Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological Samples

Abstract

In the present study, a rapid, practical, selective and sensitive method was proposed for simultaneous determination of dopamine (DA) and uric acid (UA). Kinetic profiles of analytes were recorded and transformed by different mother wavelets. The contribution of ascorbic acid was neglected using mean centering of data before transformation. The concentrations range were 1.0–60.0 μmol L−1 and 7–40.0 μmol L−1, with detection limit of 0.06 and 0.30 μmol L−1 for uric acid and dopamine, respectively. The results of sym8 and db4 mother wavelets showed that proposed method creates a most selective and sensitive determination without using initial separation steps. The obtained results by CWT-sym8 and CWT-db4 were compared with partial least squares (PLS) results. The simultaneous quantitation of DA and UA with the proposed method was successfully applied in different urine and serum samples.

Green and efficient magnetic micro-solid phase extraction utilizing tea waste impregnated with magnetic nanoparticles for the analysis of ibuprofen in water samples by using UV-vis spectrophotometry

“…However, due to the low sensitivity factor of the UV-Vis spectrophotometer, a preconcentration-separation technique is required for precise and accurate measurements. [9][10][11] Therefore, the enrichment and purication of IBP have been achieved using a variety of sample preparation techniques. Drugs and their metabolites are typically determined using the liquid-liquid extraction (LLE), solid phase extraction (SPE), stir rod sorptive extraction (SRSE), dispersive liquid-liquid microextraction (DLLME), and solid phase micro-extraction (SPME) procedures.…”

“…Iron oxides such as magnetite (Fe 3 O 4 ) were employed as magnetic nanoparticles (MNPs) due to their great characteristics, including simple preparation methods and effectiveness in separating target analytes from samples, which are frequently used as sorbents. 11,15 Even with the large volume of samples, the use of MNPs greatly reduces extraction time and mass sorbent consumption. 17,18 They guarantee high extraction capacity, dynamic extraction, and extraction efficiency.…”

“…17,18 They guarantee high extraction capacity, dynamic extraction, and extraction efficiency. 11 Even though MNPs have great characteristics, unmodied MNPs are unstable in an acidic medium; thus, different strategies can be used to change the surface of MNPs through modications to overcome these restrictions. 19,20 Many waste products from industrial and agricultural processes can be used again to create more value for more affordable and efficient adsorbents.…”

“…However, due to the low sensitivity factor of the UV-Vis spectrophotometer, a pre-concentration-separation technique is required for precise and accurate measurements. 9–11…”