Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection

Abstract

A method is described for the determination of terbutaline and salbutamol in plasma from patients given maximal therapy for brittle asthma. The analytes were isolated by solid phase extraction on alkali-treated Bond-Elut, unmodified, silica columns and measured by high performance liquid chromatography with fluorescence detection (excitation wavelength 200 nm). The limits of detection for a 1 mL sample containing salbutamol and terbutaline were 1 microgram/L and 2.5 micrograms/L, respectively. The intra-assay precision (CV) for samples containing 25 micrograms/L was 3.6 and 5.0% respectively. This method was applied to the measurement of terbutaline in samples from patients given continuous infusions of the drug to assess whether this treatment might result in toxicity.

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

“…With regards to the detection of salbutamol, several techniques have also been described in the literature, e.g. high performance thin layer chromotography (HPTLC) (Colthup et al 1985), radio-immunoassay (RIA) (Loo et al 1987), high performance liquid chromatography (HPLC) with electrochemical (Oosterhuis and van Boxtel 1982, Tan and Soldin, 1984, Emmet al 1988, Sagar et al 1993 or fluorescence detection (Hutchings et al 1983, Miller and Greenblatt 1986, McCarthy et al 1993, or gas chromatography coupled to mass spectrometry (GC-MS) in the electron impact (El) mode (Martin et al 1976) or under positive chemical ionization (PCI) using ammonia (Leferink etal. 1982, WeisenbergereiaZ.…”

Simple determination of terbutaline in dog plasma by column-switching liquid chromatography

“…The dogs were provided a standardised meal 6 h after drug intake. Blood samples of control group were collected into heparinised polystyrene tubes at time 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 7, 9, 11 and 15 h. Blood samples of test group were collected at time 0, 2, 2.5, 3,4,5,7,8,9,11,12,14,15,18,19,23, 26 and 28 h. Plasma samples were obtained from the supernatant of centrifuged blood and they were immediately frozen and kept at −20 • C until assay. There was a 1-week washing-out time between each treatment period.…”

“…Terbutaline in samples from pharmacokinetic studies has been analysed by GC-MS [2] or capillary electrophoresis [3], however, most investigators have used HPLC. Several HPLC methods have been developed, using MS [4][5][6], electrochemical [7] or fluorescence detection [8] to ensure adequate analytical sensitivity. The determination of drugs in biological fluids using HPLC usually requires several sample preparation processes or off-line solid phase extraction [7], which are time-consuming and may lead to variability in the results.…”

Determination of the enantiomers of salbutamol and its 4-O-sulphate metabolites in biological matrices by chiral liquid chromatography tandem mass spectrometry

“…5,6 There have been numerous methods reported for the determination of racemic b-agonists in biological matrices utilising a variety of analytical techniques and these have been reviewed. 7,8 Both normal phase, 9 but predominantly reversed phase, high performance liquid chromatography (HPLC) methods have been reported for salbutamol using either fluorescence 9,10,11,12 or electrochemical detection. 13,14,15 These HPLC methods have employed either liquid-liquid 11,13 or solid phase extraction (SPE) 9,12,15 procedures and the use of column switching technology has also been reported.…”