Serum, plasma, and dried blood spot high-sensitivity C-reactive protein enzyme immunoassay for population research

Brindle, Eleanor; Fujita, Masako; Shofer, Jane B.; O’Connor, Kathleen

doi:10.1016/j.jim.2010.09.014

Cited by 144 publications

(126 citation statements)

References 28 publications

(34 reference statements)

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“…CRP was measured using a particle enhanced immunonepholometric assay (BNII nephelometer from Dade Behring Inc., Deerfield, IL) at the University of Vermont (Ryff et al, 2013). CRP measured from blood spots are highly correlated with CRP measured from serum (e.g., Brindle et al, 2010). …”

Section: Methodsmentioning

confidence: 99%

Five-factor model personality traits and inflammatory markers: New data and a meta-analysis

Luchetti¹,

Barkley²,

Stéphan³

et al. 2014

Psychoneuroendocrinology

138

114

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The purpose of this research is to examine the association between five major dimensions of personality and systemic inflammation through (a) new data on C-reactive protein (CRP) from three large national samples of adults that together cover most of the adult lifespan and (b) a meta-analysis of published studies on CRP and interleukin-6 (IL-6). New data (total N=26,305) were drawn from the National Longitudinal Study of Adolescent Health, the Midlife in the United States study, and the Health and Retirement Study. PRISMA guidelines were used for the meta-analysis to combine results of up to seven studies on CRP (N=34,067) and six on IL-6 (N=7,538). Across the three new samples, higher Conscientiousness was associated with lower CRP. The Conscientiousness-CRP relation was virtually identical controlling for smoking; controlling for body mass index attenuated this association but did not eliminate it. Compared to participants in the highest quartile of Conscientiousness, participants in the lowest quartile had an up to 50% increased risk of CRP levels that exceeded the clinical threshold (≥3mg/l). The meta-analysis supported the association between Conscientiousness and both CRP and IL-6 and also suggested a negative association between Openness and CRP; no associations were found for Neuroticism, Extraversion and Agreeableness. The present work indicates a modest, but consistent, association between Conscientiousness and a more favorable inflammatory profile, which may contribute to the role of Conscientiousness in better health across the lifespan.

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Section: Methodsmentioning

confidence: 99%

Five-factor model personality traits and inflammatory markers: New data and a meta-analysis

Luchetti¹,

Barkley²,

Stéphan³

et al. 2014

Psychoneuroendocrinology

138

114

View full text Add to dashboard Cite

show abstract

“…Serum was collected and stored at -80°C until it could be analyzed as a batch, to avoid interassay variability. Samples were stored for up to 10 months (CRP concentrations reportedly remain stable in serum stored at -20°C for up to a year in a controlled laboratory setting and up to 34 months in a research setting 28 ). The CRP concentrations were measured with an automated turbidimetric immunoassay for human CRP d,e previously validated for use in dogs.…”

Section: Small Animals/ Exoticmentioning

confidence: 99%

Evaluation of the use of serum C-reactive protein concentration to predict outcome in puppies infected with canine parvovirus

McClure¹,

Schoor²,

Thompson³

et al. 2013

javma

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Objective—To evaluate associations of serum C-reactive protein (CRP) concentration with duration of hospitalization and with outcome in puppies with canine parvoviral enteritis. Design—Prospective observational study. Animals—79 client-owned puppies with naturally acquired canine parvovirus infection. Procedures—All puppies received supportive care. Serum CRP concentration was measured at the time of admission, approximately every 10 to 12 hours for the first 48 hours, and then every 24 hours until discharge from the hospital or death. Associations between outcome and CRP concentration at various time points or changes in CRP concentration over time were assessed via multiple logistic regression. Associations of CRP concentration with survival time and duration of hospitalization among survivors were estimated with Cox proportional hazards regression. Use of CRP concentration to predict outcome was evaluated by means of receiver operating characteristic curve analysis. Results—Serum CRP concentrations at admission and 12 and 24 hours later were positively associated with odds of death, and CRP concentrations at 12 and 24 hours after admission were negatively associated with survival time for puppies. Among survivors, duration of hospitalization was positively associated with CRP concentrations at 12, 24, and 36 hours after admission. Sensitivity and specificity of CRP concentration to differentiate between survivors and nonsurvivors at 24 hours after admission were 86.7% and 78.7%, respectively (considered moderately accurate). Conclusions and Clinical Relevance—Although serum CRP concentration was associated with outcome in puppies with canine parvovirus enteritis, it did not prove to be a good predictor of outcome when used alone.

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“…These drying measures decrease sample weight by >90%, thus decreasing transport burden, and in theory can enhance long-term sample stability by decreasing water-dependent analyte degradation caused by hydrolysis and enzyme activity (12). Unfortunately, DBS stored in challenging environments (i.e., elevated temperatures and humidity) quickly lose their protective capacity (13,14). Indeed, years of field research supported by the Centers for Disease Control has shown that DBS on-card drying approaches do not fully safeguard all blood analytes (15).…”

mentioning

confidence: 99%

Silk-based blood stabilization for diagnostics

Kluge

Kahn

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Advanced personalized medical diagnostics depend on the availability of high-quality biological samples. These are typically biofluids, such as blood, saliva, or urine; and their collection and storage is critical to obtain reliable results. Without proper temperature regulation, protein biomarkers in particular can degrade rapidly in blood samples, an effect that ultimately compromises the quality and reliability of laboratory tests. Here, we present the use of silk fibroin as a solid matrix to encapsulate blood analytes, protecting them from thermally induced damage that could be encountered during nonrefrigerated transportation or freeze-thaw cycles. Blood samples are recovered by simple dissolution of the silk matrix in water. This process is demonstrated to be compatible with a number of immunoassays and provides enhanced sample preservation in comparison with traditional air-drying paper approaches. Additional processing can remediate interactions with conformational structures of the silk protein to further enhance blood stabilization and recovery. This approach can provide expanded utility for remote collection of blood and other biospecimens empowering new modalities of temperature-independent remote diagnostics.lood contains a variety of proteins, enzymes, lipids, metabolites, and peptides, which can be interrogated as biomarkers for health screening, monitoring, and diagnostics. The integrity of these blood components and thus the quality of information attained from their analysis is determined by the storage conditions from sampling until analysis, or the so-called pre-analytical phase (1, 2). This phase often includes time-consuming processing steps and the requirement for a continuous cold storage. Without temperature regulation, blood-derived biospecimens degrade quickly, accounting for up to 67% of all laboratory testing errors (2, 3). Further, when blood-derived materials are frozen, decreases in thermodynamic free energy and unfavorable ice crystal-protein interactions (4) can occur during subsequent thawing (5, 6), which can further compromise analyte integrity as a gold-standard methodology.As an alternative route, blood and blood derivatives can be dried via newer approaches such as isothermal vitrification (7), lyophilization (8), or on silica chips (9). Isothermal vitrification and lyophilization are inherently resource-intensive techniques and thus not suitable for field use. Silica chips are designed for enrichment of selected fractions of the low-molecular-weight serum proteome, but are not broadly protective at elevated temperature (10). An inexpensive alternative used since the 1960s are dried blood spots (DBS) (11), a paper card system which captures blood components among cellulose fibers as the water phase evaporates. These drying measures decrease sample weight by >90%, thus decreasing transport burden, and in theory can enhance long-term sample stability by decreasing water-dependent analyte degradation caused by hydrolysis and enzyme activity (12). Unfortunately, DBS stored in ...

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Serum, plasma, and dried blood spot high-sensitivity C-reactive protein enzyme immunoassay for population research

Cited by 144 publications

References 28 publications

Five-factor model personality traits and inflammatory markers: New data and a meta-analysis

Five-factor model personality traits and inflammatory markers: New data and a meta-analysis

Evaluation of the use of serum C-reactive protein concentration to predict outcome in puppies infected with canine parvovirus

Silk-based blood stabilization for diagnostics

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