Measurement of Carbohydrates in Soil Hydrolyzates With Anthrone

Brink, R.; Dubach, Paul; Lynch, D. L.

doi:10.1097/00010694-196003000-00006

Cited by 402 publications

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“…Soluble carbohydrate content was determined by reacting the nutrient solution with the anthrone reagent and analyzing for absorbance at 625 nm against glucose standards [22]. Total soluble carbohydrates were expressed as glucose equivalents per plant.…”

Section: Analyses Of Root Exudatesmentioning

confidence: 99%

Glyphosate affects soybean root exudation and rhizosphere micro-organisms

Kremer

Means

Kim

2005

International Journal of Environmental Analytical Chemistry

177

153

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Section: Analyses Of Root Exudatesmentioning

confidence: 99%

Glyphosate affects soybean root exudation and rhizosphere micro-organisms

Kremer

Means

Kim

2005

International Journal of Environmental Analytical Chemistry

177

153

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“…The soil total carbon and nitrogen contents were measured using an NC analyzer (NC-800, Sumitomo Chemical Co., Japan). The inorganic NH 4 and NO 3 in the extractant of 10 g fresh soil shaken with 100 mL 2M KCl for 1 h were determined using a flow-injection analyzer (Aquatec 5400, Tecator Co., Sweden). Soil water content was calculated by the weight difference before and after oven drying at 105 • C for 24 h. We determined the content of wax, cool-water-soluble polysaccharides, hot-watersoluble polysaccharides, hemicellulose, cellulose, and lignin in the soils of ten sites (HG3, AP, TZ, OD1, OD2, IB, ST, KH, HS and OK).…”

Section: Soil Analysismentioning

confidence: 99%

“…The contents in each fraction, except lignin, were expressed as the sum of pentose and hexose [20]. The pentose content was determined by orcinol method [19], and the hexose content was determined by anthrone method [4]. Once all the extraction were complete, carbon and nitrogen contents of the residue were measured using an NC analyzer and the lignin content was calculated using this equation: lignin content = carbon content × 1.724 -nitrogen content × 6.25.…”

Section: Soil Analysismentioning

confidence: 99%

Article

et al. 2006

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-The CO 2 fluxes from the forest floor were measured using a closed chamber method at 26 sites from 26 • N Lat. to 44 • N Lat. in Japan. Seasonal fluctuation in CO 2 flux was found to correlate exponentially with seasonal fluctuation in soil temperature at each site. Estimate of annual carbon emission from the forest floor ranged from 3.1 to 10.6 Mg C ha −1 . The emission rate of soil-organic-carbon-derived CO 2 , obtained by incubation of intact soil samples, correlated closely with the carboxymethylcellulase (CMCase) activity in the soil. The sum of cool-water soluble polysaccharides, hot-water soluble polysaccharides, hemicellulose, and cellulose content in the soil was greater at the sites with low CMCase activity than that at the sites with high CMCase activity. Because the sites in cooler-climate sites had a high content of easily decomposable soil organic carbon and organic litter, the potential increase in CO 2 efflux from forest floor with increasing soil temperature would be greater in cooler-climate sites.cellulose / Japanese forest / soil organic carbon / soil respiration Résumé -Fort potentiel d'accroissement de flux de CO 2 issu de la surface du sol forestier en relation avec le réchauffement global dans les régions fraîches du Japon. Le flux de CO 2 issu du sol forestier a été mesuré dans 26 sites, allant du 26 • au 44 • de latitude Nord dans l'archipel japonais, en utilisant la méthode des chambres fermées. Il a pu être mis en évidence que la fluctuation saisonnière du flux de CO 2 était corrélée de façon exponentielle avec celle de la température du sol de chacun des sites étudiés. L'estimation annelle de l'émission de carbone venant du sol variait de 3,1 à 10,6 Mg C ha −1 . Le taux d'émission de CO 2 obtenu par incubation d'échantillons intacts de sol est corrélé positivement avec l'activité de la carboxyméthacellulase (CMCase), dans le sol. La somme totale des polysaccharides solubles dans l'eau froide, des polysaccharides solubles dans l'eau chaude, des hémicelluloses et de la cellulose contenus dans le sol était plus grande dans les sites caractérisés par une faible activité CMCase que dans les sites avec une forte activité CMCase. Du fait que les sites en climat frais ont un contenu élevé en carbone organique du sol facilement décomposable et une litière organique, le potentiel d'accroissement du flux de CO 2 avec l'accroissement de la température du sol devrait être plus grand dans les sites à climat frais.cellulose / forêt japonaise / carbone organique du sol / respiration du sol

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“…The solution was cooled to room temperature, and then adjusted to pH 7.0 with 6.0 mol L -1 NaOH. The extract was centrifuged for 10 min at 24,0009g to collect the supernatant [46], and then the total carbohydrate content was determined using the enthrone sulfuric acid spectrophotometric method described by Brink et al [8].…”

Section: Soluble Protein and Total Carbohydratesmentioning

confidence: 99%

Enhanced Resistance to UV-B Radiation in Anabaena sp. PCC 7120 (Cyanophyceae) by Repeated Exposure

Qin

2014

Curr Microbiol

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In natural habitats, organisms especially phytoplankton are not always continuously subjected to ultraviolet-B radiation (UVBR). By simulation of the natural situation, the N 2 -fixing cyanobacterium Anabaena sp. PCC 7120 was subjected to UV-B exposure and recovery cycles. A series of morphological and physiological changes were observed in Anabaena sp. PCC 7120 under repeated UVBR when compared with controls. Such as the breakage of filaments, intervals between heterocysts, heterocyst frequency, total carbohydrate, and carotenoids were increased, while the nitrogenase activity and photosynthetic activity were inhibited by repeated UVBR; however, these activities could recover when UV-B stress was removed. Unexpectedly, the over-compensatory growth was observed at the end of the second round of exposure and recovery cycle. Our results showed that discontinuous UVBR could increase the growth rate and the tolerance as well as repair capacity of Anabaena sp. PCC 7120. These results indicate that moderate UVBR may increase the growth of cyanobacteria in natural habitats.

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Measurement of Carbohydrates in Soil Hydrolyzates With Anthrone

Cited by 402 publications

References 0 publications

Glyphosate affects soybean root exudation and rhizosphere micro-organisms

Glyphosate affects soybean root exudation and rhizosphere micro-organisms

Article

Enhanced Resistance to UV-B Radiation in Anabaena sp. PCC 7120 (Cyanophyceae) by Repeated Exposure

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