Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices

Bridging the academic–industrial gap: application of an oxygen and pH sensor-integrated lab-on-a-chip in nanotoxicology

“…was combined with 54.8 mg of microcrystalline silanized Egyptian Blue as reference. 21 Both luminophores were suspended in 1380 mg of a solution of hydrogel Hydromed D4 (8 % w/w) in THF/water (9 + 1), leading to an indicator dye concentration of 0.2 % w/w and a reference compound concentration of 33.3 % w/w after evaporation of the solvents. Homogenization of the sensor formulations was accomplished utilizing a sonifier (Branson) with ten pulses (1 s) and nine seconds cooldown intervals.…”

“…To calibrate the pH sensor spots, the signal of each spot was recorded after exposing each chamber with buffers of 8 different pH values ranging from pH 4-10. The cotangents of the measured signal were plotted against the pH values, and the calibration function was obtained by fitting with a Boltzmann sigmoidal equation (as previously described 21 ) in a data analysis program (GraphPad Prism). Following cell seeding (5.5x10 3 cells/chamber) and an attachment period of three hours, medium perfusion (5 µL/min) commenced for at least 16 hours (e.g., overnight proliferation) before nanoparticle exposure.…”

“…20 We have recently demonstrated that luminescent oxygen and pH sensor spots can readily be integrated into microfluidic devices and are capable of monitoring cellular responses to different drug exposure scenarios in a dynamic microenvironment. 21 As such, optical sensing strategies provide the optimal basis for the assessment of crucial cellular parameters such as metabolic activity in the context of industrially relevant nanotoxicological screening platforms.…”

Microfluidic-Based Oxygen (O2) Sensors for On-Chip Monitoring of Cell, Tissue and Organ Metabolism

“…Therefore, they have high O 2 sensing sensitivity [39,44,55,56]. Few examples of the most used dyes are Platinum(II) tetrakis(pentafluorophenyl)porphyrin (PtTFPP) [57], Platinium (II) octaethylporphyrin (PtOEP) [42], Platinum (II)-meso-tetra(4fluorophenyl)tetrabenzoporphyrin (PtTPTBPF) [43], Ruthenium-tris(4,7-diphenyl-1,10phenanthroline) dichloride (Ru(dpp)) [55], and Singlet O 2 sensor green (SOSG) [56]. In a few studies, other materials such as polymerized 2-hydroxyethyl methacrylate (HEMA) have been used as a dye [58].…”

Peristaltic on-chip pump for tunable media circulation and whole blood perfusion in PDMS-free organ-on-chip and Organ-Disc systems

“…Reference source not found.). Future disc generations, could integrate oxygen sensors 52 to allow for simultaneous monitoring of oxygen levels and cell metabolism.…”