2000
DOI: 10.1006/abio.2000.4660
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Determination of Blood Folate Using Acid Extraction and Internally Standardized Gas Chromatography–Mass Spectrometry Detection

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Cited by 34 publications
(34 citation statements)
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References 33 publications
(25 reference statements)
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“…On average, 1-2 g of total RNA was isolated from five 5-m tissue sections. For each sample, 200 ng of total RNA was converted to cDNA and processed in the DASL assay as described previously (11,13 ). Oligonucleotides targeting 502 cancer-related genes were used in these experiments, at a density of three nonoverlapping probes per gene, giving a 1506-plex measurement for each sample.…”
Section: Distribution Of Results For Metanephrinementioning
confidence: 99%
See 1 more Smart Citation
“…On average, 1-2 g of total RNA was isolated from five 5-m tissue sections. For each sample, 200 ng of total RNA was converted to cDNA and processed in the DASL assay as described previously (11,13 ). Oligonucleotides targeting 502 cancer-related genes were used in these experiments, at a density of three nonoverlapping probes per gene, giving a 1506-plex measurement for each sample.…”
Section: Distribution Of Results For Metanephrinementioning
confidence: 99%
“…Oligonucleotides targeting 502 cancer-related genes were used in these experiments, at a density of three nonoverlapping probes per gene, giving a 1506-plex measurement for each sample. Our previous study showed that three probes per gene lend the assay sufficient sensitivity and reproducibility for quantitative detection of differential expression in FFPE tissues (13 ). Mean signal values were computed for each gene by determining the mean signal for the three representative probes (11 ).…”
Section: Distribution Of Results For Metanephrinementioning
confidence: 99%
“…Between-day imprecision was measured by assaying the same samples on 20 different days over a 3-week period. (27 ). Fig.…”
Section: Recovery and Imprecisionmentioning
confidence: 99%
“…Gas chromatography/mass spectrometry used to analyze WBF after cleavage of folates to p-aminobenzoic acid showed greater sensitivity than previous chromatographic methods and used for the first time an isotopelabeled internal standard (IS). The method, however, requires a complex and lengthy multistep sample preparation including chemical derivatization (11,(13)(14)(15). We describe here the first automated 96-well plate stableisotope-dilution liquid chromatography-tandem mass spectrometry (LC/MS/MS) method that measures intact folate monoglutamates in conventionally prepared whole blood (WB) lysates and serum.…”
mentioning
confidence: 99%