Measurement of endogenous uracil and dihydrouracil in plasma and urine of normal subjects by liquid chromatography–tandem mass spectrometry

Jiang, Hao; Jiang, Ji; Hu, Pei; Hu, Yufang

doi:10.1016/s1570-0232(02)00009-0

Cited by 41 publications

(44 citation statements)

References 14 publications

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“…In addition, the DHU/U ratio after continuous loading was 13.8 ± 6.0, while the ratios reported from France 21 and China 22 have been between 2 and 4, suggesting ethnic differences in uracil-metabolizing capacity and, consequently, 5-FU-metabolizing capacity. The results in group 2 indicate a high correlation between the DHU/U ratios before and after 5-FU administration.…”

Section: Measuring 5-fluorouracil Catabolic Potentialmentioning

confidence: 77%

Feasibility of Measuring 5-Fluorouracil Catabolic Potential by Oral Loading

et al. 2005

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The efficacy of 5-fluorouracil (5-FU) treatment and the incidence of adverse events differ among patients and depend to some extent on individual variations in drug catabolism. This feasibility study aimed to determine the optimum conditions for a 5-FU oral load test, which would allow the simple evaluation of individual differences in 5-FU catabolism. Patients with colon cancer were given oral 5-FU (200 mg/day) for 3 days (n = 36) or a single 100 mg dose (n = 14). Serum concentrations of uracil, dihydrouracil, 5-FU and 5-fluoro-5,6-dihydrouracil were measured before and after 5-FU administration. The results suggested that a decline in 5-FU metabolism was associated with continuous administration and increasing age. We conclude that a continuous load of 5-FU is necessary in order to predict the efficacy and side-effects of the drug. The 3-day regimen, with its ease of administration, merits further study to assess its possible clinical application.

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Section: Measuring 5-fluorouracil Catabolic Potentialmentioning

confidence: 77%

Feasibility of Measuring 5-Fluorouracil Catabolic Potential by Oral Loading

et al. 2005

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“…Separations of analyte from plasma were performed on a Discovery Amide C16 column and pretreatment of urine sample was done by centrifugation This method was helpful for the evaluation of DPD enzyme activity and for the adjustment of clinical dosages in pyrimidine chemotherapeutics with a limit of quantification (LOQ) of 0.5 ng mL -1 for Ura [12]. Ura and UH2 have been further quantified by using LC-UV and LC-MS methods of detections after SPE pre-treatment.…”

Section: Hybrid Separation Techniques Used For Sensitive Analysis Of mentioning

confidence: 99%

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

2015

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The most important objectives that are frequently found in bio-analytical chemistry involve applying tools to relevant medical/biological problems and refining these applications. Developing a reliable sample preparation step, for the medical and biological fields is another primary objective in analytical chemistry, in order to extract and isolate the analytes of interest from complex biological matrices. Since, main inborn errors of metabolism (IEM) diagnosable through uracil analysis and the therapeutic monitoring of toxic 5-fluoruracil (an important anticancerous drug) in dihydropyrimidine dehydrogenase deficient patients, require an ultra-sensitive, reproducible, selective, and accurate analytical techniques for their measurements. Therefore, keeping in view, the diagnostic value of uracil and 5-fluoruracil measurements, this article refines several analytical techniques involved in selective recognition and quantification of uracil and 5-fluoruracil from biological and pharmaceutical samples. The prospective study revealed that implementation of molecularly imprinted polymer as a solid-phase material for sample preparation and preconcentration of uracil and 5-fluoruracil had proven to be effective as it could obviates problems related to tedious separation techniques, owing to protein binding and drastic interferences, from the complex matrices in real samples such as blood plasma, serum samples.

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“…To date, U and/or UH 2 have been measured in biological matrices by gas chromatography-mass spectrometry (21 ) and by HPLC with UV detection (LC-UV) (26 -31 ) or mass spectrometry detection (LC-MS) (32)(33)(34). Only 4 of these methods include simultaneous measurement of U and UH 2 in plasma (26,27,30,34 ), but these methods have some limitations.…”

Section: -Fluorouracil (5-fu)mentioning

confidence: 99%

“…Only 4 of these methods include simultaneous measurement of U and UH 2 in plasma (26,27,30,34 ), but these methods have some limitations. Three of them have used substitution matrices for the validation: 80 g/L BSA (26 ), 30 g/L BSA (34 ), or water (27 ).…”

Section: -Fluorouracil (5-fu)mentioning

confidence: 99%

“…We collected blood into lithium heparin tubes at the same time of day (between 0900 and 1000) to minimize the influence of circadian variation in DPD activity (34 ) and immediately centrifuged the samples at 2000g and 4°C for 10 min to isolate plasma. Plasma samples were stored at Ϫ20°C until analysis (i.e., after 2-10 days of storage).…”

Section: Uh 2 /U Ratios In Cancer Patientsmentioning

confidence: 99%

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