Maxillary Bone Regeneration Based on Nanoreservoirs Functionalizedε-Polycaprolactone Biomembranes in a Mouse Model of Jaw Bone Lesion

Abstract

Current approaches of regenerative therapies constitute strategies for bone tissue reparation and engineering, especially in the context of genetical diseases with skeletal defects. Bone regeneration using electrospun nanofibers' implant has the following objectives: bone neoformation induction with rapid healing, reduced postoperative complications, and improvement of bone tissue quality. In vivo implantation of polycaprolactone (PCL) biomembrane functionalized with BMP-2/Ibuprofen in mouse maxillary defects was followed by bone neoformation kinetics evaluation using microcomputed tomography. Wild-Type (WT) and Tabby (Ta) mice were used to compare effects on a normal phenotype and on a mutant model of ectodermal dysplasia (ED). After 21 days, no effect on bone neoformation was observed in Ta treated lesion (4% neoformation compared to 13% in the control lesion). Between the 21st and the 30th days, the use of biomembrane functionalized with BMP-2/Ibuprofen in maxillary bone lesions allowed a significant increase in bone neoformation peaks (resp., +8% in mutant Ta and +13% in WT). Histological analyses revealed a neoformed bone with regular trabecular structure, areas of mineralized bone inside the membrane, and an improved neovascularization in the treated lesion with bifunctionalized membrane. In conclusion, PCL functionalized biomembrane promoted bone neoformation, this effect being modulated by the Ta bone phenotype responsible for an alteration of bone response.

Tissue engineering applications in otolaryngology—The state of translation

“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate craniofacial tissue engineering 49‐72,74‐77,79,81‐84,145‐197 …”

Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications

“…Angiogenesis-related factors, such as vascular endothelial growth factor (VEGF) and hypoxia inducible factor (HIF) 1α, can significantly promote osteoblast differentiation and osteogenesis. Thus, effective vascularization is essential for promoting bone defect repair and functional restoration [16][17][18].…”

“…Bone engineering Nano-HA (1) In vitro study (2) In vivo glucocorticoid-induced bone defect model [28] In vitro study [29,33,154,155] In vivo ectopic osteogenesis study [18] In vivo calvarial defect model [20,99] Micro/Nano-structured surfaces of Cu x -HA (1) In vitro study (2) In vivo subcutaneously implant study [34] TCP nanolayers In vitro study [21] Nanofibrin In vitro study [24] GO In vivo calvarial defect study [22,26] In vivo ectopic osteogenesis study [27] PCL nanofibrous biomembranes In vivo maxillary bone lesion model [16] Mesoporous bioactive glass nanoparticles (1) In vitro study (2) In vivo ectopic osteogenesis study [30] Copper doped in electrospun bioactive glass nanofibers…”

Temporomandibular Joint Regenerative Medicine

“…This strategy also allows the differentiation of MSCs, and accelerates the tissue regeneration in vivo [ 147 , 148 , 149 ]. Besides, co-functionalization allowed by nanoreservoirs on nanofibers can promote regeneration but also normalization of inflammation at the implantation site [ 150 ] ( Figure 5 B).…”