Measurement of non-electrolyte concentrations in an ion solution using a micro-electrode sensor

Isoda, Takaaki; Takahara, Naoki; Imanaga, Hiroki; Imamura, Ryosuke; Hasegawa, S.; Noguchi, K.; Kimura, Taro

doi:10.1016/j.snb.2006.01.039

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…The preparation and application of microelectrodes have attracted much interest in recent analytical chemistry studies [39]. This is part of a general trend in analytical chemistry towards miniaturization.…”

Section: Resultsmentioning

confidence: 99%

Microsized Graphite Sensors for Potentiometric Determination of Metronidazole and Spiramycin

Khattab¹,

Ramadan²,

Hegazy³

et al. 2011

Port. Electrochim. Acta

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Two microsized graphite-design sensors based on ionophore technique, polyvinyl chloride carboxylated (PVC-COOH) and β-cyclodextrin (β-CD), are used for fabrication of two membrane sensors for the two studied drugs, metronidazole (MZ), sensor 1, and spiramycin (SP), sensor 2. Fast and stable Nernstian responses near 1x10-5-1x10-3 M for MZ and 1x10-5-1x10-2 M for SP over pH range 5.5-7.5 for the two electrodes reveal the performance characteristics of these electrodes which have been evaluated according to IUPAC recommendations. The aim of this work is to develop a new, simple, accurate and precise method for the determination of MZ and SP in their binary mixtures, which can be applied in routine quality control. The method is successively applied for the determination of the two drugs in their pharmaceutical formulations. Validation of the method according to the quality assurance standards shows suitability of the proposed electrodes for the use in the quality control assessment of these drugs. The recovery percentages for the determination of the two drugs by the two proposed selective electrodes are 99.86 ± 0.249 % and 99.69 ± 0.856% for sensors 1 and 2, respectively. Statistical comparison between the results obtained by this method and the reported one is done and no significant difference is found.

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Section: Resultsmentioning

confidence: 99%

Microsized Graphite Sensors for Potentiometric Determination of Metronidazole and Spiramycin

Khattab¹,

Ramadan²,

Hegazy³

et al. 2011

Port. Electrochim. Acta

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show abstract

“…The molecular weight of SPG was about 150,000 and its end unit was modified with an amino group. The amino group of SPG bound covalently to a carboxyl group on the support surface to form the composite PS∙SPG [ 8 ]. Nucleic acids were preferentially adsorbed in particular, and could be detected by the microelectrode when the adsorbent consisted of PS microbeads with a SPG-modified surface.…”

Section: Introductionmentioning

confidence: 99%

Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped with Silica Particles and Fluorescent Compounds

Isoda

Maeda

2012

JFB

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Biopolymers are easily denatured by heating, a change in pH or chemical substances when they are immobilized on a substrate. To prevent denaturation of biopolymers, we developed a method to trap a polynucleotide on a substrate by hydrogen bonding using silica particles with surfaces modified by aminoalkyl chains ([A-AM silane]/SiO2). [A-AM silane]/SiO2 was synthesized by silane coupling reaction of N-2-(aminoethyl)-3-aminopropyltrimethoxysilane (A-AM silane) with SiO2 particles with a diameter of 5 μm at 100 °C for 20 min. The surface chemical structure of [A-AM silane]/SiO2 was characterized by Fourier transform infrared spectroscopy and molecular orbital calculations. The surface of the silica particles was modified with A-AM silane and primary amine groups were formed. [A-AM silane]/SiO2 was trapped with single-stranded nucleic acids [(Poly-X; X = A (adenine), G (guanine) and C (cytosine)] in PBS solution at 37 °C for 1 h. The single-stranded nucleic acids were trapped on the surface of the [A-AM silane]/SiO2 by hydrogen bonding to form conjugated materials. The resulting complexes were further conjugated by derivatives of acridine orange (AO) as fluorescent labels under the same conditions to form [AO:Poly-X:A-AM silane]/SiO2 complexes. Changes in the fluorescence intensity of these complexes originating from interactions between the single-stranded nucleic acid and aromatic compounds were also evaluated. The change in intensity displayed the order [AO: Poly-G: A-AM silane]/SiO2 > [AO:Poly-A:A-AM silane]/SiO2 >> [AO:Poly-C:A-AM silane]/SiO2. This suggests that the single-stranded nucleic acids conjugated with aminoalkyl chains on the surfaces of SiO2 particles and the change in fluorescence intensity reflected the molecular interaction between AO and the nucleic-acid base in a polynucleotide.

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A vivid Au-porous anodic alumina composite film with the inverted taper structure for label-free detection

Zhao

et al. 2023

Nano Res.

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Measurement of non-electrolyte concentrations in an ion solution using a micro-electrode sensor

Cited by 6 publications

References 27 publications

Microsized Graphite Sensors for Potentiometric Determination of Metronidazole and Spiramycin

Microsized Graphite Sensors for Potentiometric Determination of Metronidazole and Spiramycin

Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped with Silica Particles and Fluorescent Compounds

A vivid Au-porous anodic alumina composite film with the inverted taper structure for label-free detection

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