Measurement of paraoxonase (PON) concentrations in coronary heart desease (CHD) subjects by sandwich Elisa

Visible detection of biotin by thin-film interference: thickness control through exchange reaction of biotin/dethiobiotin–avidin bonding

“…Most of the well developed solid phase immunoassays require the use of a second antibody, labeled with a fluorescent agent or enzyme, to give immune information by means of a color change. [1][2][3][4][5] Since the antigen-antibody reaction is highly sensitive, these sensing methods have potential applications in medical and pharmaceutical fields. However, the necessity of second and third reactions required for pigmentation of the system is one of the biggest problems with these methods.…”