Measurement of leaf lamina moisture with a low-cost electrical humidity sensor: case study on a wheat water-mutant

Rascio, Agata; Rinaldi, Michele; Santis, Giuditta De; Pecchioni, Nicola; Palazzo, Gabriele; Palazzo, Nicola

doi:10.1186/s12870-019-1987-4

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…Similarly, leaf water content is one of the regulatory physiological parameters that limit the efficiency of photosynthesis and biomass production in plants . Also, water content on leaf surfaces is an essential parameter in determination of plant drought and salinity tolerance .…”

Section: Results and Discussionmentioning

confidence: 99%

Moisture-Sensitive Ratiometric Color-Changing Response: a Useful Tool for Precision Farming

Dey

Fernandes

2022

ACS Agric. Sci. Technol.

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Anthra[1,2-d]imidazole-6,11-dione-based charge transfer probes have been developed for the detection of water impurities in a wide range of laboratory-used organic solvents. The deprotonated (anionic) forms of the probe molecules, obtained upon addition of basic anions such as fluoride, demonstrate a ratiometric color-changing response (blue to yellow in presence of F– and red to yellow with CN– ion) even in the presence of a trace amount of moisture (detection limit: 0.008 wt %). Considering their high sensitivity and “naked-eye” response, the anionic receptors are utilized for the quantification of moisture level or water impurity in a wide range of real-life samples, such as building raw materials, packaged food items, soil, plant leaves, and so on. Determination of the moisture level of plant leaves or soil samples is particularly important for precision farming as it helps the farmers to schedule the irrigation events. Finally, low-cost, reusable, and optical kit-based dye-coated paper strips were developed for rapid, on-location detection as well as quantification purposes. Since such a sustainable approach does not involve any sophisticated instruments or complex sample preparation steps, the general public with little knowledge of science or technology will be able to use it without much difficulty.

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Section: Results and Discussionmentioning

confidence: 99%

Moisture-Sensitive Ratiometric Color-Changing Response: a Useful Tool for Precision Farming

Dey

Fernandes

2022

ACS Agric. Sci. Technol.

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“…This Water-Mutant has a very negative hydration enthalpy of bound water, while the water fraction is essential for the structural integrity of biomolecules [ 30 ] that might also exert passive control of the osmotically active volume of the cell [ 31 ]. All of the genotypes were sown indoors (as described below) and in 10-m 2 large plots of the experimental fields of CREA-CI, of Foggia (Italy), as previously reported [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

A Low-Cost Method for Phenotyping Wilting and Recovery of Wheat Leaves under Heat Stress Using Semi-Automated Image Analysis

Rascio

Santis

Sorrentino

2020

Plants

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Leaf wilting is the most common symptom of dehydration stress. Methods to analyze this phenomenon are particularly relevant to evaluate crop agronomic performance, to genetically dissect out the wilting process, and for functional analysis of genetically modified plants. In this study, a low-cost, semi-automated method to quantify leaf folding of wilting plants is described that can replace visual analysis. Standardized heat-stress conditions were applied with a thermostatic drier, on plantlets or excised leaves from three wheat genotypes (Trinakria, Cappelli, and a Water-mutant of Trinakria). The best time–temperature binomial to record both the leaf wilting and recovery phases was identified using a free time-lapse application, by a smartphone camera. The quantitative description of the wilting phenomenon was obtained through the Kinovea software, which automatically tracked the leaf angle changes over time, computed various kinematic data (angular velocity, centripetal acceleration, total degrees of displacement) and constructed the graphs. The possibility of applying standardized heat-stress conditions and quantitatively describe the leaf folding kinematics means that this instrumentation and its use represents a very low cost tool for objective phenotyping of the degree of the heat-stress tolerance of wheat and of morphologically similar species.

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“…Additionally, the capacitance sensor has the advantages of high measurement accuracy and non-invasiveness. Using the capacitive method to measure the dielectric constant of the leaf, the leaf is not damaged and continuous measurement of the leaf-holding capacity and water content can be ensured [26,27]. Therefore, in this study, we investigate the law of the effect of this change on electrostatic spraying technology by changing the leaf water content, causing a change in leaf capacitance.…”

Section: Introductionmentioning

confidence: 99%

Influence of Plant Leaf Moisture Content on Retention of Electrostatic-Induced Droplets

Liu

Chen

et al. 2021

Sustainability

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Agricultural electrostatic spraying can help to reduce the threat of pesticides to human health and the environment. However, the influence of the law of leaf water content on electrostatic spraying has not been studied. In this study, we used leaf water content as an evaluation index of electrostatic spraying technology and verified the correlation between leaf water content and leaf capacitance value by statistical methods in order to achieve in vivo measurements of leaf water content in relation to tomato, pepper, and wheat crop leaves. Using these in vivo measurements of leaf water content and retention, we demonstrate that the retention of electrostatic droplets on the leaves of all three crops increases with increasing water content; the retention per unit area of leaves increased by 6.1 mg/cm2, an increase of 7.29%. Increasing the electrostatic spray voltage (10~30 kV) enhances the retention of droplets on the leaves of the crops, with a maximum increase of 6.1. The retention of non-electrostatic droplets decreases with increasing water content; retention at the lowest water content was 1.103~1.131 times greater than at the highest water content. This study has implications for research related to improving the retention of electrostatic droplets in leaves.

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Measurement of leaf lamina moisture with a low-cost electrical humidity sensor: case study on a wheat water-mutant

Cited by 11 publications

References 22 publications

Moisture-Sensitive Ratiometric Color-Changing Response: a Useful Tool for Precision Farming

Moisture-Sensitive Ratiometric Color-Changing Response: a Useful Tool for Precision Farming

A Low-Cost Method for Phenotyping Wilting and Recovery of Wheat Leaves under Heat Stress Using Semi-Automated Image Analysis

Influence of Plant Leaf Moisture Content on Retention of Electrostatic-Induced Droplets

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