Mean Centering of Ratio Spectra Method for the Analysis of Theophylline and Ephedrine HCL Mixture in Tablet

Muchlisyam, Muchlisyam; Sudarmi, Sudarmi; Caroline, Cindy

doi:10.22159/ajpcr.2018.v11i5.24365

Cited by 3 publications

(1 citation statement)

References 4 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various studies on the development of Spectrophotometry methods have been published as examples: UV spectrophotometry methods for simultaneous estimation, Double Divisor Spectra derivative spectrophotometry ratio, and MCR method [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Assays of Metformin HCL and Glibenclamide Mixture Using Two Analytical Methods of Spectrophotometry

Bachri

2019

Int J App Pharm

View full text Add to dashboard Cite

Objective: Area Under Curve method (AUC) and the Multiple Wavelength Spectrophotometric (MWS) method are practice and simple methods for simultaneous assays of Metformin HCl and Glibenclamide on the tablet dosage form. Methods: The AUC method is measured for the absorption spectrum with a concentration 4 mg/l Metformin HCl by calculating the area spectrum at wavelength 230-240 nm and the absorption spectrum with a concentration 8.7 mg/l Glibenclamide by calculating the area at wavelength 225-235 nm. The MWS by determining the absorption spectrum and the five wavelength points for the absorption value at 225 nm, 229.4 nm, 236.6 nm, 233 nm, and 243 nm and calculated using matrix operations. Results: The validation test of the AUC method for Metformin HCl obtained accuracy = 99.35%, linearity = 0.9881, precision = 0.39%, LOD = 0.4459 mg/l. LOQ = 1.4864 mg/l and for Glibenclamide obtained accuracy = 100.79%, linearity = 0.9993, precision = 0.65%, LOD=0.4372 mg/l, LOQ = 1.4072 mg/l and the MWS method for Metformin HCl obtained accuracy = 100.76%, linearity 0.9949, precision = 0.65%, LOD = 0.9103 mg/l, LOQ = 3.0431 mg/l, and for Glibenclamide with accuracy = 100.07%, linearity = 0.9993. precision = 0.36%, LOD = 0.9205 mg/l. LOQ = 3.0431 mg/l , and appropriate the requirements of ICH guidelines. Conclusion: These Methods successively applied to determine of Metformin HCl and Glibenclamide mixture in tablet dosage form and fulfill the validation requirements.

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous Assays of Metformin HCL and Glibenclamide Mixture Using Two Analytical Methods of Spectrophotometry

Bachri

2019

Int J App Pharm

View full text Add to dashboard Cite

show abstract

Development and validation of mean centering ratio spectra spectrophotometry method for binary mixture of glimepiride and metformin hydrochloride in tablet disage form

Tarigan¹,

Silitonga²,

Sari³

et al. 2023

AIP Conference Proceedings

View full text Add to dashboard Cite

APPLICATION MEAN CENTERING RATIO SPECTRA METHOD FOR TERNARY MIXTURE OF DEXTROMETHORPHAN HBr, DOXYLAMINE SUCCINATE, AND PSEUDOEPHEDRINE HCl IN TABLET PREPARATIONS

TARIGAN,

FAISAL,

LASE

et al. 2024

Int J App Pharm

View full text Add to dashboard Cite

Objective: The aim of this research is to apply the mean Centering Ratio Spectra (MCRS) method for a ternary mixture of Dextromethorphan Hydrobromide (DE), Doxylamine Succinate (DO), and Pseudoephedrine Hydrochloride (PS) in tablet preparations. Methods: The absorption spectra of DE (40-430 μg/ml), DO (20-430 μg/ml), and PS (230-630 μg/ml) were scanned in the 200-400 nm range. DE determination involved dividing DE spectra by PS (430 μg/ml) and mean-centered the ratio spectra. DO spectra were divided by DE (40 μg/ml) to obtain mean-centered ratio spectra. The mean-centered ratio spectra for PS were obtained by dividing the scanned spectra of its prepared solutions by the standard spectrum of DE, which had a concentration of 70 μg/ml. Results: Method validation parameters, including linearity, accuracy, precision, limit of Detection (LOD), and limit of Quantification (LOQ), were assessed. The results indicated linearity values of 0.9988, 0.9992, and 0.9998, respectively, for DE, DO, and PS. Accuracy: 99.84%, 100.15%, and 100.33%, respectively, for DE, DO, and PS. Precision: 0.53%, 0.54%, and 0.74%, respectively, for DE, DO, and PS. lOD: 1.85 μg/ml, 2.09 μg/ml, and 1.56 μg/ml, respectively, for DE, DO, and PS. lOQ: 6.19 μg/ml, 6.97 μg/ml, and 5.22 μg/ml, respectively, for DE, DO, and PS. All validation method parameters meet International Conference of Harmonization (ICH) validation requirements. Conclusion: The MCRS method can be applied for the simultaneous ternary mixtures of DE, DO, and PS in tablet preparations. Its successful application, combined with rigorous validation results, suggests its potential for adoption in routine pharmaceutical analysis, contributing to the advancement of quality control practices in the industry.

show abstract

Mean Centering of Ratio Spectra Method for the Analysis of Theophylline and Ephedrine HCL Mixture in Tablet

Cited by 3 publications

References 4 publications

Simultaneous Assays of Metformin HCL and Glibenclamide Mixture Using Two Analytical Methods of Spectrophotometry

Simultaneous Assays of Metformin HCL and Glibenclamide Mixture Using Two Analytical Methods of Spectrophotometry

Development and validation of mean centering ratio spectra spectrophotometry method for binary mixture of glimepiride and metformin hydrochloride in tablet disage form

APPLICATION MEAN CENTERING RATIO SPECTRA METHOD FOR TERNARY MIXTURE OF DEXTROMETHORPHAN HBr, DOXYLAMINE SUCCINATE, AND PSEUDOEPHEDRINE HCl IN TABLET PREPARATIONS

Contact Info

Product

Resources

About