Measurement of leptin in dried blood spot samples

Miller, Aaron A.; Sharrock, Katherine C.; McDade, Thomas W.

doi:10.1002/ajhb.20566

Cited by 20 publications

(19 citation statements)

References 7 publications

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“…We did, however, find that DBS values were consistently lower than serum or plasma values. Similar differences in serum or plasma versus DBS levels have been reported previously for CRP and other analytes ((McDade and Shell-Duncan, 2002; McDade et al, 2004; Miller et al, 2006; Baingana et al, 2008) and others). These differences might stem from inaccuracy in the estimate of sample volume contained in DBS punches (Mei et al, 2001), or might indicate incomplete elution of the CRP from the paper.…”

Section: Discussionsupporting

confidence: 87%

Serum, plasma, and dried blood spot high-sensitivity C-reactive protein enzyme immunoassay for population research

Brindle

Fujita

Shofer

et al. 2010

Journal of Immunological Methods

143

119

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C-reactive protein (CRP) is used as a biomarker of morbidity and mortality risk in studies of population health, and is essential to interpretation of several micronutrient biomarkers. There is thus need for a robust high sensitivity CRP (hsCRP) measurement method for large-scale, nonclinical studies. We developed an efficient, inexpensive assay suitable for quantifying CRP across the physiological range using any blood specimen type. The ELISA uses readily available monoclonal antibodies to measure CRP in serum, plasma, or dried blood spots (DBS) made from venous or capillary blood. Assay performance was evaluated by standard methods, including comparison with a previously described assay. Effects of specimen type were tested by measuring CRP in 52 matched serum, plasma, and venous and capillary dried blood spot specimens. Longand short-term CRP stability were evaluated. Assessments of assay limits of detection, linearity, recovery, imprecision, and concordance with an established method (Pearson correlation = 0.988, n = 20) demonstrated the validity of the new assay. CRP measurements in serum, plasma, and DBS had Pearson correlations from 0.974 to 0.995, n = 52, but CRP in serum was on average 1.6 times (SD 0.37) higher than in DBS. CRP was stable in frozen serum for up to 34 months, but DBS CRP declined quickly with exposure to ambient temperatures, and across long-term storage at −20°C. This hsCRP assay is a robust and inexpensive tool designed for use in large-scale population health research. Our results indicate that DBS CRP is less stable than previously reported.

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Section: Discussionsupporting

confidence: 87%

Serum, plasma, and dried blood spot high-sensitivity C-reactive protein enzyme immunoassay for population research

Brindle

Fujita

Shofer

et al. 2010

Journal of Immunological Methods

143

119

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“…Proteins in blood spots are beyond detection in a matter of days [4], but nucleic acids show evidence of long-term stability. The published literature shows extensive examples of recovery and analysis of nucleic acids from dried blood spots.…”

Section: Introductionmentioning

confidence: 99%

Archived Unfrozen Neonatal Blood Spots Are Amenable to Quantitative Gene Expression Analysis

et al. 2008

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Background: State laws in the USA mandate that blood be drawn from all newborn infants to screen for health-threatening conditions. These screening assays consume only a small portion of the blood samples, which are collected on filter paper (‘Guthrie’) cards. Many states archive unused blood spots, often in unrefrigerated storage. Objectives: While individual RNA transcripts have been identified from archived neonatal blood spots, no study to date has performed quantitative analysis of archived blood spot RNA. Methods: We demonstrate that RNA can be isolated and amplified from newborn blood spots stored unfrozen for as long as 9 years, and can be analyzed by microarray and qPCR. Results: Microarray assays of archived neonatal blood spots consistently detected 3,000–4,000 expressed genes with correlations of 0.90 between replicates. Blood spot mRNA is amenable to qPCR and we detected biologically relevant expression levels of housekeeping and immune-mediating genes. Conclusions: These experiments demonstrate the feasibility of using blood spots as a source of RNA which can be analyzed using quantitative microarray and qPCR assays. The application of these methods to the analysis of widely collected biological specimens may be a valuable resource for the study of perinatal determinants of disease development.

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“…This is an expanding area of research as assays for more analytes are currently being developed 1 . Among the assays already validated and being widely used are cholesterol 2 ,CRP 10,14,15 , IgE16, EBV 9,15 , leptin 17 , and cyotkines 18 . There are a number of laboratories specializing in the assay of DBS, including the University of Washington Department of Laboratory Medicine (UW Lab Med) Biomarker Laboratory (Director: Mark Wener).…”

Section: Freezer Storagementioning

confidence: 99%

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Ostler¹,

Porter

Buxton³

2014

JoVE

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Biomarkers are directly-measured biological indicators of disease, health, exposures, or other biological information. In population and social sciences, biomarkers need to be easy to obtain, transport, and analyze. Dried Blood Spots meet this need, and can be collected in the field with high response rates. These elements are particularly important in longitudinal study designs including interventions where attrition is critical to avoid, and high response rates improve the interpretation of results. Dried Blood Spot sample collection is simple, quick, relatively painless, less invasive then venipuncture, and requires minimal field storage requirements (i.e. samples do not need to be immediately frozen and can be stored for a long period of time in a stable freezer environment before assay). The samples can be analyzed for a variety of different analytes, including cholesterol, C-reactive protein, glycosylated hemoglobin, numerous cytokines, and other analytes, as well as provide genetic material. DBS collection is depicted as employed in several recent studies.

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Measurement of leptin in dried blood spot samples

Cited by 20 publications

References 7 publications

Serum, plasma, and dried blood spot high-sensitivity C-reactive protein enzyme immunoassay for population research

Serum, plasma, and dried blood spot high-sensitivity C-reactive protein enzyme immunoassay for population research

Archived Unfrozen Neonatal Blood Spots Are Amenable to Quantitative Gene Expression Analysis

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

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