electromagnetic fields induce transient DNA damage and nucleotide excision repair in serum-deprived human glioblastoma cells

et al. 2018

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Some epidemiological studies indicate that the use of mobile phones causes cancer in humans (in particular glioblastomas). It is known that DNA damage plays a key role in malignant transformation; therefore, we investigated the impact of the UMTS signal which is widely used in mobile telecommunications, on DNA stability in ten different human cell lines (six brain derived cell lines, lymphocytes, fibroblasts, liver and buccal tissue derived cells) under conditions relevant for users (SAR 0.25 to 1.00 W/kg). We found no evidence for induction of damage in single cell gel electrophoresis assays when the cells were cultivated with serum. However, clear positive effects were seen in a p53 proficient glioblastoma line (U87) when the cells were grown under serum free conditions, while no effects were found in p53 deficient glioblastoma cells (U251). Further experiments showed that the damage disappears rapidly in U87 and that exposure induced nucleotide excision repair (NER) and does not cause double strand breaks (DSBs). The observation of NER induction is supported by results of a proteome analysis indicating that several proteins involved in NER are up-regulated after exposure to UMTS; additionally, we found limited evidence for the activation of the γ-interferon pathway. The present findings show that the signal causes transient genetic instability in glioma derived cells and activates cellular defense systems.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mobile phone specific electromagnetic fields induce transient DNA damage and nucleotide excision repair in serum-deprived human glioblastoma cells

et al. 2018

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“…There is a third possibility, introduced later by Chadwick and Leenhouts [5], of a lesion/non-lesion interaction whereby a damaged site, in the Revell sense, may interact with an undamaged chromosome to form an exchange. Several studies have employed X-rays to study their effects on radiation induced genetic damage [6][7][8]. Radiation at doses used in therapy depletes cellular alpha-tocopherol in normal cells, thereby increasing their risk of damage; animal studies show that whole-body exposure to X-ray irradiation decreases tissue concentrations of vitamins C and E [9].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Radioprotective Efficacy of Dendrodoine Analog Against the Formation of Dicentric Aberration Frequency in Cultured Human Peripheral Blood Lymphocytes

Kalpana¹,

Thayalan²,

Menon³

2012

TONUTRAJ

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The present study was aimed to evaluate the protective efficacy of dendrodoine analog (DA), an aminothiazole derivative against the formation of radiation induced dicentric (DC) aberration frequency on cultured human peripheral blood lymphocytes. DA was chemically synthesized and the product thus obtained was purified using column chromatography packed with silica gel using chloroform as the solvent. The purity status of the final product was assessed employing high performance thin layer chromatography (HPTLC). The radioprotective efficacy of DA against the formation of DC aberration frequency was analyzed by pre-incubating human peripheral blood lymphocytes with the optimum concentration of DA, selected from our previous study, followed by exposure to different doses of radiation. The results indicated that there was a dose dependent increase in the formation of DC aberration frequency in the irradiated groups when compared to DA pre-treated groups which modulated the toxic effects of radiation by means of its effective DNA protective and antioxidant property.

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Influence of the dose rate of gamma irradiation and some other conditions on the radiation protection of microbial cells by scavenging of OH radicals

Neužilová

Ondrák

Čuba

et al. 2018

J Radioanal Nucl Chem

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Measurement of X-ray-induced DNA double-strand breaks at various stages of the cell cycle using the total fluorescence as a comet assay parameter

Cited by 4 publications

References 54 publications

Mobile phone specific electromagnetic fields induce transient DNA damage and nucleotide excision repair in serum-deprived human glioblastoma cells

Mobile phone specific electromagnetic fields induce transient DNA damage and nucleotide excision repair in serum-deprived human glioblastoma cells

Evaluating the Radioprotective Efficacy of Dendrodoine Analog Against the Formation of Dicentric Aberration Frequency in Cultured Human Peripheral Blood Lymphocytes

Influence of the dose rate of gamma irradiation and some other conditions on the radiation protection of microbial cells by scavenging of OH radicals

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