Trypan Blue/Giemsa Staining to Assess Sperm Membrane Integrity in Salernitano Stallions and its Relationship to Pregnancy Rates

Serafini, R.; Longobardi, V.; Spadetta, M; Neri, Diana; Ariota, B.; Gasparrini, B.; Palo, R. Di

doi:10.1111/rda.12221

Cited by 28 publications

(18 citation statements)

References 41 publications

(47 reference statements)

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“…Sperm morphology was assessed in concert with trypan blue stain as described elsewhere. 54 Oil Red O (ORO) staining. The lipid droplets inside seminiferous tubules were visualized by ORO staining.…”

mentioning

confidence: 99%

Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells

Hou

et al. 2015

Cell Death Differ

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mentioning

confidence: 99%

Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells

Hou

et al. 2015

Cell Death Differ

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“…The sperms with no morphological alteration and inability to balance osmotic pressure indicated the damage of plasma membranes. The acrosome integrity of sperms was evaluated by Trypanblue Giemsa stain (Serafini et al 2013) of sperm morphology. After smearing and staining preparation, acrosome integrity was detected under the microscope using oil immersion.…”

Section: Semen Quality Analysismentioning

confidence: 99%

Effects of soy isoflavones on intake, body weight, sex hormones, antioxidant performance, and semen quality in Xinong Saanen goats

Xie

et al. 2021

Journal of Applied Animal Research

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Efficacy of soy isoflavones to promote semen quality by improving antioxidant parameters in Xinong Saanen goats was explored. Soy isoflavones (SI), regulator of steroid hormone synthesis and antioxidant in organism, was supplemented in a total mixed ration of male Xinong Saanen dairy goats to study their effects on intake, body weight, level of testosterone and oestradiol, antioxidant performance, and semen quality. Supplementation with SI increased (P < 0.05) dry matter intake expressed as kg/day or % of body weight, with body weight remaining unchanged. In animals receiving SI supplementation, we observed increases (P < 0.05) in serum superoxide dismutase and total antioxidant capacity and decreases in oestradiol and malondialdehyde concentrations in comparison to the Control, while testosterone and glutathione peroxidase remained unchanged. An increase (p < 0.05) in plasma membrane integrity of sperm were observed in bucks received SI supplementation with no other effects on semen quality such as seminal pH, ejaculate volume, viability, motility, and acrosome integrity. The results indicated that SI supplementation improved semen characteristics via enhancing antioxidant parameters. Highlights. Supplementation of soy isoflavones increased feed intake in Xinong Saanen bucks.. Soy isoflavones decreased serum oestradiol but not testosterone concentrations.. Soy isoflavones could be used as a safe, effective, and natural antioxidant in dairy goats. It alleviated oxidative stress, without negative influence on sperm quality of Xinong Saanen bucks.

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“…Existing methods for counting cardiomyocytes are mostly manual or semi-automated operations, such as the blood cell counting plate [25], the trypan blue staining count [26], and the visual field counting method [27]. Manual counting not only takes a long time but also incurs human errors.…”

Section: Related Workmentioning

confidence: 99%

Toward Automatic Cardiomyocyte Clustering and Counting through Hesitant Fuzzy Sets

et al. 2019

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The isolation and observation of cardiomyocytes serve as the fundamental approach to cardiovascular research. The state-of-the-practice for the isolation and observation relies on manual operation of the entire culture process. Such a manual approach not only incurs high human errors, but also takes a long period of time. This paper proposes a new computer-aided paradigm to automatically, accurately, and efficiently perform the clustering and counting of cardiomyocytes, one of the key procedures for evaluating the success rate of cardiomyocytes isolation and the quality of culture medium. The key challenge of the proposed method lies in the unique, rod-like shape of cardiomyocytes, which has been hardly addressed in literature. Our proposed method employs a novel algorithm inspired by hesitant fuzzy sets and integrates an efficient implementation into the whole process of analyzing cardiomyocytes. The system, along with the data extracted from adult rats’ cardiomyocytes, has been experimentally evaluated with Matlab, showing promising results. The false accept rate (FAR) and the false reject rate (FRR) are as low as 1.46% and 1.97%, respectively. The accuracy rate is up to 98.7%—20% higher than the manual approach—and the processing time is reduced from tens of seconds to 3–5 s—an order of magnitude performance improvement.

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Trypan Blue/Giemsa Staining to Assess Sperm Membrane Integrity in Salernitano Stallions and its Relationship to Pregnancy Rates

Cited by 28 publications

References 41 publications

Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells

Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells

Effects of soy isoflavones on intake, body weight, sex hormones, antioxidant performance, and semen quality in Xinong Saanen goats

Toward Automatic Cardiomyocyte Clustering and Counting through Hesitant Fuzzy Sets

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