mcr-1 plasmid-mediated colistin resistance gene detection in an Enterobacter cloacae clinical isolate in France

Baron, Sophie Alexandra; Bardet, Lucie; Dubourg, Grégory; Fichaux, Marie; Rolain, Jean‐Marc

doi:10.1016/j.jgar.2017.05.004

Cited by 19 publications

(13 citation statements)

References 6 publications

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“…cloacae were only reported from Asia [ 38 , 39 ]; however, the geographic range was expanded with the discovery of a clinical E . cloacae isolate with mcr -1 in France [ 40 ]. The mcr -3 harboring E .…”

Section: Resultsmentioning

confidence: 99%

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

Wise¹,

Estabrook²,

Sahm³

et al. 2018

PLoS ONE

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A set of 908 clinically derived colistin-resistant Enterobacteriaeae isolates collected worldwide in 2014–2016 were screened for the presence of the plasmid-borne mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 genes. In total 3.2% (29/908) of the collection were positive for mcr, including 27 Escherichia coli, 1 Klebsiella pneumoniae and 1 Enterobacter cloacae. Twenty-four isolates possessed genes from the mcr-1 family, including the original mcr-1 (n = 22), as well as mcr-1.2 (n = 1) and mcr-1.5 (n = 1), which each differ from mcr-1 by encoding single amino acid variations. Genes from the mcr-3 family were found in isolates from Thailand, including mcr-3.1 (n = 3) and mcr-3.2 (n = 1). An E. coli isolated from a patient with a urinary tract infection in Colombia contained the recently discovered mcr-5. The full colistin-resistant collection was tested against a panel of antimicrobial agents with ceftazidime-avibactam and tigecycline exhibiting the highest activity.

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Section: Resultsmentioning

confidence: 99%

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

Wise¹,

Estabrook²,

Sahm³

et al. 2018

PLoS ONE

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“…However, this isolate did not harbor mcr - 1 or mcr - 2 genes. Similarly, colistin-resistant E. cloacae isolates with or without the mcr - 1 gene have been found in healthy people or primary care patients [ 18 – 20 ]. Currently, the mechanism of colistin resistance in E. cloacae strains in the absence of plasmid-borne colistin resistance genes ( mcr - 1 to - 5 ) remains poorly understood.…”

Section: Resultsmentioning

confidence: 99%

Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia

et al. 2018

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Camels (Camelus dromedarius) are known to harbor multidrug resistant Gram-negative bacteria and to be involved in the transmission of various microorganisms to humans. Data on the occurrence of colistin resistant Escherichia coli as well as mobilized colistin resistance (mcr) genes in camels are lacking. We investigated the presence of colistin resistance and mcr (1–2) genes in E. coli from the feces of camels in Tunisia. Presumptive E. coli isolates from camel-calves in southern Tunisia were qualitatively screened for growth on Mueller–Hinton agar supplemented with 2 mg/L of colistin. The minimal inhibitory concentration of colistin was determined for isolates growing on this medium. All isolates were screened for the presence of the mcr-1 and mcr-2 genes by polymerase chain reaction without detecting any of these genes. However, one isolate was confirmed resistant to colistin and further testing of this isolate revealed it to be Enterobacter cloacae. Our study demonstrated absence of colistin resistance and of the mcr-1 and mcr-2 genes in E. coli isolated from camel feces in southern Tunisia. Thus, there is no evidence that camels represent a major source of mcr genes contamination for the local population or for tourists visiting southern Tunisia.

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“…As reported by Mirelis, the total resistance rate of Enterobacteriaceae to colistin was 0.67% but the resistance rate of Enterobacter cloacae 4.2% was much higher than that of Escherichia coli and Klebsiella pneumoniae (23). The resistance mechanism of ECC strains to colistin can be mediated by the mcr-1 gene carried by the plasmid (15,24,25), and may also be related to the mutations of two-component system encoded by pmrAB and phoPQ (13). Recently, Zong et al found that a novel small transmembrane protein Ecr may mediate the heterogeneous resistance of ECC to colistin through the PhoPQ two-component system (16), but the distribution of ecr gene in ECC strains and its correlation with colistin resistance still need further study.…”

Section: Discussionmentioning

confidence: 66%

“…Because of the good activity against Gram-negative bacteria, it is considered to be one of the last-line antimicrobials for treatment of MDR Gram-negative bacteria (14). According to previous reports, ECC may acquire colistin resistance by plasmid-mediated gene mcr, small protein gene ecr or two-component systems phoPQ, pmrAB (13,15,16). The emergence of carbapenem-resistant and colistin-resistant ECC will undoubtedly pose a huge challenge to infection control.…”

Section: Introductionmentioning

confidence: 99%

Characterization of resistance mechanisms of Enterobacter cloacae Complex Co-resistant to Carbapenem and Colistin

Zhou

Liu

Fang

et al. 2020

Preprint

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Background: The emergence of carbapenem-resistant and colistin-resistant ECC pose a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in Enterobacter cloacae Complex (ECC) strains.Methods: Nineteen ECC isolates co-resistant to carbapenems and colistin were collected from a regional medical center in China. Carbapenemase gene, extended-spectrum β-lactamase gene, AmpC cephalosporinase gene ampC, mcr series genes, and ecr gene were detected by PCR. Expression levels of outer membrane protein OmpC/OmpF and efflux pump protein AcrA/AcrB were investigated. And the structural modification of lipid A of 19 ECC strains was analyzed.Results: This study showed that the mechanisms of carbapenem resistance in this study are: 1. Generating carbapenemase (7 of 19); 2. The production of AmpC or ESBLs combined with decreased expression of out membrane protein (12 of 19). And the mechanism of colistin resistance is increaseing expression of acrA in the efflux pump AcrAB-TolC alone (5 of 19) or accompanied by a decrease of affinity between colistin and outer membrane caused by the modification of lipid A (14 of 19). Moreover, an ECC strain co-harboring plasmid-mediated mcr-4.3 and blaNDM-1 has been found.Conclusions: This study suggested that there is no overlap between the resistance mechanism of co-resistant ECC strains to carbapenem and colistin. However, the emergence of strain co-harboring plasmid-mediated resistance genes indicated that ECC is a potential carrier for the horizontal spread of carbapenems and colistin resistance.

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mcr-1 plasmid-mediated colistin resistance gene detection in an Enterobacter cloacae clinical isolate in France

Cited by 19 publications

References 6 publications

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia

Characterization of resistance mechanisms of Enterobacter cloacae Complex Co-resistant to Carbapenem and Colistin

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