Measurement of Sterigmatocystin Concentrations in Urine for Monitoring the Contamination of Cattle Feed

Fushimi, Yasuo; Takagi, Mitsuhiro; Uno, Seiichi; Kokushi, Emiko; Nakamura, Masayuki; Hasunuma, Hiroshi; Shinya, Urara; Deguchi, Eisaburo; Fink‐Gremmels, Johanna

doi:10.3390/toxins6113117

Cited by 14 publications

(19 citation statements)

References 19 publications

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“…Fushimi (Fushimi et al, 2014) investigated STC in urine only. Fushimi (Fushimi et al, 2014) investigated STC in urine only.…”

Section: Enniatins Beauvericin Sterigmatocystinmentioning

confidence: 99%

Mycotoxin mixtures in food and feed: holistic, innovative, flexible risk assessment modelling approach:

Battilani¹,

Palumbo²,

Giorni³

et al. 2020

EFS3

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Mycotoxins are toxic compounds mainly produced by fungi of the genera Aspergillus, Penicillium and Fusarium. They are present, often as mixtures, in many feed and food commodities including cereals, fruits and vegetables. Their ubiquitous presence represents a major challenge to the health and well being of humans and animals. Hundreds of compounds are listed as possible mycotoxins occurring in raw and processed materials destined for human food and animal feed. In this study, mycotoxins of major toxicological relevance to humans and target animal species were investigated in a range of crops of interest (and their derived products). Extensive Literature Searches (ELSs) were undertaken for data collection on: (i) ecology and interaction with host plants of mycotoxin producing fungi, mycotoxin production, recent developments in mitigation actions of mycotoxins in crop chains (maize, small grains, rice, sorghum, grapes, spices and nuts), (ii) analytical methods for native, modified and co-occurring mycotoxins (iii) toxicity, toxicokinetics, toxicodynamics and biomarkers relevant to humans and animals (poultry, suidae (pig, wild boar), bovidae (sheep, goat, cow, buffalo), rodents (rats, mice) and others (horses, dogs), (iv) modelling approaches and key reference values for exposure, hazard and risk modelling. Comprehensive databases were created using EFSA templates and were stored in the MYCHIF platform. A range of approaches were implemented to explore the modelling of external and internal exposure as well as dose-response of mycotoxins in chicken and pigs. In vitro toxicokinetic and in vivo toxicity databases were exploited, both for single compounds and mixtures. However, large data gaps were identified particularly with regards to absence of common statistical and study designs within the literature and constitute an obstacle for the harmonisation of internal exposure and dose-response modelling. Finally, risk characterisation was also performed for humans as well as for two animal species (i.e. pigs and chicken) using available tools for the modelling of internal dose and a component-based approach for selected mycotoxins mixtures.© European Food Safety Authority, 2020 MYCHIF www.efsa.europa.eu/publications 2 EFSA Supporting publication 2020: EN-1757

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“…Fushimi (Fushimi et al, 2014) investigated STC in urine only. Fushimi (Fushimi et al, 2014) investigated STC in urine only.…”

Section: Enniatins Beauvericin Sterigmatocystinmentioning

confidence: 99%

Mycotoxin mixtures in food and feed: holistic, innovative, flexible risk assessment modelling approach:

Battilani¹,

Palumbo²,

Giorni³

et al. 2020

EFS3

View full text Add to dashboard Cite

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“…In vivo studies have revealed an extensive metabolization of STERIG, consequently free STERIG was scarcely detected in the urine of animals (Fushimi et al., ). Fushimi et al.…”

Section: Sterigmatocystinmentioning

confidence: 99%

Mycotoxin Biomarkers of Exposure: A Comprehensive Review

Vidal

Mengelers

Yang³

et al. 2018

Comp Rev Food Sci Food Safe

154

137

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To date, the use of biomarkers has become generally accepted. Biomarker-driven research has been proposed as a successful method to assess the exposure to xenobiotics by using concentrations of the parent compounds and/or metabolites in biological matrices such as urine or blood. However, the identification and validation of biomarkers of exposure remain a challenge. Recent advances in high-resolution mass spectrometry along with new analytical (postacquisition data-mining) techniques will improve the quality and output of the biomarker identification process. Chronic or even acute exposure to mycotoxins remains a daily fact, and therefore it is crucial that the mycotoxins' metabolism is unravelled so more knowledge on biomarkers in humans and animals is acquired. This review aims to provide the scientific community with a comprehensive overview of reported in vitro and in vivo mycotoxin metabolism studies in relation to biomarkers of exposure for deoxynivalenol, nivalenol, fusarenon-X, T-2 toxin, diacetoxyscirpenol, ochratoxin A, citrinin, fumonisins, zearalenone, aflatoxins, and sterigmatocystin.

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“…All urine samples were analysed using LC‐MS/MS according to our previous study (Fushimi et al., ; Takagi et al., ). Briefly, 0.5 ml of a urine sample was mixed with 3.0 ml of 50 m m ammonium acetate buffer (pH 4.8) and 10 μl of glucuronidase/arylsulfatase solution and incubated for 12 hr at 38°C.…”

Section: Methodsmentioning

confidence: 99%

“…Creatinine concentrations in the urine were determined using a commercial kit, the Sikarikit‐S CRE (Kanto Chemical, Tokyo, Japan) following the manufacturer's instructions, and measured using the BM8060 Clinical Analyzer (Nihon‐Denshi, Tokyo, Japan). The urinary concentrations of ZEN, α‐ZOL, β‐ZOL and STC were expressed as a ratio to creatinine (pg/mg Crea), as described previously (Fushimi et al., ; Takagi et al., ).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we established a novel monitoring system for ZEN and its metabolites, and STC exposure using urinary analyses in dairy herds (Fushimi et al., ; Takagi et al., ). Considering the likely exposure of horses to mycotoxin‐contaminated feed materials or bedding (straw) (Séguin et al., ; Wichert et al., ), we aimed at establishing a similar monitoring system for horses.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of urinary concentrations of the mycotoxins zearalenone and sterigmatocystin as biomarkers of exposure in mares

Takagi

Uno

Kokushi

et al. 2017

Reprod Domestic Animals

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Mycotoxins may affect animal health, including reproduction. Little is known about the clinical relevance of exposure of horses to contaminated feed. This study aimed at (i) monitoring the levels of the mycotoxins zearalenone (ZEN), with its metabolites α- and β-zearalenol (α- and β-ZOL), and sterigmatocystin (STC) in urine samples from thoroughbred mares in Japan and (ii) relating these findings to the potential effects on reproductive efficacy of breeding mares. Sixty-three urine samples of breeding mares from 59 breeding farms were used. Urine samples and reproductive records were collected from each mare when it was presented to the stallion station. Urinary concentrations of ZEN, α- and β-ZOL, and STC were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). ZEN, α- and β-ZOL were measurable in the urine of all examined mares, indicating the prevalence of ZEN in equine feeds. In seven of the 63 samples, STC was also detected at levels ranging from 1.3 to 18.0 pg/mg creatinine. No significant correlation between the concentrations of mycotoxins and pregnancy status was observed. In conclusion, measurement of mycotoxins in urine samples is a useful non-invasive method for monitoring the systemic exposure of mares to multiple mycotoxins.

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Measurement of Sterigmatocystin Concentrations in Urine for Monitoring the Contamination of Cattle Feed

Cited by 14 publications

References 19 publications

Mycotoxin mixtures in food and feed: holistic, innovative, flexible risk assessment modelling approach:

Mycotoxin mixtures in food and feed: holistic, innovative, flexible risk assessment modelling approach:

Mycotoxin Biomarkers of Exposure: A Comprehensive Review

Measurement of urinary concentrations of the mycotoxins zearalenone and sterigmatocystin as biomarkers of exposure in mares

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