Measurement of Small Quantities of Insulin-Like Activity With Rat Adipose Tissue. Iv. Serum Insulin-Like Activity and Tumor Insulin Content in Patients With Functioning Islet-Cell Tumors*

Steinke, Jürgen; Soeldner, J S; Renold, Albert E.

doi:10.1172/jci104816

Cited by 99 publications

(15 citation statements)

References 20 publications

(15 reference statements)

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“…Our patient's response to secretogogues was similar to that recently reported for another atypical insulinoma [25]. The extractable insulin of the tumor (20 U per gram wet weight) and the proportion of proinsulin (23%) are considerably higher than those of normal human pancreas [26,27]. An insulinoma consisting of a homogeneous population of islet-like cells might be compared more appropriately to isolated islets than to whole pancreas, which contains only about one percent islet tissue.…”

Section: Discussionsupporting

confidence: 82%

Human islet cell adenoma: Metabolic analysis of the patient and of tumor cells in monolayer culture

et al. 1975

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Summary. Cell cultures were established from a benign pancreatic islet adenoma. Over 200 ~tU/culture/day immunoreactive insulin were found in culture media. Cultures with medium 199 released insulin for about 2 months; those with medium F12K were maintained for over 7 months, and have been successfully subcultured. Increasing culture medium glucose to 326 mg per 100 ml, alone or with lencine (10 raM) or theophylline (2 raM), failed to increase insulin release above baseline. Studies in the patient prior to surgery using oral glucose, leucine, beef meal, intravenous tolbutamide, and glueagon failed to increase plasma insulin and thus were consistent with cell culture responses. Extracts of tumor tissue contained 23 % proinsulin-like material; high insulin containing samples of culture medium had 5% proinsulin and less than 40 pg glucagon/ml. Aldehyde fuchsin positive granulation was sparse in both cultured cells and the original tumor. These studies demonstrate long term viability, in monolayer culture, of cells derived from this islet cell adenoma, with retention of secretory characteristics consistent with data obtained prior to removal of the adenoma from the patient.

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Section: Discussionsupporting

confidence: 82%

Human islet cell adenoma: Metabolic analysis of the patient and of tumor cells in monolayer culture

et al. 1975

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“…Franckson et al (1962) and Steinke, Soeldner & Renold (1963) used the rat adipose tissue assay to estimate the levels of serum insulin-like activity in normal human subjects during an intra¬ venous glucose tolerance test (0-33-0-5 g./kg.). Franckson et al (1962) and Steinke, Soeldner & Renold (1963) used the rat adipose tissue assay to estimate the levels of serum insulin-like activity in normal human subjects during an intra¬ venous glucose tolerance test (0-33-0-5 g./kg.).…”

Section: Discussionmentioning

confidence: 99%

The Insulin Activity of Bovine and Ovine Blood Plasma

Nf¹

1964

Journal of Endocrinology

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The insulin-like activities of ovine blood plasma diluted fourfold, and of acid-ethanol extracts of plasma taken before, and at various times up to 2 hr. after the intravenous injection of glucose (0\ m=. \ 1\ p=n-\ 1\ m=. \ 1g./kg.), were determined by the rat diaphragm assay.Diluted plasma generally showed a transient increase in insulin-like activity after the injection of glucose, but the magnitude of the increase was not related to the amount of glucose injected. Starvation for 3 days did not abolish the effect of glucose on the insulin-like activity of diluted plasma in two out of four sheep, although all four animals showed a reduced tolerance to glucose.Increases in the insulin-like activity of extracts of plasma were observed in two experiments only, when 1\m=.\1 g. glucose/kg. had been administered. The results in the other experiments were variable, and the possible significance of these variations is discussed.The results on sheep are compared with those obtained in monogastric animals by other workers. introduction In man intravenous or oral administration of glucose is followed by a rise in the insulin-like activity of systemic blood plasma. This has been demonstrated by the rat diaphragm technique (

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“…Serum insulin-like activity (ILA) was estimated on serum diluted 1 to 4 with buffer (25% serum) with the rat epididymal adipose tissue technique previously described (18). In some instances, ILA was extracted from serum by an acid ethanol procedure, previously described (19 Heifers injected wuith porcine insulin in adjuvant. Adjuvant that contained porcine insulin was administered usually every other week in all five animals of this group from May to August 1962, for a total of seven injections.…”

Section: Methodsmentioning

confidence: 99%

Immunological response to the prolonged administration of heterologous and homologous insulin in cattle.

Renold¹,

Steinke²,

Soeldner³

et al. 1966

J. Clin. Invest.

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Measurement of Small Quantities of Insulin-Like Activity With Rat Adipose Tissue. Iv. Serum Insulin-Like Activity and Tumor Insulin Content in Patients With Functioning Islet-Cell Tumors*

Cited by 99 publications

References 20 publications

Human islet cell adenoma: Metabolic analysis of the patient and of tumor cells in monolayer culture

Human islet cell adenoma: Metabolic analysis of the patient and of tumor cells in monolayer culture

The Insulin Activity of Bovine and Ovine Blood Plasma

Immunological response to the prolonged administration of heterologous and homologous insulin in cattle.

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