Measurement of lumefantrine and its metabolite in plasma by high performance liquid chromatography with ultraviolet detection

Khalil, Insaf F.; Abildrup, Ulla; Alifrangis, Lene; Maiga, Deogratius; Alifrangis, Michael; Hoegberg, Lotte Christine Groth; Vestergaard, Lasse S; Persson, Ola Per-Eric; Nyagonde, Nyagonde; Lemnge, Martha; Theander, Thor G.; Bygbjerg, Ib Christian

doi:10.1016/j.jpba.2010.08.009

Cited by 18 publications

(8 citation statements)

References 9 publications

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“…A nested PCR method was used to analyse polymorphisms in the genes coding for the merozoite surface protein (MSP) 1 and 2 to distinguish between recrudescent and new infections [23, 24]. The plasma concentrations of amodiaquine and its metabolites (desethylamodiaquine; bis-desethylamodiaquine) and lumefantrine and its metabolites (desbutyllumefantrine) were measured by means of a high performance liquid chromatographic (HPLC) method [25]. The SCD or HbAA status of recruited subjects was determined by a sodium metabisulphite test and alkaline haemoglobin electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

A randomized trial of artesunate-amodiaquine versus artemether-lumefantrine in Ghanaian paediatric sickle cell and non-sickle cell disease patients with acute uncomplicated malaria

Adjei

Goka

Enweronu‐Laryea

et al. 2014

Malar J

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BackgroundSickle cell disease (SCD) is a genetic disorder common in malaria endemic areas. In endemic areas, malaria is a major cause of morbidity and mortality among SCD patients. This suggests the need for prompt initiation of efficacious anti-malarial therapy in SCD patients with acute malaria. However, there is no information to date, on the efficacy or safety of artemisinin combination therapy when used for malaria treatment in SCD patients.MethodsChildren with SCD and acute uncomplicated malaria (n = 60) were randomized to treatment with artesunate-amodiaquine (AA), or artemether-lumefantrine (AL). A comparison group of non-SCD children (HbAA genotype; n = 59) with uncomplicated malaria were also randomized to treatment with AA or AL. Recruited children were followed up and selected investigations were done on days 1, 2, 3, 7, 14, 28, 35, and 42. Selected clinical and laboratory parameters of the SCD patients were also compared with a group of malaria-negative SCD children (n = 82) in steady state.ResultsThe parasite densities on admission were significantly lower in the SCD group, compared with the non-SCD group (p = 0.0006). The parasite reduction ratio (PRR) was lower, clearance was slower (p < 0.0001), and time for initial parasitaemia to decline by 50 and 90% were longer for the SCD group. Adequate clinical and parasitological response (ACPR) on day 28 was 98.3% (58/59) in the SCD group and 100% (57/57) in the non-SCD group. Corresponding ACPR rates on day 42 were 96.5% (55/57) in the SCD group and 96.4% (53/55) in the non-SCD group. The fractional changes in haemoglobin, platelets and white blood cell counts between baseline (day 0) and endpoint (day 42) were 16.9, 40.6 and 92.3%, respectively, for the SCD group, and, 12.3, 48.8 and 7.5%, respectively, for the non-SCD group. There were no differences in these indices between AA- and AL-treated subjects.ConclusionsThe parasite clearance of SCD children with uncomplicated malaria was slower compared with non-SCD children. AA and AL showed similar clinical and parasitological effects in the SCD and non-SCD groups. The alterations in WBC and platelet counts may have implications for SCD severity.Trial registrationCurrent controlled trials ISRCTN96891086.

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Section: Methodsmentioning

confidence: 99%

A randomized trial of artesunate-amodiaquine versus artemether-lumefantrine in Ghanaian paediatric sickle cell and non-sickle cell disease patients with acute uncomplicated malaria

Adjei

Goka

Enweronu‐Laryea

et al. 2014

Malar J

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“…Due to technical limitations including laboriously lengthy extraction process and low sensitivity, earlier high-performance liquid chromatography methods are not adequate for follow up of low LUM levels in small volume samples [126,182,184,185]. Recently, more sensitive liquid chromatography tandem mass spectrometry techniques have been published [186][187][188].…”

Section: Challenges In Quantification Of Lumefantrine In Bloodmentioning

confidence: 99%

A fast and sensitive method for quantifying lumefantrine and desbutyl-lumefantrine using LC–MS/MS

Silva

Mwebaza

Ntale

et al. 2015

Journal of Chromatography B

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“…Thus, a rapid, sensitive, and specific analytical method for the simultaneous determination of LUM, ARM, and its active metabolite dihydroartemisinin (DHA) in biological fluids is urgently needed for evaluating the bioavailability, pharmacokinetic, and safety profile of this combination of medicines. Historically, while the HPLC method coupled with UV detection for the quantitative determination of LUM has achieved a lower limit of quantification (LLOQ) of 12.5 ng/mL [4], the use of this method for analysis of ARM and DHA is difficult, in part due to the lack of strong UV chromophores in artemisinin derivatives ( Figure 1). Mannemala et al reported an LC method using diode array detection for the determination of five antimalarial drugs, but with higher LLOQ values of 300 and 30 ng/mL for ARM and LUM in human plasma, respectively [5].…”

Section: Introductionmentioning

confidence: 99%

A sensitive, high‐throughput, and ecofriendly method for the determination of lumefantrine, artemether, and its active metabolite dihydroartemisinin by supercritical fluid chromatography and tandem mass spectrometry

Yang

Gao

Hou

et al. 2018

J of Separation Science

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A quick and sensitive supercritical fluid chromatography with tandem mass spectrometry method for the simultaneous determination of lumefantrine, artemether, and its active metabolite dihydroartemisinin in rat plasma was developed and validated. The chromatographic separation was performed on an ACQUITY UPC ™ BEH 2-EP column within 2.5 min by gradient elution using compressed CO and methanol containing 2 mM ammonium acetate as the mobile phases. Detection was achieved by multiple reaction monitoring using electrospray ionization in the positive ionization mode. For sample preparation, 50 μL of the sample was processed by modified high-throughput, one-step protein precipitation using hydrogen peroxide as a stabilizer to protect the endoperoxide-containing artemisinin derivatives from degradation. The calibration curves were linear over the concentration range of 2.0-1000 ng/mL for both artemether and dihydroartemisinin, and 1.0-5000 ng/mL for lumefantrine. The values of selectivity, lower limit of quantification, linearity, accuracy, precision, matrix effects, stability, and recovery met the acceptable range according to the Food and Drug Administration guidelines. The developed method enables high resolution and speed as well as low cost, low solvent consumption, and short time and was successfully applied to pharmacokinetic studies through the intravenous administration of an artemether-lumefantrine lipid emulsion in rats.

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Measurement of lumefantrine and its metabolite in plasma by high performance liquid chromatography with ultraviolet detection

Cited by 18 publications

References 9 publications

A randomized trial of artesunate-amodiaquine versus artemether-lumefantrine in Ghanaian paediatric sickle cell and non-sickle cell disease patients with acute uncomplicated malaria

A randomized trial of artesunate-amodiaquine versus artemether-lumefantrine in Ghanaian paediatric sickle cell and non-sickle cell disease patients with acute uncomplicated malaria

A fast and sensitive method for quantifying lumefantrine and desbutyl-lumefantrine using LC–MS/MS

A sensitive, high‐throughput, and ecofriendly method for the determination of lumefantrine, artemether, and its active metabolite dihydroartemisinin by supercritical fluid chromatography and tandem mass spectrometry

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