Maturation of dendritic cells by bacterial immunomodulators

Dendritic cell activation and maturation induced by mucosal fluid from women with bacterial vaginosis

“…The level of CD83 is correlated with maturation of MDDCs [23,25] while an increase in CD40 or HLA-DR indicate activation of MDDC [25,26]. The mean fluorescence intensity (MFI) of all surface markers (CD83, CD40 and HLA-DR) was significantly increased due to BV CVL (57%, 62% and 52% of LPS respectively) when compared to normal CVL (29%, 22% and 9% of LPS) (Fig.…”

“…Endocytosis was measured by uptake of FITC-dextran with molecular weight of 40,000 (Sigma-Aldrich, St.Louis, MO) and was similar to the methods used by Spisek et al [23]. MDDC (200,000 cells/ml) were incubated in medium containing 0.5mg/ml FITC-dextran for 120min at 37°C or at 4°C (control).…”

Transcriptome analyses extend understanding of Streptococcus pyogenes regulatory mechanisms and behavior toward immunomodulatory substances

“…Its clinical efficacy in the respiratory tract has been demonstrated in several randomized, prospective studies (Jareoncharsri et al, 2003;Ruah et al, 2001;Steurer-Stey et al, 2004). Besides exerting a general not well-understood effect on macrophage and T-helper cell activity and increasing g-interferon and secretory IgA production (Ruedl et al, 1994;Spisek et al, 2004), the complex substance could include compounds with growth-inhibitory and/or gene expression-modulating effects for S. pyogenes isolates.…”

Polybacterial immunomodulator Respivax restores the inductive function of innate immunity in patients with recurrent respiratory infections

“…Further on, the immunomodulatory Streptococcal preparation OK432 induced phenotypic and functional maturation of human monocyte-derived DCs [50]. In still another in vitro study, Luivac, Biostim and Ribomunyl-treated human DCs displayed activated phenotype, reduced phagocytic activity and increased potential for stimulating the proliferation of allogeneic Tlymphocytes [14]. We studied for the first time the effects of the polybacterial immunomodulator Respivax on the maturation/activation state of circulating APC ex-vivo.…”

“…In 2004, similar polybacterial preparations, Luivac, Biostim and Ribomunil were shown to have a strong stimulating in vitro effect on dendritic cells [14]. Up to now there is no data available about the direct ex vivo influence of polybacterial immunomodulators on innate immunity mechanisms.…”