Measurement of porcine haptoglobin in meat juice using surface acoustic wave biosensor technology

Klauke, T.; Gronewold, Thomas M. A.; Perpeet, M.; Plattes, Susanne; Petersen, Brigitte

doi:10.1016/j.meatsci.2013.03.028

Cited by 7 publications

(1 citation statement)

References 24 publications

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“…[11] Thorsten N. Klauke et al used commercially available surface acoustic wave devices from SAW Instruments, Germany, to target haptoglobin in porcine meat juice. [12] Chenyun Wang et al used a similar commercially available device from the same, SAW Instruments, Germany, to quantitatively detect exosomes from human blood using mass enhancement from gold nanoparticles (AuNPs). [13] That SAW device was capable of detecting 1.1 x10 3 particles ml -1 , making it potentially suited for liquid biopsies.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Aimofumhe,

Badewole,

Smith

et al. 2024

Preprint

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Abstracts In this article, we describe a simple procedure for evaluating the sterility of nutrient media using a shear-horizontal surface acoustic wave (SH-SAW) biosensor. We used the SH-SAW biosensor platform to monitor the time-dependent phase responses of the material for the media that binds to a monolayer of 3-mercaptopropanyl-N-hydroxy succinimide on the biosensor surface. Increases in the phase shift less than 50% of the starting phase shift recorded 180 minutes after the sterilization indicate a successful sterility test of the media. Our devices were then applied to sterility testing using a viable Escherichia coli (E. coli) K-12 solution to confirm our hypothesis. Filtered LB Broth was the negative control, and a 24-hour culture of E. coli was the positive control. Before testing, the cell solution was pelleted, the media decanted, and then replaced with a 1x phosphate-buffered saline solution (1x PBS). Since we know that the N-hydroxysuccinimide residues will bind to amine groups, only in cases where microbial species are present will the surface binding increase greater than 50%. We confirmed our hypothesis using SH-SAW biosensors and overnight E. coli cultures. The sensitivity for our 250 MHz quartz SH-SAW sensors was calculated based on the response from the E. coli K-12 solution over the linear dynamic range, which appears to be 2x10^4 – 2x10^7 CFU per ml and has a sensitivity of S = 1.655x10^5 o m2 Kg-1. The limit of detection (LOD) for these sensors was calculated to be approximately 2x10^-9 grams (g), which is estimated to be approximately 2,000 Escherichia coli (E. coli) K-12 cells. However, the sensitivity and insertion loss remained approximately unchanged before and after coating the sensor with 3,3'-Dithiobis(succinimidyl) Propionate (DSP).

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Aimofumhe,

Badewole,

Smith

et al. 2024

Preprint

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Proteomic methods for the study of porcine acute phase proteins – anything new to detect?

Miller

Gianazza

2023

Vet Res Commun

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Acute phase proteins (APPs) reflect the health status of individuals and are important tools in diagnostics, as their altered levels are a sign of disturbed homeostasis. While, in most cases, quantitation of known serum APPs is routinely performed by immunoassays, proteomics is helpful in discovery of new biomarker candidates, especially in samples other than body fluids. Besides putting APP regulation into an overall context of differentially abundant proteins, this approach can detect further details or outright new features in protein structure or specific modifications, and help understand better their function. Thus, it can show up ways to make present diagnostic assays more sensitive and/or specific, or correlate regulations of disease-specific proteins. The APP repertoire is dependent on the species. The pig is both, an important farm animal and a model animal for human diseases, due to similarities in physiology. Besides reviewing existing literature, yet unpublished examples for two-dimensional electrophoresis in connection with pig APPs highlight some of the benefits of proteomics. Of further help would be the emerging targeted proteomics, offering the possibility to determine particular isoforms or proteoforms, without the need of specific antibodies, but this method is presently scarcely used in veterinary medicine.

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Meat inspection and hygiene in a Meat Factory Cell – An alternative concept

et al. 2018

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Measurement of porcine haptoglobin in meat juice using surface acoustic wave biosensor technology

Cited by 7 publications

References 24 publications

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media

Proteomic methods for the study of porcine acute phase proteins – anything new to detect?

Meat inspection and hygiene in a Meat Factory Cell – An alternative concept

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