A Modification of the Method for the Measurement of Cerebral Blood Flow Using [<sup>14</sup>C]Iodoantipyrine in Small Animals

Richards, Hugh K.; Lovick, A. H. J.; Pickard, John D.

doi:10.1038/jcbfm.1987.18

Cited by 16 publications

(9 citation statements)

References 4 publications

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“…The method has been modified to overcome the need for continuous arte rial sampling in small animals by the simultaneous admin istration of [99mTc]pertechnate (Richards et al, 1987). Both [14C]iodoantipyrine at 5 MBq/kg body weight (Am ersham International) and [99mTc]pertechnate (10 MBq) in 0.4 ml saline were infused over 1 min at an increasing rate.…”

Section: Lcbf Measurementmentioning

confidence: 99%

Local Cerebral Blood Flow in Rats with Congenital Hydrocephalus

Jones

Richards

Bucknall

et al. 1993

J Cereb Blood Flow Metab

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Local cerebral blood flow (LCBF) has been measured by [14C]iodoantipyrine quantitative autoradiography in H-Tx rats with inherited congenital hydrocephalus at 10, 21, and 30 days after birth. LCBF at 10 days was uniformly low in all ten brain regions studied and not significantly different between hydrocephalic and age-matched control rats. By 21 days LCBF had increased significantly in control rats. LCBF was significantly lower (<69%) in the hydrocephalics in all cortical regions and in the inferior colliculus LCBF at both 21 and 30 days. The cerebellar cortex, pons, and caudate were not significantly affected. At 30 days LCBF ranged from 55 to 115 ml 100 g−1 min−1 for hydrocephalics and from 100 to 183 ml 100 g−1 min−1 for controls.

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Section: Lcbf Measurementmentioning

confidence: 99%

Local Cerebral Blood Flow in Rats with Congenital Hydrocephalus

Jones

Richards

Bucknall

et al. 1993

J Cereb Blood Flow Metab

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“…, 1986) or cats (Reivich et aI., 1968(Reivich et aI., , 1969 and newborn dogs (Batton et aI., 1983;Cavazutti and Duffy, 1982;Kennedy et aI., 1972) or monkeys (Hosokawa et aI., 1977). Recently, Richards et al (1987) proposed a modification of the e4C]iodoantipyrine technique for small animals by using a simultaneous infusion of e4C]iodoantipyrine and of [99mTc]pertechnetate. The 99mTc is counted externally by a detector placed over the chest.…”

mentioning

confidence: 99%

Postnatal Changes in Local Cerebral Blood Flow Measured by the Quantitative Autoradiographic [¹⁴C]Iodoantipyrine Technique in Freely Moving Rats

Nehlig

Vasconcelos

Boyet

1989

J Cereb Blood Flow Metab

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Summary:The postnatal changes in local cerebral blood flow in freely moving rats were measured by means of the quantitative autoradiographic e 4C]iodoantipyrine method. The animals were studied at 10, 14, 17, 21, and 35 days and at the adult stage. At 10 days after birth, rates of blood flow were very low and quite homogeneous in most cerebral structures except in a few posterior areas. From these relatively uniform levels, values of local ce rebral blood flow rose notably to reach a peak at 17 days in all brain regions studied. Rates of blood flow decreased between 17 and 21 days after birth and then increased from weaning time to reach the known characteristic dis tribution of the adult rat. The postnatal evolution of local cerebral blood flow in the rat is in good agreement with Local cerebral blood flow (LCBF) techniques have been extensively used to map changes in ce rebral metabolic rate in a variety of animal species including rat, cat, piglet, sheep, dog, monkey, and humans (Ca vazutti and Duffy, 1982; Flecknell et aI., 1983; Hascoet et aI. , 1988; Hosokawa et aI. , 1977; Kennedy et aI., 1972;Lassen and Ingvar, 1972; Plotkine et aI., 1986; Raichle et aI. , 1983; Reivich et aI., 1968 Reivich et aI., , 1969 Rosenberg et aI. , 1982; Sakurada et aI. , 1978;Sokoloff, 1961). Indeed, it has been shown in adult animals that in most phys iological or pharmacological states, functional ac tivity in the different structures of the central ner vous system regulates the local rate of energy me tabolism and that LCBF is coupled to local metabolic demand (Des Rosiers et aI., 1974; McCulReceived December 12, 1988; revised March 30, 1989; ac cepted April 7, 1989 579previous studies in other species such as dog and humans that also show higher rates of cerebral blood flow and glucose utilization at immature stages. However, in the rat, local cerebral blood flow and local cerebral glucose utilization are not coupled over the whole postnatal pe riod studied, since blood flow rates reach peak values at 17 days whereas glucose utilization remains still quite low at that stage. The high rate of cerebral blood flow in the 17-day-old rat may reflect the energetic and biosynthetic needs of the actively developing brain that are completed by the summation of glucose and ketone body utilization. Reivich, 1974;Sokoloff, 1961Sokoloff, , 1981. Studies performed in immature dogs (Gre goire et aI. , 1978) and in human infants and children (Chugani and Phelps, 1986; Chugani et aI., 1987;Kennedy and Sokoloff, 1957) were also able to show that, in newborns as in adults, cerebral blood flow and metabolism are tightly coupled.The e4C]iodoantipyrine autoradiographic method of Sakurada et al. (1978) requires catheterization of blood vessels in order to permit administration of the tracer and to facilitate collection of timed arte rial blood samples. For these reasons, this tech nique has been mostly used in animals of a quite large size such as adult rats (Ingvar et aI., 1981; McCulloch et aI. , 1982; Mraovitch et aI. , 1986) or c...

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“…The choice of freely diffusible tracer for a given experiment will depend on the following parameters: spatial resolution, invasiveness, the ability to make serial measurc6ments and cost; these are shown in Table 1. In experiments where spatial resolution is more important than the need for serial measurements [14C]iodoantipyrine in conjunction with quantitative autoradiography is appropriate (Reivich et al 1969;Sakurada et al 1978;Richards et al 1987), whereas when the need for serial measurements is paramount, hydrogen clearance methods are suitable. Much of the equipment used for CBF measurement has been designed and built in Southampton.…”

Section: Molten Plastic Inmentioning

confidence: 99%

Proceedings of the Physiological Society, 7‐8 January 1988, Southampton Meeting: Demonstrations

1988

The Journal of Physiology

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A Modification of the Method for the Measurement of Cerebral Blood Flow Using [¹⁴C]Iodoantipyrine in Small Animals

Cited by 16 publications

References 4 publications

Local Cerebral Blood Flow in Rats with Congenital Hydrocephalus

Local Cerebral Blood Flow in Rats with Congenital Hydrocephalus

Postnatal Changes in Local Cerebral Blood Flow Measured by the Quantitative Autoradiographic [¹⁴C]Iodoantipyrine Technique in Freely Moving Rats

Proceedings of the Physiological Society, 7‐8 January 1988, Southampton Meeting: Demonstrations

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A Modification of the Method for the Measurement of Cerebral Blood Flow Using [14C]Iodoantipyrine in Small Animals

Cited by 16 publications

References 4 publications

Local Cerebral Blood Flow in Rats with Congenital Hydrocephalus

Local Cerebral Blood Flow in Rats with Congenital Hydrocephalus

Postnatal Changes in Local Cerebral Blood Flow Measured by the Quantitative Autoradiographic [14C]Iodoantipyrine Technique in Freely Moving Rats

Proceedings of the Physiological Society, 7‐8 January 1988, Southampton Meeting: Demonstrations

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A Modification of the Method for the Measurement of Cerebral Blood Flow Using [¹⁴C]Iodoantipyrine in Small Animals

Postnatal Changes in Local Cerebral Blood Flow Measured by the Quantitative Autoradiographic [¹⁴C]Iodoantipyrine Technique in Freely Moving Rats