Measurement of serum ferritin by a 2-site immunoradiometric assay

Miles, Laughton E.; Lipschitz, David A.; Bieber, Charles P.; Cook, James D.

doi:10.1016/0003-2697(74)90347-9

Cited by 454 publications

(163 citation statements)

References 17 publications

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“…Transferrin saturation (TS) was calculated from serum iron and serum transferrin concentrations (Lockitch, 1993). Serum ferritin (SF) was analysed in duplicate by two-site radio-immunoassay based on the method of Miles et al (1974) using ferritin kits (Quantimune Ferritin IRMA, Biorad Laboratories, Mississauga, Ont.). Lyphocheck anaemia control serum and three levels of Lyphocheck control sera (levels 1, 2 and 3) were used to assess accuracy and precision.…”

Section: Methodsmentioning

confidence: 99%

The impact of malarial infection and diet on the anaemia status of rural pregnant Malawian women

1999

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Objective: To investigate haematological and biochemical iron indices in relation to malaria, gravida, and dietary iron status in rural pregnant Malawian women. Design: In this self-selected sample, haemoglobin, haematocrit, red cell indices, serum ferritin, serum iron, serum transferrin, and serum transferrin receptor (TfR) were measured. Infection was assessed by a malaria slide, serum C-reactive protein, and white blood cell count. Dietary iron variables were measured by three 24-h interactive recalls. Setting and subjects: 152 rural pregnant women recruited at 24 weeks gestation while attending a rural antenatal clinic in Southern Malawi; 36% were primagravid; 43% were gravida 2 ± 4; 26% were gravida b 5. Results: Of the women, 69% (n 105) were anaemic (haemoglobin`110 gal); 37% (n 39) had anaemia and malarial parasitaemia on the test day; 17% (n 26) with malaria were also classi®ed with iron de®ciency (ID) anaemia (based on serum ferritin 50 mgal and Hb`110 gal) while an additional seven with malaria were classi®ed with ID without anaemia. In malarial-free subjects, 32% were classi®ed with IDA (serum ferritiǹ 12 mgal and Hb`110 gal) and 17% with ID (serum ferritin`12 mgal; Hb ! 110 gal). Serum TfR concentrations were elevated in anaemic women (P`0.01). In non-malarial parasitaemic subjects, serum TfR correlated negatively with haemoglobin (r 7 0.313; P`0.001) but not serum ferritin. Of the women, 49% were at risk for inadequate iron intakes. Most dietary iron was non-haem; plant foods provided 89%;¯esh foods (mainly ®sh) only 9%. Malarial parasitaemia and intakes of available iron impacted signi®cantly on iron status. Conclusion: Anaemia prevalence from all causes was high (that is, 69%); three factors were implicated: malaria, and de®ciencies of iron and possibly folate, induced partly by an inadequate dietary supply andaor secondary to malarial parasitaemia.

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Section: Methodsmentioning

confidence: 99%

The impact of malarial infection and diet on the anaemia status of rural pregnant Malawian women

1999

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“…For the measurement of serum proteins, serum was separated by centrifugation within 1 h of collection and stored at −20°C for up to 2 months, then stored at −80°C until shipment for analyses. Serum ferritin, C-reactive protein (CRP) and soluble transferrin receptor were measured using standard laboratory techniques in a central laboratory (VitA Iron Lab, Wilstaett Germany) (18)(19)(20) .…”

Section: Anaemia and Fe Statusmentioning

confidence: 99%

“…At 18 months of age, infants in the cereal group had higher Hb concentration than infants in the control group (10·7 v. 10·1 g/dl, P = 0·03) and fewer were anaemic (26 % v. 50 %, Table 2 Anthropometric data at baseline (6 months), during (9 and 12 months) and at the end (18 …”

Section: Anaemia and Fe Statusmentioning

confidence: 99%

A cluster-randomized trial determining the efficacy of caterpillar cereal as a locally available and sustainable complementary food to prevent stunting and anaemia

Bauserman

Lokangaka

Gado

et al. 2015

Public Health Nutr.

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Objective: We conducted a cluster-randomized controlled trial to assess the efficacy of a cereal made from caterpillars, a micronutrient-rich, locally available alternative animal-source food, on reducing stunting and anaemia in infants in the Democratic Republic of Congo. Design: Six-month-old infants were cluster randomized to receive either caterpillar cereal daily until 18 months of age or the usual diet. At 18 months of age, anthropometric measurements and biological samples were collected. Setting: The rural Equateur Province in the Democratic Republic of Congo. Subjects: One hundred and seventy-five infants followed from 6 to 18 months of age. Results: Stunting was common at 6 months (35 %) and the prevalence increased until 18 months (69 %). There was no difference in stunting prevalence at 18 months between the intervention and control groups (67 % v. 71 %, P = 0·69). Infants in the cereal group had higher Hb concentration than infants in the control group (10·7 v. 10·1 g/dl, P = 0·03) and fewer were anaemic (26 v. 50 %, P = 0·006), although there was no difference in estimates of body Fe stores (6·7 v. 7·2 mg/kg body weight, P = 0·44). Conclusions: Supplementation of complementary foods with caterpillar cereal did not reduce the prevalence of stunting at 18 months of age. However, infants who consumed caterpillar cereal had higher Hb concentration and fewer were anaemic, suggesting that caterpillar cereal might have some beneficial effect. The high prevalence of stunting at 6 months and the lack of response to this micronutrient-rich supplement suggest that factors other than dietary deficiencies also contribute to stunting.

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“…Serum ferritin is directly proportional to body iron stores and concentration <12 ng/ml reflects an iron-depleted state. [9][10][11][12][13] The aim of this study was to evaluate the frequency of iron deficiency in Santa Casa Hemocenter blood donors and to establish the frequency of iron deficiency in blood donors according to sex, whether they were first-time or multi-time donors, and the blood donation frequency per year.…”

Section: Introductionmentioning

confidence: 99%

Iron deficiency in blood donors

Cançado¹,

Chiattone²,

Alonso³

et al. 2001

Sao Paulo Med. J.

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CONTEXT: Blood donation results in a substantial loss of iron (200 to 250 mg) at each bleeding procedure (425 to 475 ml) and subsequent mobilization of iron from body stores. Recent reports have shown that body iron reserves generally are small and iron depletion is more frequent in blood donors than in non-donors. OBJECTIVE: The aim of this study was to evaluate the frequency of iron deficiency in blood donors and to establish the frequency of iron deficiency in blood donors according to sex, whether they were first-time or multi-time donors, and the frequency of donations per year. DESIGN: From September 20 to October 5, 1999, three hundred blood donors from Santa Casa Hemocenter of São Paulo were studied. DIAGNOSTIC TESTS: Using a combination of biochemical measurements of iron status: serum iron, total iron-binding capacity, transferrin saturation index, serum ferritin and the erythrocyte indices. RESULTS: The frequency of iron deficiency in blood donors was 11.0%, of whom 5.5% (13/237) were male and 31.7% (20/63) female donors. The frequency of iron deficiency was higher in multi-time blood donors than in first-time blood donors, for male blood donors (7.6% versus 0.0%, P < 0.05) and female ones (41.5% versus 18.5%, P < 0.05). The frequency of iron deficiency found was higher among the male blood donors with three or more donations per year (P < 0.05) and among the female blood donors with two or more donations per year (P < 0.05). CONCLUSIONS: We conclude that blood donation is a very important factor for iron deficiency in blood donors, particularly in multi-time donors and especially in female donors. The high frequency of blood donors with iron deficiency found in this study suggests a need for a more accurate laboratory trial, as hemoglobin or hematocrit measurement alone is not sufficient for detecting and excluding blood donors with iron deficiency without anemia.

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Measurement of serum ferritin by a 2-site immunoradiometric assay

Cited by 454 publications

References 17 publications

The impact of malarial infection and diet on the anaemia status of rural pregnant Malawian women

The impact of malarial infection and diet on the anaemia status of rural pregnant Malawian women

A cluster-randomized trial determining the efficacy of caterpillar cereal as a locally available and sustainable complementary food to prevent stunting and anaemia

Iron deficiency in blood donors

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