Visible detection of biotin by thin-film interference: thickness control through exchange reaction of biotin/dethiobiotin–avidin bonding

Tominaga, Ryojiro; Sivakumar, Muthusamy; Tanaka, Masayoshi; Kinoshita, Takatoshi

doi:10.1039/b715290f

Cited by 11 publications

(5 citation statements)

References 18 publications

(17 reference statements)

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“…This promotes faster binding and the elongated BSA molecule can likely form a thin compact layer by spreading out, thereby interacting with several avidin molecules. By alternating additions of avidin and biotin-BSA, multilayers of proteins could also be assembled (not shown), in agreement with previous work (Tominaga et al, 2008). As a control experiment we also introduced avidin to nanopores treated identically but without the biotinylation step, which showed some non-specific binding to the APTES-modified silicon nitride walls, but considerably slower and weaker (Figure 3D), confirming that biotin is present inside the pores in the other measurements.…”

Section: Resultssupporting

confidence: 91%

Detecting Selective Protein Binding Inside Plasmonic Nanopores: Toward a Mimic of the Nuclear Pore Complex

et al. 2018

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Biosensors based on plasmonic nanostructures offer label-free and real-time monitoring of biomolecular interactions. However, so do many other surface sensitive techniques with equal or better resolution in terms of surface coverage. Yet, plasmonic nanostructures offer unique possibilities to study effects associated with nanoscale geometry. In this work we use plasmonic nanopores with double gold films and detect binding of proteins inside them. By thiol and trietoxysilane chemistry, receptors are selectively positioned on the silicon nitride interior walls. Larger (~150 nm) nanopores are used detect binding of averaged sized proteins (~60 kg/mol) with high signal to noise (>100). Further, we fabricate pores that approach the size of the nuclear pore complex (diameter down to 50 nm) and graft disordered phenylalanine-glycine nucleoporin domains to the walls, followed by titration of karyopherinβ1 transport receptors. The interactions are shown to occur with similar affinity as determined by conventional surface plasmon resonance on planar surfaces. Our work illustrates another unique application of plasmonic nanostructures, namely the possibility to mimic the geometry of a biological nanomachine with integrated optical sensing capabilities.

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Section: Resultssupporting

confidence: 91%

Detecting Selective Protein Binding Inside Plasmonic Nanopores: Toward a Mimic of the Nuclear Pore Complex

et al. 2018

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“…Polymer and organic materials include cholesteric liquid crystals,23 block copolymer24and polymer multilayer by layer‐by‐layer,25 coextrusion,26 and holographic photopolymerization 27. These chemically tunable 1DPCs open up applications in controllable release,28 displays,29 sensors,17–21, 30–32 lasers,33 and so on.…”

Section: Introductionmentioning

confidence: 99%

Bioinspired Water‐Vapor‐Responsive Organic/Inorganic Hybrid One‐Dimensional Photonic Crystals with Tunable Full‐Color Stop Band

Wang

Zhang

Xie

et al. 2010

Adv Funct Materials

181

139

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Bioinspired organic/inorganic hybrid one-dimensional photonic crystals (1DPCs) are prepared by alternating thin fi lms of titania and poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (PHEMA-co-PGMA) by spincoating, which is a simple, reproducible, and low-cost approach. Their optical properties are tuned by changing the number of layers, incident angles, and the thickness of the layers. The color of the 1DPCs can span the entire visible spectral range when the period or the refractive index is changed. Due to the response of PHEMA-co-PGMA to water vapor, the 1DPCs possess fast water-vapor responsiveness and reversible full-color switching. The color of the 1DPCs varies from blue to green, yellow, orange, and red under differing humidities, covering the whole visible range. At high water-vapor concentrations, the color of the 1DPCs rapidly changes from blue to red and comes back to the original state immediately after exposure to air; this behaviour is like that of some animals in nature. The repeatability of the reversible response of the 1DPCs to water vapor is perfect and can be repeated more than 100 times. The as-prepared 1DPCs successfully combine structural color and water-vapor sensitivity, which is promising for use as materials for colorful detection across the full color range.

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“…When interference occurs between the reflected light on the upper and bottom interface of a thin film substrate, the interference color is determined by the thickness and refractive index. Therefore, by controlling the thickness via the surface binding activities of analytes, the color change can be achieved and used for detection [ 59 ].…”

Section: Fundamentals Of Photonic Biosensorsmentioning

confidence: 99%

Progress and Challenges of Point-of-Need Photonic Biosensors for the Diagnosis of COVID-19 Infections and Immunity

Liu

Wachsmann‐Hogiu

2022

Biosensors

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The new coronavirus disease, COVID-19, caused by SARS-CoV-2, continues to affect the world and after more than two years of the pandemic, approximately half a billion people are reported to have been infected. Due to its high contagiousness, our life has changed dramatically, with consequences that remain to be seen. To prevent the transmission of the virus, it is crucial to diagnose COVID-19 accurately, such that the infected cases can be rapidly identified and managed. Currently, the gold standard of testing is polymerase chain reaction (PCR), which provides the highest accuracy. However, the reliance on centralized rapid testing modalities throughout the COVID-19 pandemic has made access to timely diagnosis inconsistent and inefficient. Recent advancements in photonic biosensors with respect to cost-effectiveness, analytical performance, and portability have shown the potential for such platforms to enable the delivery of preventative and diagnostic care beyond clinics and into point-of-need (PON) settings. Herein, we review photonic technologies that have become commercially relevant throughout the COVID-19 pandemic, as well as emerging research in the field of photonic biosensors, shedding light on prospective technologies for responding to future health outbreaks. Therefore, in this article, we provide a review of recent progress and challenges of photonic biosensors that are developed for the testing of COVID-19, consisting of their working fundamentals and implementation for COVID-19 testing in practice with emphasis on the challenges that are faced in different development stages towards commercialization. In addition, we also present the characteristics of a biosensor both from technical and clinical perspectives. We present an estimate of the impact of testing on disease burden (in terms of Disability-Adjusted Life Years (DALYs), Quality Adjusted Life Years (QALYs), and Quality-Adjusted Life Days (QALDs)) and how improvements in cost can lower the economic impact and lead to reduced or averted DALYs. While COVID19 is the main focus of these technologies, similar concepts and approaches can be used and developed for future outbreaks of other infectious diseases.

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Visible detection of biotin by thin-film interference: thickness control through exchange reaction of biotin/dethiobiotin–avidin bonding

Cited by 11 publications

References 18 publications

Detecting Selective Protein Binding Inside Plasmonic Nanopores: Toward a Mimic of the Nuclear Pore Complex

Detecting Selective Protein Binding Inside Plasmonic Nanopores: Toward a Mimic of the Nuclear Pore Complex

Bioinspired Water‐Vapor‐Responsive Organic/Inorganic Hybrid One‐Dimensional Photonic Crystals with Tunable Full‐Color Stop Band

Progress and Challenges of Point-of-Need Photonic Biosensors for the Diagnosis of COVID-19 Infections and Immunity

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