Allergen immunotherapy for allergic respiratory diseases

Cappella, Antonio; Durham, Stephen R.

doi:10.4161/hv.21629

Cited by 40 publications

(29 citation statements)

References 134 publications

(153 reference statements)

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“…A major problem of allergen-specific immunotherapy is the development of life-threating anaphylaxis during treatment (31,32). To circumvent this side effect, we postulated that encapsulation of the allergen (OVA) in a cationic liposome (DOTAP) would reduce the risks of anaphylaxis by preventing the allergen from the contact with allergen-specific IgE (33).…”

Section: Allergen (Ova) Encapsulation In a Cationic Liposome Attenuatmentioning

confidence: 99%

Allergen-Specific Immunotherapy With Liposome Containing CpG-ODN in Murine Model of Asthma Relies on MyD88 Signaling in Dendritic Cells

et al. 2020

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Changing the immune responses to allergens is the cornerstone of allergen immunotherapy. Allergen-specific immunotherapy that consists of repeated administration of increasing doses of allergen extract is potentially curative. The major inconveniences of allergen-specific immunotherapy include failure to modify immune responses, long-term treatment leading to non-compliance and the potential for developing life-threating anaphylaxis. Here we investigated the effect of a novel liposomal formulation carrying low dose of allergen combined with CpG-ODN, a synthetic TLR9 agonist, on established allergic lung inflammation. We found that challenge with allergen (OVA) encapsulated in cationic liposome induced significantly less severe cutaneous anaphylactic reaction. Notably, short-term treatment (three doses) with a liposomal formulation containing co-encapsulated allergen plus CpG-ODN, but not allergen or CpG-ODN alone, reversed an established allergic lung inflammation and provided long-term protection. This liposomal formulation was also effective against allergens derived from Blomia tropicalis mite extract. The attenuation of allergic inflammation was not associated with increased numbers of Foxp3-positive or IL-10-producing regulatory T cells or with increased levels of IFN-gamma in the lungs. Instead, the anti-allergic effect of the liposomal formulation was dependent of the innate immune signal transduction generated in CD11c-positive putative dendritic cells expressing MyD88 molecule. Therefore, we highlight the pivotal role of dendritic cells in mediating the attenuation of established allergic lung inflammation following immunotherapy with a liposomal formulation containing allergen plus CpG-ODN.

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Section: Allergen (Ova) Encapsulation In a Cationic Liposome Attenuatmentioning

confidence: 99%

Allergen-Specific Immunotherapy With Liposome Containing CpG-ODN in Murine Model of Asthma Relies on MyD88 Signaling in Dendritic Cells

et al. 2020

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“…Allergen-specific immunotherapy is the practice of administering gradually increasing quantities of allergen extracts to ameliorate the symptoms associated with the subsequent exposure to the causative allergen in order to induce a state of tolerance (Malling and Weeke, 1993;Bousquet et al, 1998;Cappella and Durham, 2012;Eifan et al, 2013;Canonica et al, 2014). It is considered to be a cure for allergic disease although symptomatic treatment such as antihistamines and nasal corticosteroids allows tentative relief from symptoms (Bousquet et al, 1998).…”

Section: Introductionmentioning

confidence: 97%

Safety evaluation of standardized allergen extract of Japanese cedar pollen for sublingual immunotherapy

Mitobe

Yokomoto

Ohashi-Doi³

2015

Regulatory Toxicology and Pharmacology

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“…The mechanisms by which clinical responses are achieved have been extensively described and include a decrease in basophils and mast cell activation within the affected tissues, induction of T cells anergy, generation of Th1 and Treg cells, decrease of allergen-specific IgE antibody levels, associated with the production of allergen-specific IgG 1 and IgG 4 antibodies (IgG “blocking activity”) and induction of the production of non-inflammatory IgA antibodies by B cells [20,22-24]. …”

Section: Introductionmentioning

confidence: 99%

Impact of allergic rhinitis and specific subcutaneous immunotherapy on peripheral blood basophils of patients sensitized to Dermatophagoides pteronyssinus

Lopes¹,

Azenha²,

Teodósio

et al. 2013

All Asth Clin Immun

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BackgroundBasophils are important effectors cells in allergic rhinitis (AR) since they are involved in immunoglobulin (Ig) E – mediated inflammation and in the release of pro-inflammatory mediators. Specific subcutaneous immunotherapy (SCIT) provides clear immunologic modulation in some immune cells, however its systemic effects on basophils are not well known.MethodsPeripheral blood (PB) samples from 43 patients with allergic rhinitis mono-sensitized to Dermatophagoides pteronyssinus (Dpt) [33 of them under SCIT with allergoid Dpt extract, in maintenance dose (SCIT), with evaluation just before SCIT injection (SCIT-T0) and 4 hours later (SCIT-T4) and the other 10 Dpt allergic patients never having, in the past, undergone specific immunotherapy treatment (NSIT)], and 15 healthy age- and gender-matched controls (HG), were analyzed. For each sample, the total (t-IgE) and specific IgE (s-IgE) was performed, as well as, the relative frequency and absolute number of PB basophils and receptor-bound IgE and IgG expression were evaluated by flow cytometry and the Histamine N-methyltransferase (HNMT) and tryptase α/β1 (TPSAB1) gene expression was assessed by real-time PCR.ResultsHigher levels of receptor-bound IgE were observed in SCIT patients, which are correlated with the levels of serum t-IgE and s-IgE, whereas no significant differences were observed for receptor-bound IgG. Regarding HNMT mRNA expression, significantly lower expression levels were detected in AR patients compared to HG, independently of type of therapy. Moreover a negative correlation was found between HNMT gene expression and time under SCIT. Conversely, tryptase gene expression was significantly up-regulated in NSIT when compared to HG; however in SCIT patients, tryptase gene expression was significantly decreased than in NSIT patients. No differences were found for any parameter between SCIT-T0 and SCIT-T4 with exception of a transient increased expression of tryptase in SCIT-T4.ConclusionPB basophils from patients with AR show altered functional features, which seems to be influenced by SCIT, suggesting that these cells could be useful to clarify the SCIT triggered mechanisms at a systemic level.

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Allergen immunotherapy for allergic respiratory diseases

Cited by 40 publications

References 134 publications

Allergen-Specific Immunotherapy With Liposome Containing CpG-ODN in Murine Model of Asthma Relies on MyD88 Signaling in Dendritic Cells

Allergen-Specific Immunotherapy With Liposome Containing CpG-ODN in Murine Model of Asthma Relies on MyD88 Signaling in Dendritic Cells

Safety evaluation of standardized allergen extract of Japanese cedar pollen for sublingual immunotherapy

Impact of allergic rhinitis and specific subcutaneous immunotherapy on peripheral blood basophils of patients sensitized to Dermatophagoides pteronyssinus

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