Measurement of IgE antibodies against purified grass pollen allergens (Lol p 1, 2, 3 and 5) during immunotherapy

Abstract

Measurement of IgE antibodies against purified grass pollen allergens (Lol p 1, 2, 3 and 5) during immunotherapy van Ree, R.; Leeuwen, W.A.; Dieges, P.H.; van Wijk, R.G.; de Jong, N.; Brewczyski, P.Z.; Kroon, A.M.; Schilte, P.P.M.; Tan, K.Y.; Simon-Licht, I.F.; Roberts, A.M.; Stapel, S.; Aalberse, R.C. Published in:Clinical and Experimental Allergy DOI:10.1046/j. 1365-2222.1997.d01-416.x Link to publication Citation for published version (APA): van Ree, R., Leeuwen, W. A., Dieges, P. H., van Wijk, R. G., de Jong, N., Brewczyski, P. Z., ... Aalberse, R. C. (1997). Measurement of IgE antibodies against purified grass pollen allergens (Lol p 1, 2, 3 and 5) during immunotherapy. Clinical and Experimental Allergy, 27, 68-74. https://doi.org/10.1046/j. 1365-2222.1997.d01-416.x General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Download date: 22 Mar 2019Cliniecd and Experimenhd Allergy. 1997, Volume 27, pages 68-74 Measurement of IgE antibodies against purified grass pollen allergens (Lol p 1, 2, 3 and 5) during immunotherapy R. VAN SummaryBackground IgE titres tend to rise early after the start of immunotherapy, followed by a decline to pre-immunotherapy levels or lower. Objectives We were interested to ktiow whether the early increase in IgE antibodies includes new specificities of IgE, and whether these responses persist. Methods Sera of 64 patients undergoing grass pollen immunotherapy were tested for IgE against four purified grass pollen allergens: Lol p 1. 2, 3, and 5. At least two serum samples were taken, one before the start of therapy and one between 5 and 18 months after the first immunization (mean: 10 months). Results The mean IgE responses to Lol p 1,2 and 3 showed a moderate but not significant increase. In contrast, the mean IgE response to Lol p 5 showed a significant decrease of >30%. IgE against total Lolium perenne pollen extract moderately increased (>20%), showing that a RAST for total pollen is not always indicative for the development of IgE against its major allergens. For >A{)% of the patients it was found that IgE against one or more of the four allergens increased, while IgE against the remaining allergen(s) decreased. Eor 10 sera the ratio of IgE titres against at least two allergens changed by at least a factor of 5. The changes ...

Mechanisms of specific immunotherapy of allergic diseases

“…A certain reevaluation of the previous findings might be necessary, since the radioallergosorbent test (RAST) for the whole allergen may not always indicate the development of IgE against particular major allergens. It has recently been reported that SIT with grass-pollen extract induced variable changes (both increases and decreases) in serum specific IgE levels against four purified (major) allergens of Lolium perenne (Lol p 1, 2, 3, and 5), changes which were poorly correlated with changes in serum specific IgE against total L. perenne pollen extract (32). Thus, it cannot be excluded that reduced target-cell sensitivity to natural or experimental allergen exposure may be caused, at least in part, by a decrease in formation of specific IgE against a major allergen, which cannot be assessed by conventional RAST.…”

Allergen immunotherapy for allergic respiratory diseases

“…During immunotherapy for seasonal pollinosis there is an Initial increase of serum allergenspecific IgE without apparent ill effects, followed by blunting of seasonal increases in IgE. 23,95,96 Furthermore quantitative measurements of allergen-specific IgG subclasses in these patients showed an increase in allergen-specific IgG1 and IgG4 in the serum. However, there is a poor correlation between immunoreactive IgG and IgG4 levels as determined by ELISA and the clinical response to immunotherapy.…”

Grass Pollen Immunotherapy Induces an Allergen-Specific IgA2 Antibody Response Associated with Mucosal TGF-β Expression

“…Different immunological changes, putatively underlying clinical tolerance to further allergen exposure, have been observed following IT. These include increases in allergen-specific IgG, particularly of the IgG4 subclass (2), and modest reductions in specific IgE Abs (3). Effector inflammatory cells such as eosinophils, mast cells, and basophils are reduced in allergic mucosal tissues (4).…”