Measurement of specific IgE antibodies to Ses i 1 improves the diagnosis of sesame allergy

Abstract

Sensitization to Ses i 1 is strongly associated with clinical sesame allergy. Measurement of specific IgE to rSes i 1 could reduce the numbers of OFCs needed.

Evaluation of the basophil activation test and skin prick testing for the diagnosis of sesame food allergy

“…Two of these studies reported critical values generating >95% specificity for commercial SPT, yet with sensitivities reaching <50%. In terms of specific serum IgE, >95% specificity was obtained with sensitivities <30%, while the study investigating serum reactivity to rSesi1 yielded specificity and sensitivity of 85% . SPT remains a critical diagnostic tool, in that it is a relatively straightforward inexpensive technique, with a quick readout.…”

“…Of the patients whose BAT results for both CD63 and CD203c were below the cut-offs, 12 were not allergic and one patient was allergic. Two patients, one with SFA and one without, Regarding other methods for diagnosis of SFA, SPT, 7,9,10,17 detection of specific serum IgE against sesame protein [8][9][10]17,26 and recently, specific component testing against a recombinant Sesi 1 (rSes i1) allergen 27 have been reported in the literature. Two of these studies 7,10 reported critical values generating >95% specificity for commercial SPT, yet with sensitivities reaching <50%.…”

“…In terms of specific serum IgE, >95% specificity was obtained with sensitivities <30%, [8][9][10] while the study investigating serum reactivity to rSesi1 yielded specificity and sensitivity of 85%. 27 SPT remains a critical diagnostic tool, in that it is a relatively straightforward inexpensive technique, with a quick readout. Our analysis therefore began with SPT, testing 2 formulations, and finding that a high protein extract which could be reproducibly generated was more accurate than commercial extract in detecting SFA.…”

Component‐resolved diagnostics demonstrates that most peanut‐allergic individuals could potentially introduce tree nuts to their diet

“…Almond and hazelnut are strongly birch cross‐reactive tree nuts, which can be seen in our results of the prevalence of skin prick test positivity and the asymptomatic sensitization findings in the patients that have tried these species despite of their skin prick test positivity. We consider the available species‐specific tests, when negative, sufficient to rule out hazelnut, cashew and pistachio, walnut, Brazil nut, and sesame seed allergies in most cases. Species‐specific pecan sensitizations were assessed by analysing the cross‐reactive walnut allergens .…”

Sesame allergy: current perspectives

“…However, to date very few studies have evaluated this technique in sesame allergy. In one study of 92 children sensitized to sesame, sensitization to a recombinant form of the protein 2S albumin (rSes i 1) had a comparable sensitivity to the commercial assay measuring sIgE (86.1% for rSes i 1 compared to 83.3% for the commercial assay) but significantly better specificity (85.7% compared to 48.2%) for sesame allergy 22. Teodorowicz et al23 identified the major allergens in six sesame-allergic patients and found that oleosin was the dominant allergen in the five patients who had a history of anaphylaxis to sesame, leading to the hypothesis that sensitization to oleosin may identify a more severe sesame-allergic phenotype.…”