Measurement of Concentration and Viability in Stallion Sperm

Love, Charles C.

doi:10.1016/j.jevs.2012.06.015

Cited by 10 publications

(9 citation statements)

References 20 publications

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“…). The term ‘live sperm’ indicated spermatozoa with intact membrane while ‘dead sperm' those with disrupted membrane as it is considered more appropriate to refer to the membrane intactness (Love ).…”

Section: Methodsmentioning

confidence: 99%

“…), among others. Eosin/nigrosin staining, the most commonly employed, has shown a good agreement with flow cytometry and automatic cell counter technique when used on semen with high levels of sperm membrane intactness (SMI), while for low levels of SMI, it tended to overestimate them (Love ). A simple dual staining procedure with Trypan blue/Giemsa has been used in different species with good results (Didion et al.…”

Section: Introductionmentioning

confidence: 99%

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Trypan Blue/Giemsa Staining to Assess Sperm Membrane Integrity in Salernitano Stallions and its Relationship to Pregnancy Rates

Serafini

Longobardi

Spadetta

et al. 2013

Reprod Domestic Animals

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Aim of this study was to test the reliability of Trypan blue/Giemsa staining to evaluate sperm membrane integrity, acrosomal intactness and morphology in stallion to verify whether it could be applied in vitro as useful tool for sperm fertilizing ability. Fertility data on inseminated mares were collected to evaluate the relationship of sperm quality to pregnancy rates. Forty-one ejaculates were collected from 3 stallions of Salernitano Horse Breed and evaluated for gross appearance, volume, visual motility and membrane integrity with Trypan blue/Giemsa staining and thirty-five mares were inseminated during the breeding season from April to July. Differences among stallions were found in volume, sperm concentration (p < 0.05) and visual motility (p < 0.01). A decrease in sperm motility, concentration (p < 0.05) and total sperm number was found in June-July (p < 0.01). Live sperm with intact acrosome (LSIA) and proximal droplets (PD) were lower (p < 0.01) in June-July, while acrosome reacted sperm (ARS) percentage increased (p < 0.05). No fertility differences were found among stallions with an average fertility per cycle of 44.6% and a pregnancy rate of 68.6%. Higher percentages of LSIA were found in the ejaculates used to inseminate mares that became pregnant vs those used in mares not pregnant (p < 0.05). The significance of LSIA as test variable to verify the reliability of Trypan blue/Giemsa staining was confirmed by Receiver operating characteristic ROC analysis and the sensitivity of the test was 85% at a cut-off value of 48% LSIA. Trypan blue-Giemsa showed to be an accurate method that can be applied on field to evaluate sperm membrane integrity and to identify poor-quality ejaculates.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trypan Blue/Giemsa Staining to Assess Sperm Membrane Integrity in Salernitano Stallions and its Relationship to Pregnancy Rates

Serafini

Longobardi

Spadetta

et al. 2013

Reprod Domestic Animals

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“…This approach makes it possible to use the NucleoCounter ® for measuring concentrations in undiluted raw semen and semen diluted in a wide variety of semen extenders including cooled/transported and frozen/thawed semen (Hansen et al, ; McKinnon et al, ). This method is currently considered as one of the most reliable systems for measuring concentration of stallion semen (Love, ). However, this instrumentation is expensive and not suitable for frequent transportation (McKinnon et al, ).…”

Section: Introductionmentioning

confidence: 99%

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration

Dini

Troch

Lemahieu

et al. 2019

Reprod Domestic Animals

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The objective of this study was to evaluate the accuracy of a novel, portable device (iSperm® Equine for assessing concentration and motility of stallion semen). In the first experiment, semen concentration was determined by the iSperm® Equine (Aidmics Biotechnology), Androvision® (Minitube) and NucleoCounter® SP‐100™ (ChemoMetec). The total motility and progressive motility were determined by the iSperm® Equine and the Androvision® using the manufacturer's guidelines. Frozen/thawed semen samples (n = 33) at various dilutions were analysed for concentration and motility with the above‐mentioned devices. There was a significant correlation between the concentrations measured with iSperm® and NucleoCounter® at all the measured dilutions. Moreover, <10% difference in concentrations was observed between the iSperm® and NucleoCounter® using the Bland–Altman test. There was also a significant correlation between iSperm® and Androvision® for total and progressive motility. In the second experiment, the parameters used in the Androvision® were modified to match those of the iSperm®. Total motility and progressive motility of frozen/thawed semen samples (n = 10) were determined, and the similarity between the Androvision® and iSperm® was confirmed by correlation studies and Bland–Altman test. The results of these experiments demonstrate that the iSperm® offers a reliable and practical alternative for the semi‐automated measurement of concentration and motility of stallion semen in the field. The iSperm® enables the practitioner to obtain objective and repeatable measurements on a variety of semen types (fresh, cooled and frozen) in the field at the time of insemination and thus acquire more insight into the quantity and quality of the provided insemination doses. This mare‐side diagnostic tool may help practitioners in identifying presumed subfertility problems more rapidly and act accordingly.

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“…However, even with the best cryopreservation protocols, fertility rates obtained using frozen semen from some stallions remain lower than those obtained with fresh and refrigerated semen, varying greatly between stallions (GOMES et al, 2009, LOVE, 2012) . An alternative to minimize sperm damage caused by freezing is the search for more effective cryoprotectants and additives, (HARTWIG et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Holt (2000) stated that there are differences in lipid composition of the sperm membrane between individuals of the same species, in the same and different races. In many species of mammals, more than 60 % of spermatic membrane fatty acids are long chain polyunsaturated fatty acids (PUFA) of the omega three (ω3) series (NISSEN & KREYSEL, 1983). This specific lipid composition confers greater fluidity to the plasma membrane due to the presence of some double bonds (OLIVEIRA et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The addition of the salmon oil in the freezing of equine semen

Agostinho

Andrade

Caiado

et al. 2017

Rev. bras. saúde prod. anim.

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SUMARY The aim of the present study was to verify the effect of salmon oil addition on cryopreservation of equine semen. The experiment consisted of two treatments. Treatment 1 (T1) (control diluent), BotuCrio® was used without addition of salmon oil and treatment 2 (T2) (experimental diluent) BotuCrio® plus (with) 2% salmon oil. Three ejaculates of four stallions were used, totalizing 12 collections (n=12). Overall motility and progressive motility were evaluated by the Hamilton Thorn Research (HTR) Ceros 10.8 program, as well as the plasma membrane functionality through the hyposmotic test. Both treatments did not present statistical differences in relation to motility (T1 25,2±1,7 a, T2 29,7±1,9 a) and progressive motility (T1 11,0±1,1 a, T2 14,1±1,3 a). With respect to the hyposmotic test, the treatment 2 plus 2% of Salmon oil, presented better protection of sperm membrane functionality in relation to the control treatment (T2 77,3±1,0 a, T1 68,0±1,0 b). It can be concluded that salmon oil, although not altering the total and progressive motility, confers a better efficiency of sperm membrane functionality after thawing in equine semen.

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Measurement of Concentration and Viability in Stallion Sperm

Cited by 10 publications

References 20 publications

Trypan Blue/Giemsa Staining to Assess Sperm Membrane Integrity in Salernitano Stallions and its Relationship to Pregnancy Rates

Trypan Blue/Giemsa Staining to Assess Sperm Membrane Integrity in Salernitano Stallions and its Relationship to Pregnancy Rates

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration

The addition of the salmon oil in the freezing of equine semen

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Measurement of Concentration and Viability in Stallion Sperm

Cited by 10 publications

References 20 publications

Trypan Blue/Giemsa Staining to Assess Sperm Membrane Integrity in Salernitano Stallions and its Relationship to Pregnancy Rates

Trypan Blue/Giemsa Staining to Assess Sperm Membrane Integrity in Salernitano Stallions and its Relationship to Pregnancy Rates

Validation of a portable device (iSperm®) for the assessment of stallion sperm motility and concentration

The addition of the salmon oil in the freezing of equine semen

Contact Info

Product

Resources

About

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration