Measurement of regional cerebral blood flow with antipyrine-14C in awake cats.

Reivich, Martin; Jehle, Jane; Sokoloff, Louis; Kety, Seymour S.

doi:10.1152/jappl.1969.27.2.296

Cited by 479 publications

(132 citation statements)

References 2 publications

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“…The brain was sectioned in a cryostat ( -20°C), and the 20-lLm sections were rapidly dried on a hot plate (60°C) and subsequently exposed, along with six [I4CJmethyi methacrylate calibrated standards, to a single-emulsion X-ray film (Kodak SB-5) for 11 days. Following film de velopment, optical densities were determined with an auto-scanning densitometer (Optronics, P-lOOO, Interna tional, Inc.) with an aperture of 200 ILm, and all data were collected on-line with a Prime computer for calcu lation of LCBF according to the equation of Reivich et al (1969), as modified by Sakurada et al (1978). Differences between groups, with respect to physio logic parameters, were evaluated by one-way analysis of variance (ANOVA, F ratio), followed by the Newman Keuls test where appropriate (Zar, 1974).…”

Section: Lcbf Quantitative Autoradiographymentioning

confidence: 99%

Local Cerebral Blood Flow with Fentanyl-Induced Seizures

Maekawa¹,

Tommasino²,

Shapiro³

1984

J Cereb Blood Flow Metab

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Summary: Local cerebral blood flow (LCBF) was eval uated with the [14C]iodoantipyrine quantitative autoradio graphic technique in 29 brain structures in conscious con trol rats and during fentanyl-induced electroencephalo graphic (EEG) spike and/or seizure activity and in the postseizure EEG suppression phase. During spike ac tivity, LCBF increased in all structures; the increase reached statistical significance (p < 0.05) in the superior colliculus, sensorimotor cortex, and pineal body (+ 130%, + 187%, and + 185% from control, respec tively). With progressive development of seizure activity, LCBF significantly increased in 24 brain structures (range, +58% to +231% from control). During the post seizure EEG suppression phase, LCBF remained ele vated in all structures (+ 80% to + 390% from control).High-dose narcotic anesthesia can elicit seizure activity in laboratory animals as well as under clin ical conditions (De Castro et aI., 1979; Sebel et aI., 1981; Bovill et aI., 1982; Rao et aI., 1982). In a re cent report, the increase in brain oxygen uptake due to fentanyl-elicited convulsive activity was not matched by an equivalent compensatory elevation in overall CBF (Carlsson et aI., 1982). This finding suggests that cerebral ischemia could result due to This paper was presented in part at the Annual Meeting of the American Society of Anesthesiologists, Las Vegas, Nevada, Oc tober 1982.Dr. Maekawa is a Research Fellow in Neuroanesthesia Re search at the University of California at San Diego; he is also affiliated with the Department of Anesthesiology, Yamaguchi University, Yamaguchi, Japan.Dr. Tommasino is a Research Fellow in Neuroanesthesia Re search at the University of California at San Diego; she is also affiliated with the Department of Anesthesia, University of Na ples, First Medical School, Italy.Address correspondence and reprint requests to Dr. Shapiro at Neuroanesthesia Research, M-004, University of California at San Diego, La Jolla, California 92093, U.S.A.Abbreviations used: CMR, Cerebral metabolic rate; LCBF, local cerebral blood flow; LCMR g 1u, local cerebral metabolic rate for glucose; LCVR, local cerebrovascular resistance. 88The local cerebrovascular resistance (LCYR) signifi cantly decreased in 10 of 29 structures with the onset of spike activity (range, -24% to -64%), and remained decreased in all brain structures during seizure activity (range, -34% to -67%) and during the EEG suppression phase (range, -24% to -74%). This reduction of LCVR METHODS Local cerebral blood flow (LCBF) was measured in 23Sprague-Dawley rats with the [14C]iodoantipyrine quan titative autoradiographic technique (Reivich et aI., 1969; Sakurada et a1., 1978). The rats weighed 289 ± 20 g (mean ± SD) and were studied while conscious and during fen tanyl administration, resulting in electroencephalographic (EEG) patterns consisting of spike, seizure, and postsei zure suppression activity. Fentanyl-treated animalsThe rats were briefly anesthetized with 1.5% halothane and 70% nitrous oxide in oxygen to permit tracheost...

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Section: Lcbf Quantitative Autoradiographymentioning

confidence: 99%

Local Cerebral Blood Flow with Fentanyl-Induced Seizures

Maekawa¹,

Tommasino²,

Shapiro³

1984

J Cereb Blood Flow Metab

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“…The autoradiographic techniques developed by Reivich et al (1969), Sakurada et al (1978). and Sokoloff et al (1977) allow evaluation of local CBF (LCBF) or local CMRg1u (LCMRg1u) in animals.…”

mentioning

confidence: 99%

Subarachnoid Hemorrhage in the Rat: Cerebral Blood Flow and Glucose Metabolism during the Late Phase of Cerebral Vasospasm

Delgado

Arbab

Diemer

et al. 1986

J Cereb Blood Flow Metab

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Summary: A double-isotope technique for the simulta neous measurement of CBF and CMRglu was applied to a subarachnoid hemorrhage (SAH) model in the rat. Cis ternal injection of 0.07 ml blood caused a rather uniform 20% reduction in CBF together with an increase in glu cose utilization of 30% during the late phase of vaso spasm. In one-third of the SAH animals, there were focal areas where the flow was lowered to 30% of the control values and the glucose uptake increased to �250% of control. We suggest that blood in the subarachnoid space via a neural mechanism induces the global flow and meta bolic changes, and that the foci are caused by vasospasm superimposed on the global flow and metabolic changes. In the double-isotope autoradiographic technique,

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“…The scaled curves were used to model cerebral blood flow by substitution into the operational equation (Reivich et al, 1969). Calculated flow was found to be lower when measured using the 99mTc chest curve.…”

Section: Discussionmentioning

confidence: 99%

“…A short catheter was in serted into the common carotid artery. The smearing characteristics of the cannula were evaluated using the methods described by Reivich et al (1969) in their orig inal experiments. The isotopes were infused into a fem oral vein at a variable rate, such that the arterial concen tration increased linearly over 1 min.…”

Section: Methodsmentioning

confidence: 99%