Mature-embryo culture in the cryopreservation of passion fruit (Passiflora edulis Sims) seeds

Generoso, Andressa Leal; Carvalho, Virgínia Silva; Walter, Rafael; Campbell, Glaziele; Araújo, Letícia da Silva; Santana, Josefa Grasiela Silva; Cunha, Maura Da

doi:10.1016/j.scienta.2019.108638

Cited by 11 publications

(7 citation statements)

References 21 publications

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“…In liquid nitrogen storage (-196 °C), all metabolic activity and cell divisions are interrupted, preventing the degradation of the stored material and contamination (Cruz -Cruz et al 2013). Direct immersion in liquid nitrogen has the advantage of not requiring the use of cryoprotectants in materials with tolerance to cooling and low moisture content, as seen in the present study, and with several examples, such as the one seen for Dyckia brevifolia Beker and Dyckia delicata Larocca & Sobral seeds (De Paula et al 2020), mature seeds of Cattleya guttata Lindl (Vettorazzi et al 2019) and for Passiflora edulis Sims seeds (Generoso et al 2019). Although the present study hasn't observed a difference between the storage treatments, it is assumed that cryopreservation has more long-term advantages when compared to storage in a refrigerator, in which there may be degradation and contamination of the material in the medium term.…”

Section: Discussionsupporting

confidence: 50%

Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

Montoya-Serrano

Vesco

Pescador

2022

Hoehnea

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Vriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate the cryopreservation using the method of direct immersion of its seeds, collected from capsules at 120, 135, and 150 days after anthesis (DAA). The water content was determined before cryopreservation, while the germination percentage, germination speed index (GSI), and total soluble carbohydrates were quantified after cryopreservation. The highest percentage of moisture (17.6%) was observed in 120 DAA, while the highest percentage of germination (89.6%) and GSI (17.0) were observed in 150 DAA. Optical and transmission electron microscopy analyses were performed, and no cell damage or changes at the morpho-histological and ultrastructural levels were observed after the cryopreservation process. From these results, V. reitzii seeds can be classified as orthodox seeds and the cryopreservation (+LN) is an efficient tool for an ex situ conservation of this species.

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Section: Discussionsupporting

confidence: 50%

Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

Montoya-Serrano

Vesco

Pescador

2022

Hoehnea

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“…Previous studies developed by our group indicate the possibility of the cryopreservation of P. edulis seeds efficiently by reducing the water content to values close to 10% (Generoso et al, 2019).…”

Section: Introductionmentioning

confidence: 87%

New evidence for in vitro conservation of nodal segments of the passion fruit ‘UENF Rio Dourado’ (Passiflora edulis Sims)

et al. 2023

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The germplasm of Passiflora L. is conserved through seed banks and field collections. Dormant seeds, seeds with low viability, and high-cost field collections make it difficult to maintain germplasm banks, and in vitro conservation can be a complementary alternative. The aim of this study was to investigate the survival of nodal segments of Passiflora edulis Sims ‘UENF Rio Dourado’ over 180 days of slow growth in vitro by reducing the mineral salt and sucrose concentrations and changing the incubation conditions (temperature and light intensity). The experiment was conducted in a completely randomized design with a 2 × 3 × 3 factorial arrangement consisting of two temperatures (20 ± 2°C and 27 ± 2°C), three concentrations of MSM mineral salts (100% MSM, 50% MSM, and 25% MSM), and three sucrose concentrations (10, 20, and 30 g L-1). Evaluations were performed at 60, 90, 120, 150, and 180 days for survival, number of leaves, and plant color (using a color scale where 1 = dark green, 2 = light green, and 3 = yellow). After 180 days of culture, mineral salt, sucrose concentration, and incubation temperature affected plant survival, regeneration, and acclimatization. During the 180 days, it was possible to slow the growth of nodal segments of passion fruit ‘UENF Rio Dourado’ in culture medium with 25% MSM mineral salts plus 10 g L-1 sucrose, at an average temperature of 20°C without compromising plant survival, regeneration, and acclimatization.

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“…In Passiflora edulis, the seeds with initial moisture content of 25 per cent were desiccated to various moisture levels (15, 10 and 5 %) by thin layer drying at 40 ℃ for different durations (42, 70 and 100 minutes respectively and exposed to liquid nitrogen treatment (Generoso et al, 2019). The cryopreserved seeds with water content of 10% produced all normal seedlings under in vitro while non-desiccated seeds did not produce normal seedlings.…”

Section: Dehydration Freezingmentioning

confidence: 99%

Cryopreservation of Fruit Genetic Resources-A Review

Anushma¹,

Dhanyasree²,

Rafeekher³

2020

Int.J.Curr.Microbiol.App.Sci

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Conserving the plant genetic resources through any means is of utmost importance to prevent the loss of plant biodiversity. Long-term storage using liquid nitrogen (LN) is usually adopted for developing long-term base collections of crops for use in the distant future. The fruit crops are commonly propagated through vegetative means and are majorly conserved as ex situ field gene banks, which require large areas and demand intensive field management. The need for repeated reculturing and chances of contamination during subcultures in in vitro banking necessitate a long-term contamination free conservation through cryobanking. Improved cryopreservation techniques will provide added security in the preservation of important woody plant germplasm formerly preserved only as field collections.

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Mature-embryo culture in the cryopreservation of passion fruit (Passiflora edulis Sims) seeds

Cited by 11 publications

References 21 publications

Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

New evidence for in vitro conservation of nodal segments of the passion fruit ‘UENF Rio Dourado’ (Passiflora edulis Sims)

Cryopreservation of Fruit Genetic Resources-A Review

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