1977
DOI: 10.1136/jcp.30.7.645
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Measurement of serum folate: experience with 75Se-selenofolate radioassay.

Abstract: SUMMARY Three hundred and eighty-two serum samples were assayed for folate content by a standard Lactobacillus casei microbiological assay and by radioassay using the 75Se-selenofolate radioassay kit from the Radiochemical Centre, Amersham. Results showed encouraging concordance. Explanations were discovered for the few disparities. The radioassay procedure is simple and an assay batch can be completed in one working day.We wish to document our results from measurements of serum folate levels by both microbiol… Show more

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Cited by 15 publications
(10 citation statements)
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“…The myriad forms of folates have hindered development of analytic techniques that detect specific congeners and the majority of measurements are reported as total folate. Popular methods for folate measurement include microbiological growth assays that use folate-responsive microorganisms (8 -11) or radiolabeled folate binder assays that operate on the principle of competitive binding between endogenous and exogenous (radiolabeled folate) folates for a limited amount of a protein binder (12)(13)(14)(15)(16)(17)(18). Folate assays that involve the use of HPLC (19 -22) and GCMS (23) methods for blood and tissues are less commonly used, but have the potential for providing superior specificity and analytic precision.…”
mentioning
confidence: 99%
“…The myriad forms of folates have hindered development of analytic techniques that detect specific congeners and the majority of measurements are reported as total folate. Popular methods for folate measurement include microbiological growth assays that use folate-responsive microorganisms (8 -11) or radiolabeled folate binder assays that operate on the principle of competitive binding between endogenous and exogenous (radiolabeled folate) folates for a limited amount of a protein binder (12)(13)(14)(15)(16)(17)(18). Folate assays that involve the use of HPLC (19 -22) and GCMS (23) methods for blood and tissues are less commonly used, but have the potential for providing superior specificity and analytic precision.…”
mentioning
confidence: 99%
“…Details of the method recommended for serum folate assay were described by Johnson et al (1977). Received …”
Section: Folate Radioassaymentioning
confidence: 99%
“…These were placed in a water bath at 100°C for five minutes and then cooled to ambient temperature. The method then followed the protocol described (Johnson et al, 1977).…”
Section: Effects Of Heatingmentioning
confidence: 99%
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“…The radioassay kit (The Radiochemical Centre, Amersham, Bucks, UK) utilised 75Se labelled folate (Johnson et al, 1977). Supernatants were counted on a Wallac 300 gamma counter (LKB, South Croydon, Surrey, UK) and the results were calculated as recommended (Radiochemical Centre, 1976).…”
mentioning
confidence: 99%