Mean Centering Method for determination of Empagliflozin and Metformin

Ayoub, Bassam M.; Emam, Rola M; Youssef, Mahmoud; El-Kattan, Muhammad N; Sayed, Marwa A; Kowider, Ahmed M; Seha, Adly H; Rabea, Engy A; Yakout, Rana M; Faried, Rolly H

doi:10.12991/marupj.323590

Cited by 15 publications

(5 citation statements)

References 5 publications

(10 reference statements)

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“…Few other analytical methods were described for the simultaneous determination of LIG in combination with other antidiabetic agents using HPTLC (El-Kimary et al, 2016;Patel et al, 2020), UPLC/ UV (Ayoub, 2015;Vankalapati et al, 2021), and LC-MS/MS (Al Bratty et al, 2017;Rathod et al, 2020) methods, either in their pharmaceutical dosage form or in human plasma. In addition, other methods were reported for the determination of EMG either alone (Abdel-Ghany et al, 2018;Ayoub, Emam, et al, 2017;Manoel et al, 2021) or in combination with MEF Munde et al, 2020;Riaz et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Application of experimental design in HPLC method optimization and robustness for the simultaneous determination of canagliflozin, empagliflozin, linagliptin, and metformin in tablet

Moussa

Mahrouse

Fawzy

2021

Biomedical Chromatography

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Gliflozins and gliptins represent two different pharmacological drug classes that exert different and potentially complementary glucose-lowering effect in patients with type II diabetes mellitus. A novel, selective, and sensitive HPLC method was developed for the determination of canagliflozin, empaglifozin, linagliptin, and metformin in pure form, in laboratory prepared mixtures, and in pharmaceutical dosage form. Experimental design optimization was applied by using Plackett-Burman and face-centered composite designs to achieve the best resolution with minimum experimental trials. Three significant variables affecting optimization, namely buffer pH, percentage of methanol, and percentage of acetonitrile, were studied. Chromatographic separation was achieved using an Agilent Eclipse C 8 column, and column temperature was kept at 45 C. The mobile phase was composed of dipotassium hydrogen phosphate buffer (0.05 M, adjusted to pH 6 using o-phosphoric acid):acetonitrile:methanol (50:25:25, v/v/v) at a flow rate of 1.5 mL/min. Sharp and wellresolved peaks of the cited drugs were obtained. The method was fully validated in terms of linearity, accuracy, precision, selectivity and robustness in agreement with the International Council of Harmonization (ICH) guidelines Q2 (R1). Satisfactory results were obtained by the analysis of tablets through applying the developed method. Therefore, it could be performed for the analysis of the cited drugs in quality control laboratories.

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Section: Introductionmentioning

confidence: 99%

Application of experimental design in HPLC method optimization and robustness for the simultaneous determination of canagliflozin, empagliflozin, linagliptin, and metformin in tablet

Moussa

Mahrouse

Fawzy

2021

Biomedical Chromatography

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“…A review of the literature shows that there are several methods for the separate determination of metformin HCl and empagliflozin in tablet dosage form by UV spectrophotometer [5][6][7], RP-HPLC [8][9][10][11][12][13][14][15], UPLC [16,17], and some analytical instruments. LC-MS/MS [18].…”

Section: Figure 2 Chemical Structure Of Empagliflozinmentioning

confidence: 99%

A review on method development and validation of stability indicating RP HPLC Method for metformin and Empagliflozin

Vinayak A. katekar,

Prafful P. Kothari,

Swapnil S. Kawarkhe

et al. 2023

GSC Biol. Pharm. Sci.

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Metformin HCl and empagliflozin are oral antidiabetic medications that help control blood sugar levels. A simple, precise, rapid, accurate, sensitive, specific, and stable RP-HPLC method was developed and validated for the simultaneous determination of Metformin and Empagliflozin in drug bulk form and drug dosage. This new RP-HPLC method is superior to formal reversed-phase HPLC with reduced solvent usage, retention time, resolution, and cost. Upper region separation was performed on a Waters HPLC system equipped with a PDA detector and autosampler. Both Metformin and Empagliflozin undergo stress conditions including acid, alkali, oxidative, thermal, and photodegradation, but significant degradation was observed in acid studies. The newly developed RP HPLC chromatographic method was validated for system compatibility, linearity, robustness, accuracy, and precision.

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“…In UV spectrophotometry, the excitation of electrons present in the sample molecules occurs easily by absorption of high energy of light in the range of 200-800 nm. The molecules with conjugated pielectrons are able to absorb the radiation in this region [11].In this type of spectroscopy reflection or transmission properties of molecules are used for the quantitative analysis as a function of wavelength, for example, glibenclamide [46,47], metformin [8,48], acarbose [49], miglitol [49,50], alogliptin [50], linagliptin [51], saxagliptin [52], Sitagliptin [53], nateglinide [54], repaglinide [55], dapagliflozin, [56], canagliflozin [57], empagliflozin [58], glimepiride [59], glipizide [60], and chlorpropamide [61].…”

Section: Ultraviolet-visible Spectroscopymentioning

confidence: 99%

Pharmaceutical Analysis and Diabetes Mellitus - A Review

Venkataraman

Manasa

2018

Asian J Pharm Clin Res

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Context: Current review focuses on the importance of pharmaceutical analytical techniques in the diagnosis of diabetes and the study of antidiabetic drugs. Objective: The main objective of the review is to compile the different analytical tools such as UV-spectroscopy, Fourier-transform infrared (FT-IR), nuclear magnetic resonance (NMR), liquid chromatography-mass spectrometry (LC-MS), gas chromatography (GC)-MS, and electrophoresis that are used to diagnose diabetes using different analytical methods. It also attempts to provide information on the assay of antidiabetic drugs by various analytical techniques and thus helps in the development of novel methods for the study of newer drugs molecules having antidiabetic activity. Methods: The blood sample is subjected to centrifugation to collect the serum before any analytical estimation of antidiabetic drugs. The different types of diabetes were diagnosed using various analytical techniques such as UV-spectroscopy, FT-IR, NMR, LC-MS, GC-MS, and electrophoresis. Results: Different analytical methods that were reported in the literature, were succinctly discussed about the quantification of various antidiabetic drugs. These methods were also employed for the determination of antidiabetic drugs in the research field for the study of actives pharmaceutical ingredients, marketing dosage forms, and post-marketed dosage forms. Conclusion: This review is compiled in such a manner such that it helps the analysts to diagnose diabetes using different analytical techniques and also to study various antidiabetic drugs in the pharmaceutical research field. It also attempts to provide information about different active pharmaceutical ingredients, marketing dosage forms, and post-marketed dosage forms.

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Mean Centering Method for determination of Empagliflozin and Metformin

Cited by 15 publications

References 5 publications

Application of experimental design in HPLC method optimization and robustness for the simultaneous determination of canagliflozin, empagliflozin, linagliptin, and metformin in tablet

Application of experimental design in HPLC method optimization and robustness for the simultaneous determination of canagliflozin, empagliflozin, linagliptin, and metformin in tablet

A review on method development and validation of stability indicating RP HPLC Method for metformin and Empagliflozin

Pharmaceutical Analysis and Diabetes Mellitus - A Review

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