Measurement of Postvoid Residual Urine in Conscious Mice Using High-Frequency Transrectal Ultrasound

Hirahara, Naoki; Ukimura, Osamu; Hayashi, Issei; Kamoi, Kazumi; Kawauchi, Akihiro; Matsui, Masanao; Miki, Tsuneharu

doi:10.1016/j.ultrasmedbio.2012.04.012

Cited by 1 publication

(3 citation statements)

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“…Postvoiding residual volume measured by ultrasonography is a very important parameter with which to evaluate bladder function in humans and is frequently examined in clinical practice (18). However, these parameters have rarely been investigated in rodents (12). This is the first report, to our knowledge, on the transabdominal ultrasonographic investigation of bladder dynamics, maximum bladder capacity, and postvoiding residual volume in mice.…”

Section: Usefulness Of Transabdominal Bladder Ultrasonographymentioning

confidence: 98%

“…Furthermore, for the precise and detailed analysis of bladder function, the maximum bladder capacity and postvoiding residual volume should also be examined. Although these parameters are quite important for the examination of human bladder function in clinical practice, there are few reports analyzing these factors in rodents (12). In the present study, we examined the efficacy of transabdominal bladder ultrasonography for analysis of mouse bladder function.…”

mentioning

confidence: 97%

“…Actually, the cystometric voided volume (VV) of the normal mouse varies widely between 29 and 168 l according to previous reports (9,17,23,24). However, these cystometric VVs are remarkably small compared with mouse spontaneous VVs estimated from voided spots on paper (ranging from 200 to 520 l) (12,16,22). It has been suggested that the low VV as measured by mouse cystometry can be attributed to the inhibition of bladder function caused by anesthesia (25), laparotomy, and bladder catheter placement.…”

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confidence: 99%

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Combination of bladder ultrasonography and novel cystometry method in mice reveals rapid decrease in bladder capacity and compliance in LPS-induced cystitis

Takezawa

Kondo

Kiuchi

et al. 2014

American Journal of Physiology-Renal Physiology

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Takezawa K, Kondo M, Kiuchi H, Soda T, Takao T, Miyagawa Y, Tsujimura A, Nonomura N, Shimada S. Combination of bladder ultrasonography and novel cystometry method in mice reveals rapid decrease in bladder capacity and compliance in LPS-induced cystitis. Am J Physiol Renal Physiol 307: F234 -F241, 2014. First published May 7, 2014 doi:10.1152/ajprenal.00043.2014.-Various animal models have been used in research into bladder dysfunction, and in vivo cystometry is a common method to analyze bladder function in animals. However, it is rather difficult to perform reliably in small animals. Transabdominal bladder ultrasonography combined with cystometry in urethane-anesthetized mice have revealed physical inhibition of bladder wall movement by a bladder catheter conventionally placed in the bladder apex. For reliable evaluation of mouse lower urinary tract function, we established a novel cystometry method in which a catheter was placed in the bladder anterior wall, in combination with bladder ultrasonography. This new method allowed the bladder to be well distended (i.e., larger maximum bladder capacity, lower pressure threshold, higher voided volume, and higher bladder compliance compared with conventional methods), which reflected more spontaneous voiding than conventional cystometry methods. We also demonstrated the usefulness of bladder ultrasonography for analysis of mouse bladder function, especially bladder dynamics, maximum bladder capacity, and post-voiding residual volume. We analyzed bladder functional changes in lipopolysaccharide (LPS)-induced cystitis by combining bladder ultrasonography and this new cystometry method. Bladder ultrasonography revealed a rapid decrease in bladder capacity, and cystometry showed a rapid decrease in voided volume due to intravesical LPS instillation. This new cystometry method also revealed a rapid decrease in bladder compliance caused by LPS instillation, which was not detectable by conventional methods. The combination of ultrasonography and the new cystometry method may become a powerful tool for analysis of mouse bladder function and could contribute to the development of new treatments for bladder dysfunction. bladder ultrasonography; cystometry; mouse; LPS; cystitis VARIOUS DISEASES CAUSE BLADDER dysfunction with lower urinary tract symptoms (11). Some diseases, such as overactive bladder, interstitial cystitis, and neurogenic bladder, lead to storage failure, and other diseases, such as underactive bladder and bladder neck construction, lead to voiding failure. Objective evaluation of bladder function is indispensable for accurate diagnosis and development of appropriate treatment strategy for these diseases. Bladder ultrasonography, which can evaluate postvoiding residual volume and bladder wall thickness, is noninvasive and the most common examination for bladder function (5). Cystometry, which can identify detrusor overactivity, low bladder compliance (BCP), and sensory urgency, is an invasive but gold standard examination for bladder function (8). Thus these ex...

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Section: Usefulness Of Transabdominal Bladder Ultrasonographymentioning

confidence: 98%

mentioning

confidence: 97%