Evaluating the Radioprotective Efficacy of Dendrodoine Analog Against the Formation of Dicentric Aberration Frequency in Cultured Human Peripheral Blood Lymphocytes

Abstract

The present study was aimed to evaluate the protective efficacy of dendrodoine analog (DA), an aminothiazole derivative against the formation of radiation induced dicentric (DC) aberration frequency on cultured human peripheral blood lymphocytes. DA was chemically synthesized and the product thus obtained was purified using column chromatography packed with silica gel using chloroform as the solvent. The purity status of the final product was assessed employing high performance thin layer chromatography (HPTLC). The radioprotective efficacy of DA against the formation of DC aberration frequency was analyzed by pre-incubating human peripheral blood lymphocytes with the optimum concentration of DA, selected from our previous study, followed by exposure to different doses of radiation. The results indicated that there was a dose dependent increase in the formation of DC aberration frequency in the irradiated groups when compared to DA pre-treated groups which modulated the toxic effects of radiation by means of its effective DNA protective and antioxidant property.